The genetic variability of grapevine Pinot gris virus (GPGV) in Australia
Abstract Grapevine Pinot gris virus (GPGV; genus Trichovirus in the family Betaflexiviridae) was detected in Australia in 2016, but its impact on the production of nursery material and fruit in Australia is still currently unknown. This study investigated the prevalence and genetic diversity of GPGV...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-023-02171-3 |
| _version_ | 1797355989021753344 |
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| author | Kamalpreet Kaur Amy Rinaldo David Lovelock Brendan Rodoni Fiona Constable |
| author_facet | Kamalpreet Kaur Amy Rinaldo David Lovelock Brendan Rodoni Fiona Constable |
| author_sort | Kamalpreet Kaur |
| collection | DOAJ |
| description | Abstract Grapevine Pinot gris virus (GPGV; genus Trichovirus in the family Betaflexiviridae) was detected in Australia in 2016, but its impact on the production of nursery material and fruit in Australia is still currently unknown. This study investigated the prevalence and genetic diversity of GPGV in Australia. GPGV was detected by reverse transcription-polymerase chain reaction (RT-PCR) in a range of rootstock, table and wine grape varieties from New South Wales, South Australia, and Victoria, with 473/2171 (21.8%) samples found to be infected. Genomes of 32 Australian GPGV isolates were sequenced and many of the isolates shared high nucleotide homology. Phylogenetic and haplotype analyses demonstrated that there were four distinct clades amongst the 32 Australian GPGV isolates and that there were likely to have been at least five separate introductions of the virus into Australia. Recombination and haplotype analysis indicate the emergence of new GPGV strains after introduction into Australia. When compared with 168 overseas GPGV isolates, the analyses suggest that the most likely origin of Australian GPGV isolates is from Europe. There was no correlation between specific GPGV genotypes and symptoms such as leaf mottling, leaf deformation, and shoot stunting, which were observed in some vineyards, and the virus was frequently found in symptomless grapevines. |
| first_indexed | 2024-03-08T14:19:12Z |
| format | Article |
| id | doaj.art-76eb519b4f244ff892e1e68313c61c07 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-03-08T14:19:12Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-76eb519b4f244ff892e1e68313c61c072024-01-14T12:12:01ZengBMCVirology Journal1743-422X2023-09-0120111710.1186/s12985-023-02171-3The genetic variability of grapevine Pinot gris virus (GPGV) in AustraliaKamalpreet Kaur0Amy Rinaldo1David Lovelock2Brendan Rodoni3Fiona Constable4School of Applied Systems Biology, La Trobe UniversityThe Australian Wine Research InstituteAgriculture Victoria Research, Department of Energy, Environment and Climate ActionSchool of Applied Systems Biology, La Trobe UniversitySchool of Applied Systems Biology, La Trobe UniversityAbstract Grapevine Pinot gris virus (GPGV; genus Trichovirus in the family Betaflexiviridae) was detected in Australia in 2016, but its impact on the production of nursery material and fruit in Australia is still currently unknown. This study investigated the prevalence and genetic diversity of GPGV in Australia. GPGV was detected by reverse transcription-polymerase chain reaction (RT-PCR) in a range of rootstock, table and wine grape varieties from New South Wales, South Australia, and Victoria, with 473/2171 (21.8%) samples found to be infected. Genomes of 32 Australian GPGV isolates were sequenced and many of the isolates shared high nucleotide homology. Phylogenetic and haplotype analyses demonstrated that there were four distinct clades amongst the 32 Australian GPGV isolates and that there were likely to have been at least five separate introductions of the virus into Australia. Recombination and haplotype analysis indicate the emergence of new GPGV strains after introduction into Australia. When compared with 168 overseas GPGV isolates, the analyses suggest that the most likely origin of Australian GPGV isolates is from Europe. There was no correlation between specific GPGV genotypes and symptoms such as leaf mottling, leaf deformation, and shoot stunting, which were observed in some vineyards, and the virus was frequently found in symptomless grapevines.https://doi.org/10.1186/s12985-023-02171-3GPGVMetagenomic HTSPhylogenetic analysisRecombinationMedian joining networkPopulation genetics |
| spellingShingle | Kamalpreet Kaur Amy Rinaldo David Lovelock Brendan Rodoni Fiona Constable The genetic variability of grapevine Pinot gris virus (GPGV) in Australia Virology Journal GPGV Metagenomic HTS Phylogenetic analysis Recombination Median joining network Population genetics |
| title | The genetic variability of grapevine Pinot gris virus (GPGV) in Australia |
| title_full | The genetic variability of grapevine Pinot gris virus (GPGV) in Australia |
| title_fullStr | The genetic variability of grapevine Pinot gris virus (GPGV) in Australia |
| title_full_unstemmed | The genetic variability of grapevine Pinot gris virus (GPGV) in Australia |
| title_short | The genetic variability of grapevine Pinot gris virus (GPGV) in Australia |
| title_sort | genetic variability of grapevine pinot gris virus gpgv in australia |
| topic | GPGV Metagenomic HTS Phylogenetic analysis Recombination Median joining network Population genetics |
| url | https://doi.org/10.1186/s12985-023-02171-3 |
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