Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus
Acetyl-coenzyme A (CoA) is a key metabolite produced by the acetyl-CoA synthetase (ACS) gene in energy metabolism and biosynthetic pathways. ACS is speculated to be the branching site of monacolin K (MK) and citrinin production and related to the metabolite production of Monascus. In this study, the...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2022-12-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-202206-0002_effect-of-low-expression-level-of-acetyl-coenzyme-a-synthetase-gene-on-secondary-metabolite-in-monascus.php |
| _version_ | 1797899544302714880 |
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| author | Lin Lin Chundan Zhang Xinman Shao Pupin Liu Zhangfei Wu Yanxia Lu Hongzhen Guo |
| author_facet | Lin Lin Chundan Zhang Xinman Shao Pupin Liu Zhangfei Wu Yanxia Lu Hongzhen Guo |
| author_sort | Lin Lin |
| collection | DOAJ |
| description | Acetyl-coenzyme A (CoA) is a key metabolite produced by the acetyl-CoA synthetase (ACS) gene in energy metabolism and biosynthetic pathways. ACS is speculated to be the branching site of monacolin K (MK) and citrinin production and related to the metabolite production of Monascus. In this study, the ACS expression was inhibited by ribonucleic acid interference (RNAi). T7 was selected for a follow-up analysis of the lowest ACS expression, which was 0.401 times higher than that of the wild-type strain. The effects on the colony morphology of Monascus were determined. The morphological characteristics of mycelia and spores were observed under a scanning electron microscope. The contents of secondary metabolites, namely, MK and citrinin, were determined through high performance liquid chromatography (HPLC). Colour values were measured with a spectrophotometer. Results showed that the low ACS expression could inhibit the growth of Monascus colonies and the hypha and affect the formation and morphology of Monascus M1 spores. It could also inhibit the production of the main secondary metabolites, namely, MK, citrinin, and pigment. |
| first_indexed | 2024-04-10T08:31:38Z |
| format | Article |
| id | doaj.art-76f08e17a77b4346a620818895c20ed6 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:31:38Z |
| publishDate | 2022-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-76f08e17a77b4346a620818895c20ed62023-02-23T03:28:51ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172022-12-0140641442110.17221/4/2022-CJFScjf-202206-0002Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in MonascusLin LinChundan Zhang0Xinman Shao1Pupin Liu2Zhangfei Wu3Yanxia Lu4Hongzhen Guo5College of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, ChinaCollege of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, ChinaCollege of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, ChinaCollege of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, ChinaCollege of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, ChinaCollege of Life Sciences, Langfang Normal University, Langfang, China Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province, Langfang Normal University, Langfang, China Edible and Medicinal Fungi Research and Development Center of Hebei Universities, Langfang Normal University, Langfang, China Key Laboratory of Microbial Fermentation Research in Langfang, Langfang Normal University, Langfang, China Key Laboratory of Food Nutrition and Safety in Langfang, Langfang Normal University, Langfang, ChinaAcetyl-coenzyme A (CoA) is a key metabolite produced by the acetyl-CoA synthetase (ACS) gene in energy metabolism and biosynthetic pathways. ACS is speculated to be the branching site of monacolin K (MK) and citrinin production and related to the metabolite production of Monascus. In this study, the ACS expression was inhibited by ribonucleic acid interference (RNAi). T7 was selected for a follow-up analysis of the lowest ACS expression, which was 0.401 times higher than that of the wild-type strain. The effects on the colony morphology of Monascus were determined. The morphological characteristics of mycelia and spores were observed under a scanning electron microscope. The contents of secondary metabolites, namely, MK and citrinin, were determined through high performance liquid chromatography (HPLC). Colour values were measured with a spectrophotometer. Results showed that the low ACS expression could inhibit the growth of Monascus colonies and the hypha and affect the formation and morphology of Monascus M1 spores. It could also inhibit the production of the main secondary metabolites, namely, MK, citrinin, and pigment.https://cjfs.agriculturejournals.cz/artkey/cjf-202206-0002_effect-of-low-expression-level-of-acetyl-coenzyme-a-synthetase-gene-on-secondary-metabolite-in-monascus.phpcitrinincolonial morphologymonacolin kpigmentscanning electron microscope |
| spellingShingle | Lin Lin Chundan Zhang Xinman Shao Pupin Liu Zhangfei Wu Yanxia Lu Hongzhen Guo Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus Czech Journal of Food Sciences citrinin colonial morphology monacolin k pigment scanning electron microscope |
| title | Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus |
| title_full | Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus |
| title_fullStr | Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus |
| title_full_unstemmed | Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus |
| title_short | Effect of low expression level of acetyl coenzyme A synthetase gene on secondary metabolite in Monascus |
| title_sort | effect of low expression level of acetyl coenzyme a synthetase gene on secondary metabolite in monascus |
| topic | citrinin colonial morphology monacolin k pigment scanning electron microscope |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-202206-0002_effect-of-low-expression-level-of-acetyl-coenzyme-a-synthetase-gene-on-secondary-metabolite-in-monascus.php |
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