Cancer cell invasion alters the protein profile of extracellular vesicles
Abstract Extracellular vesicles (EVs) are important mediators of intercellular communication involved in local and long‐range signalling of cancer metastasis. The onset of invasion is the key step of the metastatic cascade, but the secretion of EVs has remained unexplored at that stage due to techni...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-12-01
|
| Series: | Journal of Extracellular Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jex2.124 |
| _version_ | 1827577746998427648 |
|---|---|
| author | Jens C. Luoto Leila S. Coelho‐Rato Cecilia Jungarå Sara H. Bengs Jannica Roininen John E. Eriksson Lea Sistonen Eva Henriksson |
| author_facet | Jens C. Luoto Leila S. Coelho‐Rato Cecilia Jungarå Sara H. Bengs Jannica Roininen John E. Eriksson Lea Sistonen Eva Henriksson |
| author_sort | Jens C. Luoto |
| collection | DOAJ |
| description | Abstract Extracellular vesicles (EVs) are important mediators of intercellular communication involved in local and long‐range signalling of cancer metastasis. The onset of invasion is the key step of the metastatic cascade, but the secretion of EVs has remained unexplored at that stage due to technical challenges. In this study, we present a platform to track EVs over the course of invasive development of human prostate cancer cell (PC3) tumoroids utilizing in vivo‐mimicking extracellular matrix‐based 3D cultures. Using this EV production method, combined with proteomic profiling, we show that PC3 tumoroids secrete EVs with previously undefined protein cargo. Intriguingly, an increase in EV amounts and extensive changes in the EV protein composition were detected upon invasive transition of the tumoroids. The changes in EV protein cargo were counteracted by chemical inhibition of invasion. These results reveal the impact of the tumoroids’ invasive status on EV secretion and cargo, and highlight the necessity of in vivo‐mimicking conditions for uncovering novel cancer‐derived EV components. |
| first_indexed | 2024-03-08T21:32:15Z |
| format | Article |
| id | doaj.art-76eca7fb0c87468bb4c873159fc6b382 |
| institution | Directory Open Access Journal |
| issn | 2768-2811 |
| language | English |
| last_indexed | 2024-03-08T21:32:15Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Biology |
| spelling | doaj.art-76eca7fb0c87468bb4c873159fc6b3822023-12-21T07:04:28ZengWileyJournal of Extracellular Biology2768-28112023-12-01212n/an/a10.1002/jex2.124Cancer cell invasion alters the protein profile of extracellular vesiclesJens C. Luoto0Leila S. Coelho‐Rato1Cecilia Jungarå2Sara H. Bengs3Jannica Roininen4John E. Eriksson5Lea Sistonen6Eva Henriksson7Faculty of Science and Engineering, Cell Biology Åbo Akademi University Turku FinlandFaculty of Science and Engineering, Cell Biology Åbo Akademi University Turku FinlandFaculty of Science and Engineering, Cell Biology Åbo Akademi University Turku FinlandFaculty of Science and Engineering, Cell Biology Åbo Akademi University Turku FinlandFaculty of Science and Engineering, Cell Biology Åbo Akademi University Turku FinlandFaculty of Science and Engineering, Cell Biology Åbo Akademi University Turku FinlandFaculty of Science and Engineering, Cell Biology Åbo Akademi University Turku FinlandFaculty of Science and Engineering, Cell Biology Åbo Akademi University Turku FinlandAbstract Extracellular vesicles (EVs) are important mediators of intercellular communication involved in local and long‐range signalling of cancer metastasis. The onset of invasion is the key step of the metastatic cascade, but the secretion of EVs has remained unexplored at that stage due to technical challenges. In this study, we present a platform to track EVs over the course of invasive development of human prostate cancer cell (PC3) tumoroids utilizing in vivo‐mimicking extracellular matrix‐based 3D cultures. Using this EV production method, combined with proteomic profiling, we show that PC3 tumoroids secrete EVs with previously undefined protein cargo. Intriguingly, an increase in EV amounts and extensive changes in the EV protein composition were detected upon invasive transition of the tumoroids. The changes in EV protein cargo were counteracted by chemical inhibition of invasion. These results reveal the impact of the tumoroids’ invasive status on EV secretion and cargo, and highlight the necessity of in vivo‐mimicking conditions for uncovering novel cancer‐derived EV components.https://doi.org/10.1002/jex2.1243D culturesEV heterogeneityEV proteomeextracellular vesiclesinvasionPC3 cells |
| spellingShingle | Jens C. Luoto Leila S. Coelho‐Rato Cecilia Jungarå Sara H. Bengs Jannica Roininen John E. Eriksson Lea Sistonen Eva Henriksson Cancer cell invasion alters the protein profile of extracellular vesicles Journal of Extracellular Biology 3D cultures EV heterogeneity EV proteome extracellular vesicles invasion PC3 cells |
| title | Cancer cell invasion alters the protein profile of extracellular vesicles |
| title_full | Cancer cell invasion alters the protein profile of extracellular vesicles |
| title_fullStr | Cancer cell invasion alters the protein profile of extracellular vesicles |
| title_full_unstemmed | Cancer cell invasion alters the protein profile of extracellular vesicles |
| title_short | Cancer cell invasion alters the protein profile of extracellular vesicles |
| title_sort | cancer cell invasion alters the protein profile of extracellular vesicles |
| topic | 3D cultures EV heterogeneity EV proteome extracellular vesicles invasion PC3 cells |
| url | https://doi.org/10.1002/jex2.124 |
| work_keys_str_mv | AT jenscluoto cancercellinvasionalterstheproteinprofileofextracellularvesicles AT leilascoelhorato cancercellinvasionalterstheproteinprofileofextracellularvesicles AT ceciliajungara cancercellinvasionalterstheproteinprofileofextracellularvesicles AT sarahbengs cancercellinvasionalterstheproteinprofileofextracellularvesicles AT jannicaroininen cancercellinvasionalterstheproteinprofileofextracellularvesicles AT johneeriksson cancercellinvasionalterstheproteinprofileofextracellularvesicles AT leasistonen cancercellinvasionalterstheproteinprofileofextracellularvesicles AT evahenriksson cancercellinvasionalterstheproteinprofileofextracellularvesicles |