Microtubules provide directional information for core PCP function
Planar cell polarity (PCP) signaling controls the polarization of cells within the plane of an epithelium. Two molecular modules composed of Fat(Ft)/Dachsous(Ds)/Four-jointed(Fj) and a ‘PCP-core’ including Frizzled(Fz) and Dishevelled(Dsh) contribute to polarization of individual cells. How polarity...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2014-08-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/02893 |
| _version_ | 1811253314511175680 |
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| author | Maja Matis David A Russler-Germain Qie Hu Claire J Tomlin Jeffrey D Axelrod |
| author_facet | Maja Matis David A Russler-Germain Qie Hu Claire J Tomlin Jeffrey D Axelrod |
| author_sort | Maja Matis |
| collection | DOAJ |
| description | Planar cell polarity (PCP) signaling controls the polarization of cells within the plane of an epithelium. Two molecular modules composed of Fat(Ft)/Dachsous(Ds)/Four-jointed(Fj) and a ‘PCP-core’ including Frizzled(Fz) and Dishevelled(Dsh) contribute to polarization of individual cells. How polarity is globally coordinated with tissue axes is unresolved. Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton. Here, we provide evidence for the model that the Ft/Ds/Fj-module provides directional information to the core-module through this MT organizing function. We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation. Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization. |
| first_indexed | 2024-04-12T16:48:58Z |
| format | Article |
| id | doaj.art-76dde61be8d54d37bb4e5722d9daea27 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:48:58Z |
| publishDate | 2014-08-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-76dde61be8d54d37bb4e5722d9daea272022-12-22T03:24:28ZengeLife Sciences Publications LtdeLife2050-084X2014-08-01310.7554/eLife.02893Microtubules provide directional information for core PCP functionMaja Matis0David A Russler-Germain1Qie Hu2Claire J Tomlin3Jeffrey D Axelrod4Department of Pathology, Stanford University School of Medicine, Stanford, United StatesDepartment of Pathology, Stanford University School of Medicine, Stanford, United StatesDepartment of Electrical Engineering and Computer Sciences, University of California, Berkeley, Berkeley, United StatesDepartment of Electrical Engineering and Computer Sciences, University of California, Berkeley, Berkeley, United StatesDepartment of Pathology, Stanford University School of Medicine, Stanford, United StatesPlanar cell polarity (PCP) signaling controls the polarization of cells within the plane of an epithelium. Two molecular modules composed of Fat(Ft)/Dachsous(Ds)/Four-jointed(Fj) and a ‘PCP-core’ including Frizzled(Fz) and Dishevelled(Dsh) contribute to polarization of individual cells. How polarity is globally coordinated with tissue axes is unresolved. Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton. Here, we provide evidence for the model that the Ft/Ds/Fj-module provides directional information to the core-module through this MT organizing function. We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation. Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.https://elifesciences.org/articles/02893planar cell polarityfat cadherinmicrotubules |
| spellingShingle | Maja Matis David A Russler-Germain Qie Hu Claire J Tomlin Jeffrey D Axelrod Microtubules provide directional information for core PCP function eLife planar cell polarity fat cadherin microtubules |
| title | Microtubules provide directional information for core PCP function |
| title_full | Microtubules provide directional information for core PCP function |
| title_fullStr | Microtubules provide directional information for core PCP function |
| title_full_unstemmed | Microtubules provide directional information for core PCP function |
| title_short | Microtubules provide directional information for core PCP function |
| title_sort | microtubules provide directional information for core pcp function |
| topic | planar cell polarity fat cadherin microtubules |
| url | https://elifesciences.org/articles/02893 |
| work_keys_str_mv | AT majamatis microtubulesprovidedirectionalinformationforcorepcpfunction AT davidarusslergermain microtubulesprovidedirectionalinformationforcorepcpfunction AT qiehu microtubulesprovidedirectionalinformationforcorepcpfunction AT clairejtomlin microtubulesprovidedirectionalinformationforcorepcpfunction AT jeffreydaxelrod microtubulesprovidedirectionalinformationforcorepcpfunction |