Interleukin-2, Interferon-gamma Gene Polymorphisms in Recurrent Aphthous Stomatitis
Recurrent aphthous stomatitis (RAS) is the most common oral ulcerative inflammatory disease with unknown etiology. IL-2 and IFN-γ are secreted by Th1 cells and the elevated levels of them have been reported in RAS. Single nucleotide polymorphisms (SNPs) of IL-2 and IFN-γ genes could alter the cytoki...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Karolinum Press
2017-09-01
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| Series: | Prague Medical Report |
| Subjects: | |
| Online Access: | https://pmr.lf1.cuni.cz/118/2/0081/ |
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| author | Shamsolmoulouk Najafi Hila Yousefi Mahsa Mohammadzadeh Alireza Zare Bidoki Elham Farhadi Nima Rezaei |
| author_facet | Shamsolmoulouk Najafi Hila Yousefi Mahsa Mohammadzadeh Alireza Zare Bidoki Elham Farhadi Nima Rezaei |
| author_sort | Shamsolmoulouk Najafi |
| collection | DOAJ |
| description | Recurrent aphthous stomatitis (RAS) is the most common oral ulcerative inflammatory disease with unknown etiology. IL-2 and IFN-γ are secreted by Th1 cells and the elevated levels of them have been reported in RAS. Single nucleotide polymorphisms (SNPs) of IL-2 and IFN-γ genes could alter the cytokine production. The aim of this study was to investigate frequencies of IL-2 and IFN-γ alleles and genotypes in a group of patients with minor-RAS (MiRAS). PCR-SSP method used to type genomic DNA of 64 Iranian patients with MiRAS for IL-2 gene (G –330 T) and (G +166 T) and IFN-γ gene at position UTR5644 (A/T). Frequency of each allele and genotype was compared with control group. IL-2 +166 G allele was significantly lower among patients which was reflected in significantly decreased of GG genotype at this position, while IL-2 +166 T allele was significantly higher among patients, IL-2 GT genotype was also significantly higher in RAS patients. No significant differences were found regarding IL-2 –330 G/T allele frequencies, while IL-2 GT genotype at this position was significantly higher among patients and IL-2 –330 TT genotype was significantly lower among RAS patients. Although no significant differences were found in IFN-γ allele frequencies at UTR5644 (A/T), AT genotype at this position was significantly overrepresented among patients compared with controls. Results of this study suggest that certain SNPs of IL-2 and IFN-γ genes have association with predisposition of individuals to RAS. More studies in different ethnic groups are needed to confirm results of this study. |
| first_indexed | 2024-12-11T00:06:05Z |
| format | Article |
| id | doaj.art-76f1ca8c2ba0461285440d03dfb9897d |
| institution | Directory Open Access Journal |
| issn | 1214-6994 2336-2936 |
| language | English |
| last_indexed | 2024-12-11T00:06:05Z |
| publishDate | 2017-09-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Prague Medical Report |
| spelling | doaj.art-76f1ca8c2ba0461285440d03dfb9897d2022-12-22T01:28:19ZengKarolinum PressPrague Medical Report1214-69942336-29362017-09-011182818610.14712/23362936.2017.75422Interleukin-2, Interferon-gamma Gene Polymorphisms in Recurrent Aphthous StomatitisShamsolmoulouk NajafiHila YousefiMahsa MohammadzadehAlireza Zare BidokiElham FarhadiNima RezaeiRecurrent aphthous stomatitis (RAS) is the most common oral ulcerative inflammatory disease with unknown etiology. IL-2 and IFN-γ are secreted by Th1 cells and the elevated levels of them have been reported in RAS. Single nucleotide polymorphisms (SNPs) of IL-2 and IFN-γ genes could alter the cytokine production. The aim of this study was to investigate frequencies of IL-2 and IFN-γ alleles and genotypes in a group of patients with minor-RAS (MiRAS). PCR-SSP method used to type genomic DNA of 64 Iranian patients with MiRAS for IL-2 gene (G –330 T) and (G +166 T) and IFN-γ gene at position UTR5644 (A/T). Frequency of each allele and genotype was compared with control group. IL-2 +166 G allele was significantly lower among patients which was reflected in significantly decreased of GG genotype at this position, while IL-2 +166 T allele was significantly higher among patients, IL-2 GT genotype was also significantly higher in RAS patients. No significant differences were found regarding IL-2 –330 G/T allele frequencies, while IL-2 GT genotype at this position was significantly higher among patients and IL-2 –330 TT genotype was significantly lower among RAS patients. Although no significant differences were found in IFN-γ allele frequencies at UTR5644 (A/T), AT genotype at this position was significantly overrepresented among patients compared with controls. Results of this study suggest that certain SNPs of IL-2 and IFN-γ genes have association with predisposition of individuals to RAS. More studies in different ethnic groups are needed to confirm results of this study.https://pmr.lf1.cuni.cz/118/2/0081/Recurrent aphthous stomatitisInterleukin-2Interferon-gammaSingle nucleotide polymorphisms |
| spellingShingle | Shamsolmoulouk Najafi Hila Yousefi Mahsa Mohammadzadeh Alireza Zare Bidoki Elham Farhadi Nima Rezaei Interleukin-2, Interferon-gamma Gene Polymorphisms in Recurrent Aphthous Stomatitis Prague Medical Report Recurrent aphthous stomatitis Interleukin-2 Interferon-gamma Single nucleotide polymorphisms |
| title | Interleukin-2, Interferon-gamma Gene Polymorphisms in Recurrent Aphthous Stomatitis |
| title_full | Interleukin-2, Interferon-gamma Gene Polymorphisms in Recurrent Aphthous Stomatitis |
| title_fullStr | Interleukin-2, Interferon-gamma Gene Polymorphisms in Recurrent Aphthous Stomatitis |
| title_full_unstemmed | Interleukin-2, Interferon-gamma Gene Polymorphisms in Recurrent Aphthous Stomatitis |
| title_short | Interleukin-2, Interferon-gamma Gene Polymorphisms in Recurrent Aphthous Stomatitis |
| title_sort | interleukin 2 interferon gamma gene polymorphisms in recurrent aphthous stomatitis |
| topic | Recurrent aphthous stomatitis Interleukin-2 Interferon-gamma Single nucleotide polymorphisms |
| url | https://pmr.lf1.cuni.cz/118/2/0081/ |
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