Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell Lines
Three steroids, ergosterol (1), ergosterol peroxide (2) and stigmasterol (3) have been isolated from endophytic fungus, Lasiodiplodia theobromae derived from the root of Aglaia argentea Blume. The steroids were isolated by vacuum chromatography and column chromatography, the chemical structure was e...
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| Format: | Article |
| Language: | English |
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Jenderal Soedirman University
2023-11-01
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| Series: | Molekul |
| Online Access: | http://jos.unsoed.ac.id/index.php/jm/article/view/7824 |
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| author | Unang Supratman Sari Purbaya Desi Harneti Asri Peni Wulandari Yeni Mulyani Azmi Azhari Aprilia Permata Sari |
| author_facet | Unang Supratman Sari Purbaya Desi Harneti Asri Peni Wulandari Yeni Mulyani Azmi Azhari Aprilia Permata Sari |
| author_sort | Unang Supratman |
| collection | DOAJ |
| description | Three steroids, ergosterol (1), ergosterol peroxide (2) and stigmasterol (3) have been isolated from endophytic fungus, Lasiodiplodia theobromae derived from the root of Aglaia argentea Blume. The steroids were isolated by vacuum chromatography and column chromatography, the chemical structure was established following the analysis of 1D-NMR, 2D-NMR, IR, MS and by comparison with previously reported spectra data. Ergosterol peroxide (2) and stigmasterol (3) were reported for the first time isolated from Lasiodiplodia theobromae endophytic fungus. Cytotoxic activities of the compounds were tested with resazurin assay against HeLa cervical cancer cells, compound 2 displayed strongest cytotoxic activities against HeLa cervical cancer cells with IC50 values of 0.28 µM, while compounds 1 and 3 showed IC50 values of 0.34 µM and 27.32 µM, respectively |
| first_indexed | 2024-04-24T13:07:22Z |
| format | Article |
| id | doaj.art-76eba30a26a247f9ba3659d9bf6a4f0d |
| institution | Directory Open Access Journal |
| issn | 1907-9761 2503-0310 |
| language | English |
| last_indexed | 2024-04-24T13:07:22Z |
| publishDate | 2023-11-01 |
| publisher | Jenderal Soedirman University |
| record_format | Article |
| series | Molekul |
| spelling | doaj.art-76eba30a26a247f9ba3659d9bf6a4f0d2024-04-05T07:00:24ZengJenderal Soedirman UniversityMolekul1907-97612503-03102023-11-0118339640310.20884/1.jm.2023.18.3.78247824Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell LinesUnang Supratman0Sari Purbaya1Desi Harneti2Asri Peni Wulandari3Yeni MulyaniAzmi Azhari4Aprilia Permata Sari5Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaDepartment of Biology, Universitas PadjadjaranDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Sumedang, IndonesiaThree steroids, ergosterol (1), ergosterol peroxide (2) and stigmasterol (3) have been isolated from endophytic fungus, Lasiodiplodia theobromae derived from the root of Aglaia argentea Blume. The steroids were isolated by vacuum chromatography and column chromatography, the chemical structure was established following the analysis of 1D-NMR, 2D-NMR, IR, MS and by comparison with previously reported spectra data. Ergosterol peroxide (2) and stigmasterol (3) were reported for the first time isolated from Lasiodiplodia theobromae endophytic fungus. Cytotoxic activities of the compounds were tested with resazurin assay against HeLa cervical cancer cells, compound 2 displayed strongest cytotoxic activities against HeLa cervical cancer cells with IC50 values of 0.28 µM, while compounds 1 and 3 showed IC50 values of 0.34 µM and 27.32 µM, respectivelyhttp://jos.unsoed.ac.id/index.php/jm/article/view/7824 |
| spellingShingle | Unang Supratman Sari Purbaya Desi Harneti Asri Peni Wulandari Yeni Mulyani Azmi Azhari Aprilia Permata Sari Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell Lines Molekul |
| title | Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell Lines |
| title_full | Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell Lines |
| title_fullStr | Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell Lines |
| title_full_unstemmed | Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell Lines |
| title_short | Steroids Produced by Endophytic Fungus Lasiodiplodia Theobromae From Aglaia Argentea Blume and Their Cytotoxic Activity Against Hela Cervical Cancer Cell Lines |
| title_sort | steroids produced by endophytic fungus lasiodiplodia theobromae from aglaia argentea blume and their cytotoxic activity against hela cervical cancer cell lines |
| url | http://jos.unsoed.ac.id/index.php/jm/article/view/7824 |
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