Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods
The aim of this study was to identify the etiological agents in patients with suspected onychomycosis, and to carry out comparative testing of individual or combinations of tests: direct microscopy with KOH and Blankophor (BP), culturing on Sabouraud’s dextrose agar (SDA), diluted Sabouraud...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2014-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641402587D.pdf |
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| author | Dubljanin Eleonora Džamić A.M. Mitrović Sanja Arsić-Arsenijević Valentina Čolović-Čalovski Ivana |
| author_facet | Dubljanin Eleonora Džamić A.M. Mitrović Sanja Arsić-Arsenijević Valentina Čolović-Čalovski Ivana |
| author_sort | Dubljanin Eleonora |
| collection | DOAJ |
| description | The aim of this study was to identify the etiological agents in patients with
suspected onychomycosis, and to carry out comparative testing of individual
or combinations of tests: direct microscopy with KOH and Blankophor (BP),
culturing on Sabouraud’s dextrose agar (SDA), diluted Sabouraud’s dextrose
agar (D-SDA) and dermatophyte test medium (DTM). From 70 nail samples (65
toenails, 5 fingernails), 46 (60.5%) had at least one of five positive tests.
Isolation was possible in 41, while in 5 samples the presence of fungi was
observed by KOH and/or BP. Dermatophytes were most frequently isolated
(80.5%) where Trichophyton rubrum was predominant. Candida spp. was isolated
in 9.8%, Aspergillus spp. 4.9%, Alternaria spp. 2.4% and Fusarium spp. 2.4%.
Application of BP as an individual test was the most sensitive method. The
combination of BP with DTM or D-SDA provides the best sensitivity and allows
the identification of fungi to the species/genus level. |
| first_indexed | 2024-12-12T03:31:21Z |
| format | Article |
| id | doaj.art-76e6bec3845d44f88928b836f0bc8625 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-12T03:31:21Z |
| publishDate | 2014-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-76e6bec3845d44f88928b836f0bc86252022-12-22T00:39:55ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392014-01-0166258759410.2298/ABS1402587D0354-46641402587DOnychomycosis: Clinical findings, etiological agents and evaluation of laboratory methodsDubljanin Eleonora0Džamić A.M.1Mitrović Sanja2Arsić-Arsenijević Valentina3Čolović-Čalovski Ivana4Faculty of Medicine, Institute of Microbiology and Immunology, BelgradeFaculty of Medicine, Institute of Microbiology and Immunology, BelgradeFaculty of Medicine, Institute of Microbiology and Immunology, BelgradeFaculty of Medicine, Institute of Microbiology and Immunology, BelgradeFaculty of Medicine, Institute of Microbiology and Immunology, BelgradeThe aim of this study was to identify the etiological agents in patients with suspected onychomycosis, and to carry out comparative testing of individual or combinations of tests: direct microscopy with KOH and Blankophor (BP), culturing on Sabouraud’s dextrose agar (SDA), diluted Sabouraud’s dextrose agar (D-SDA) and dermatophyte test medium (DTM). From 70 nail samples (65 toenails, 5 fingernails), 46 (60.5%) had at least one of five positive tests. Isolation was possible in 41, while in 5 samples the presence of fungi was observed by KOH and/or BP. Dermatophytes were most frequently isolated (80.5%) where Trichophyton rubrum was predominant. Candida spp. was isolated in 9.8%, Aspergillus spp. 4.9%, Alternaria spp. 2.4% and Fusarium spp. 2.4%. Application of BP as an individual test was the most sensitive method. The combination of BP with DTM or D-SDA provides the best sensitivity and allows the identification of fungi to the species/genus level.http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641402587D.pdfOnychomycosisBlankophorfungal culturedermatophytesnondermatophyte moldsSerbia |
| spellingShingle | Dubljanin Eleonora Džamić A.M. Mitrović Sanja Arsić-Arsenijević Valentina Čolović-Čalovski Ivana Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods Archives of Biological Sciences Onychomycosis Blankophor fungal culture dermatophytes nondermatophyte molds Serbia |
| title | Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods |
| title_full | Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods |
| title_fullStr | Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods |
| title_full_unstemmed | Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods |
| title_short | Onychomycosis: Clinical findings, etiological agents and evaluation of laboratory methods |
| title_sort | onychomycosis clinical findings etiological agents and evaluation of laboratory methods |
| topic | Onychomycosis Blankophor fungal culture dermatophytes nondermatophyte molds Serbia |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641402587D.pdf |
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