Identification of 14-3-3-like protein in sugarcane (Saccharum officinarum)
In a search of the sugarcane expressed sequence tag (SUCEST) database we located three full-length cDNAs (SCCCLR1022D05.g, SCCCRZ1001D02.g and SCBFLR1026E02.g) encoding the 14-3-3 proteins from sugarcane (Saccharum officinarum). The encoded proteins were identified based on the clustering of the exp...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2001-12-01
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| Series: | Genetics and Molecular Biology |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100007 |
| Summary: | In a search of the sugarcane expressed sequence tag (SUCEST) database we located three full-length cDNAs (SCCCLR1022D05.g, SCCCRZ1001D02.g and SCBFLR1026E02.g) encoding the 14-3-3 proteins from sugarcane (Saccharum officinarum). The encoded proteins were identified based on the clustering of the expressed sequence tags and were shown to encode proteins similar to 14-3-3 proteins of other monocotyledonous plants. Cluster SCCCLR1022D05.g was 99% similar to the maize 14-3-3-like protein (gi|1345587) while cluster SCCCRZ1001D02.g shared 96% and SCBFLR1026E02.g 94% similarity with the 14-3-3 protein of rice (gi|7435022). Although 14-3-3 proteins have been reported to be specific to particular species, tissue or organ from which they were isolated, all three sugarcane clusters were found to be expressed in several tissues.<br>Seqüências completas de três cDNAs que codificam proteínas 14-3-3 de cana-de-açúcar (Saccharum officinarum) foram encontradas no projeto genoma EST de cana-de-açúcar. As proteínas codificadas por essas seqüências foram identificadas baseando-se no agrupamento de ESTs de cana-de-açúcar e, três "clusters" (SCCCLR1022 D05.g, SCCCRZ1001D02.g e SCBFLR1026E02.g) foram similares a proteínas 14-3-3 de outras monocotiledôneas. O cluster SCCCLR1022D05.g apresentou similaridade de 99% com a proteína 14-3-3 de milho (gi|1345587) e, os clusters SCCCRZ1001D02.g e SCBFLR1026E02.g foram similares a proteína 14-3-3 de arroz (gi|7435022), 96% e 94%, respectivamente. Embora proteínas 14-3-3 têm sido relatadas como sendo específicas a organismos, tecidos e órgãos, todos os "clusters" de cana-de-açúcar correspondentes a 14-3-3 foram provenientes de cDNAs advindos de diferentes tecidos. |
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| ISSN: | 1415-4757 1678-4685 |