First Detection of <i>Ditylenchus destructor</i> Parasitizing Maize in Northeast China
Maize is one of the most important crops in the world. Heilongjiang province has the largest maize area in China. Plant-parasitic nematodes are important agricultural pests, which cause huge economic losses every year and have attracted global attention. Potato rot nematode <i>Ditylenchus dest...
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MDPI AG
2021-11-01
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| author | Fengjuan Pan Feng Li Yanzhi Mao Dan Liu Aoshuang Chen Dan Zhao Yanfeng Hu |
| author_facet | Fengjuan Pan Feng Li Yanzhi Mao Dan Liu Aoshuang Chen Dan Zhao Yanfeng Hu |
| author_sort | Fengjuan Pan |
| collection | DOAJ |
| description | Maize is one of the most important crops in the world. Heilongjiang province has the largest maize area in China. Plant-parasitic nematodes are important agricultural pests, which cause huge economic losses every year and have attracted global attention. Potato rot nematode <i>Ditylenchus destructor</i> is a plant-parasitic nematode with a wide range of hosts and strong survival ability in different environments, which brings risks to agricultural production. In 2020, <i>D. destructor</i> was detected in seven maize fields in Heilongjiang province. Morphological identification and molecular approach were used to characterize the isolated <i>D. destructor</i>. The observed morphological and morphometric characteristics were highly similar and consistent with the existing description. The DNA sequencing on the D2/D3 region of the ribosomal DNA 28S and the phylogenetic analysis showed that <i>D. destructor</i> population obtained from maize and other isolates infesting carrot, sweet potato, and potato were in subclade I supported by a 96% bootstrap value. Additionally, the phylogenetic analysis of the ITS rRNA gene sequence further indicated that this <i>D. destructor</i> population from maize clustered in a clade I group and belonged to ITS rRNA haplotype C. An inoculation experiment revealed that <i>D. destructor</i> was pathogenic on the maize seedlings in pots and caused the disease symptoms in the stem base of maize seedlings. This is the first report of <i>D. destructor</i> causing stem rot of maize in Heilongjiang province, and contributes additional information on disease control and safe production of maize in the region. |
| first_indexed | 2024-03-10T03:43:57Z |
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| id | doaj.art-76efd170a6c24bbc86e58e8033d9a532 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T03:43:57Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj.art-76efd170a6c24bbc86e58e8033d9a5322023-11-23T09:13:31ZengMDPI AGLife2075-17292021-11-011112130310.3390/life11121303First Detection of <i>Ditylenchus destructor</i> Parasitizing Maize in Northeast ChinaFengjuan Pan0Feng Li1Yanzhi Mao2Dan Liu3Aoshuang Chen4Dan Zhao5Yanfeng Hu6Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, ChinaSyngenta (China) Investment Co., Ltd., Beijing 102206, ChinaHeilongjiang Academy of Agricultural Sciences, Harbin 154026, ChinaSyngenta (China) Investment Co., Ltd., Beijing 102206, ChinaCollege of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150006, ChinaHeilongjiang Academy of Agricultural Sciences, Harbin 154026, ChinaNortheast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, ChinaMaize is one of the most important crops in the world. Heilongjiang province has the largest maize area in China. Plant-parasitic nematodes are important agricultural pests, which cause huge economic losses every year and have attracted global attention. Potato rot nematode <i>Ditylenchus destructor</i> is a plant-parasitic nematode with a wide range of hosts and strong survival ability in different environments, which brings risks to agricultural production. In 2020, <i>D. destructor</i> was detected in seven maize fields in Heilongjiang province. Morphological identification and molecular approach were used to characterize the isolated <i>D. destructor</i>. The observed morphological and morphometric characteristics were highly similar and consistent with the existing description. The DNA sequencing on the D2/D3 region of the ribosomal DNA 28S and the phylogenetic analysis showed that <i>D. destructor</i> population obtained from maize and other isolates infesting carrot, sweet potato, and potato were in subclade I supported by a 96% bootstrap value. Additionally, the phylogenetic analysis of the ITS rRNA gene sequence further indicated that this <i>D. destructor</i> population from maize clustered in a clade I group and belonged to ITS rRNA haplotype C. An inoculation experiment revealed that <i>D. destructor</i> was pathogenic on the maize seedlings in pots and caused the disease symptoms in the stem base of maize seedlings. This is the first report of <i>D. destructor</i> causing stem rot of maize in Heilongjiang province, and contributes additional information on disease control and safe production of maize in the region.https://www.mdpi.com/2075-1729/11/12/1303<i>Ditylenchus destructor</i>plant-parasitic nematodesmorphological characteristicmaize |
| spellingShingle | Fengjuan Pan Feng Li Yanzhi Mao Dan Liu Aoshuang Chen Dan Zhao Yanfeng Hu First Detection of <i>Ditylenchus destructor</i> Parasitizing Maize in Northeast China Life <i>Ditylenchus destructor</i> plant-parasitic nematodes morphological characteristic maize |
| title | First Detection of <i>Ditylenchus destructor</i> Parasitizing Maize in Northeast China |
| title_full | First Detection of <i>Ditylenchus destructor</i> Parasitizing Maize in Northeast China |
| title_fullStr | First Detection of <i>Ditylenchus destructor</i> Parasitizing Maize in Northeast China |
| title_full_unstemmed | First Detection of <i>Ditylenchus destructor</i> Parasitizing Maize in Northeast China |
| title_short | First Detection of <i>Ditylenchus destructor</i> Parasitizing Maize in Northeast China |
| title_sort | first detection of i ditylenchus destructor i parasitizing maize in northeast china |
| topic | <i>Ditylenchus destructor</i> plant-parasitic nematodes morphological characteristic maize |
| url | https://www.mdpi.com/2075-1729/11/12/1303 |
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