Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test

The purpose of this study was to analyze the in vitro antimicrobial activity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%). Bacterial samples (ATCC) of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis and Fusobacterium nucleatum wer...

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Main Authors: Sassone Luciana Moura, Fidel Rivail Antonio Sergio, Fidel Sandra Rivera, Dias Marina, Hirata Junior Raphael
Format: Article
Language:English
Published: Fundação Odontológica de Ribeirão Preto 2003-01-01
Series:Brazilian Dental Journal
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402003000200005
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author Sassone Luciana Moura
Fidel Rivail Antonio Sergio
Fidel Sandra Rivera
Dias Marina
Hirata Junior Raphael
author_facet Sassone Luciana Moura
Fidel Rivail Antonio Sergio
Fidel Sandra Rivera
Dias Marina
Hirata Junior Raphael
author_sort Sassone Luciana Moura
collection DOAJ
description The purpose of this study was to analyze the in vitro antimicrobial activity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%). Bacterial samples (ATCC) of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis and Fusobacterium nucleatum were submitted to a contact test. Solutions were evaluated at different time intervals: immediately, 5 min, 15 min, and 30 min after contact and repeated 10 times. The results of the contact test showed that 0.12% chlorhexidine did not eliminate E. faecalis at any time interval, while 0.5% and 1% chlorhexidine and 1% and 5% sodium hypochlorite did. These results permit us to conclude that to obtain better antimicrobial activity, chlorhexidine in a concentration greater than 0.12% should be used.
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spelling doaj.art-76dd9a7317714c23b51fe299f76f7be42022-12-21T19:13:01ZengFundação Odontológica de Ribeirão PretoBrazilian Dental Journal0103-64402003-01-0114299102Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact testSassone Luciana MouraFidel Rivail Antonio SergioFidel Sandra RiveraDias MarinaHirata Junior RaphaelThe purpose of this study was to analyze the in vitro antimicrobial activity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%). Bacterial samples (ATCC) of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis and Fusobacterium nucleatum were submitted to a contact test. Solutions were evaluated at different time intervals: immediately, 5 min, 15 min, and 30 min after contact and repeated 10 times. The results of the contact test showed that 0.12% chlorhexidine did not eliminate E. faecalis at any time interval, while 0.5% and 1% chlorhexidine and 1% and 5% sodium hypochlorite did. These results permit us to conclude that to obtain better antimicrobial activity, chlorhexidine in a concentration greater than 0.12% should be used.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402003000200005antimicrobial activitysodium hypochloritechlorhexidineendodontics
spellingShingle Sassone Luciana Moura
Fidel Rivail Antonio Sergio
Fidel Sandra Rivera
Dias Marina
Hirata Junior Raphael
Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test
Brazilian Dental Journal
antimicrobial activity
sodium hypochlorite
chlorhexidine
endodontics
title Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test
title_full Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test
title_fullStr Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test
title_full_unstemmed Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test
title_short Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test
title_sort antimicrobial activity of different concentrations of naocl and chlorhexidine using a contact test
topic antimicrobial activity
sodium hypochlorite
chlorhexidine
endodontics
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402003000200005
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