Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO<sub>2</sub> Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform
This study proposes synthesis and evaluation of gelatin-/alginate-based hydrogel scaffolds reinforced with titanium dioxide (TiO<sub>2</sub>) nanoparticles which, through their combination with allantoin, quercetin, and caffeic acid, provide multi-target therapy directed on all phases of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/16/3/372 |
| _version_ | 1797239663793012736 |
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| author | Marija M. Babić Radić Marija Vukomanović Jasmina Nikodinović-Runić Simonida Tomić |
| author_facet | Marija M. Babić Radić Marija Vukomanović Jasmina Nikodinović-Runić Simonida Tomić |
| author_sort | Marija M. Babić Radić |
| collection | DOAJ |
| description | This study proposes synthesis and evaluation of gelatin-/alginate-based hydrogel scaffolds reinforced with titanium dioxide (TiO<sub>2</sub>) nanoparticles which, through their combination with allantoin, quercetin, and caffeic acid, provide multi-target therapy directed on all phases of the wound healing process. These scaffolds provide the simultaneous release of bioactive agents and concurrently support cell/tissue repair through the replicated structure of a native extracellular matrix. The hydrogel scaffolds were synthesized via a crosslinking reaction using EDC as a crosslinker for gelatin. Synthesized hydrogel scaffolds and the effect of TiO<sub>2</sub> on their properties were characterized by structural, mechanical, morphological, and swelling properties, and the porosity, wettability, adhesion to skin tissue, and simultaneous release features. The biocompatibility of the scaffolds was tested in vitro on fibroblasts (MRC5 cells) and in vivo (<i>Caenorhabditis elegans</i>) in a survival probe. The scaffolds revealed porous interconnected morphology, porosity of 88.33 to 96.76%, elastic modulus of 1.53 to 4.29 MPa, full hydrophilicity, favorable skin adhesivity, and biocompatibility. The simultaneous release was investigated in vitro indicating dependence on the scaffold’s composition and type of bioactive agents. The novel scaffolds designed as multi-target therapy have significant promise for improved wound healing in a beneficial and non-invasive manner. |
| first_indexed | 2024-04-24T17:55:07Z |
| format | Article |
| id | doaj.art-76eb5c3f673f4f109b40a18f571144e8 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-24T17:55:07Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-76eb5c3f673f4f109b40a18f571144e82024-03-27T13:59:44ZengMDPI AGPharmaceutics1999-49232024-03-0116337210.3390/pharmaceutics16030372Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO<sub>2</sub> Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy PlatformMarija M. Babić Radić0Marija Vukomanović1Jasmina Nikodinović-Runić2Simonida Tomić3University of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, SerbiaAdvanced Materials Department, Jožef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, SloveniaUniversity of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, 11000 Belgrade, SerbiaUniversity of Belgrade, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000 Belgrade, SerbiaThis study proposes synthesis and evaluation of gelatin-/alginate-based hydrogel scaffolds reinforced with titanium dioxide (TiO<sub>2</sub>) nanoparticles which, through their combination with allantoin, quercetin, and caffeic acid, provide multi-target therapy directed on all phases of the wound healing process. These scaffolds provide the simultaneous release of bioactive agents and concurrently support cell/tissue repair through the replicated structure of a native extracellular matrix. The hydrogel scaffolds were synthesized via a crosslinking reaction using EDC as a crosslinker for gelatin. Synthesized hydrogel scaffolds and the effect of TiO<sub>2</sub> on their properties were characterized by structural, mechanical, morphological, and swelling properties, and the porosity, wettability, adhesion to skin tissue, and simultaneous release features. The biocompatibility of the scaffolds was tested in vitro on fibroblasts (MRC5 cells) and in vivo (<i>Caenorhabditis elegans</i>) in a survival probe. The scaffolds revealed porous interconnected morphology, porosity of 88.33 to 96.76%, elastic modulus of 1.53 to 4.29 MPa, full hydrophilicity, favorable skin adhesivity, and biocompatibility. The simultaneous release was investigated in vitro indicating dependence on the scaffold’s composition and type of bioactive agents. The novel scaffolds designed as multi-target therapy have significant promise for improved wound healing in a beneficial and non-invasive manner.https://www.mdpi.com/1999-4923/16/3/372hydrogel scaffoldsgelatin-/alginate-based scaffoldssustained releasewound healingallantoincaffeic acid |
| spellingShingle | Marija M. Babić Radić Marija Vukomanović Jasmina Nikodinović-Runić Simonida Tomić Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO<sub>2</sub> Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform Pharmaceutics hydrogel scaffolds gelatin-/alginate-based scaffolds sustained release wound healing allantoin caffeic acid |
| title | Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO<sub>2</sub> Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform |
| title_full | Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO<sub>2</sub> Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform |
| title_fullStr | Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO<sub>2</sub> Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform |
| title_full_unstemmed | Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO<sub>2</sub> Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform |
| title_short | Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO<sub>2</sub> Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform |
| title_sort | gelatin alginate based hydrogel scaffolds reinforced with tio sub 2 sub nanoparticles for simultaneous release of allantoin caffeic acid and quercetin as multi target wound therapy platform |
| topic | hydrogel scaffolds gelatin-/alginate-based scaffolds sustained release wound healing allantoin caffeic acid |
| url | https://www.mdpi.com/1999-4923/16/3/372 |
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