Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study
Microbes capable of metabolizing gluten are common in various parts of the intestinal tract. In this study, saliva and fecal samples were obtained from 10 adolescents (13–18 years of age), five of which had celiac disease (CD) and five of which were healthy volunteers (HV). Culture-enriched saliva a...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2375 |
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| author | Tilen Senicar Andraz Kukovicic Valerija Tkalec Aleksander Mahnic Jernej Dolinsek Maja Rupnik |
| author_facet | Tilen Senicar Andraz Kukovicic Valerija Tkalec Aleksander Mahnic Jernej Dolinsek Maja Rupnik |
| author_sort | Tilen Senicar |
| collection | DOAJ |
| description | Microbes capable of metabolizing gluten are common in various parts of the intestinal tract. In this study, saliva and fecal samples were obtained from 10 adolescents (13–18 years of age), five of which had celiac disease (CD) and five of which were healthy volunteers (HV). Culture-enriched saliva and fecal samples were compared with molecular profiling, and microorganisms displaying lysis zones on gluten-containing media (i.e., gluten-degrading microorganisms; GDMs) were isolated. In total, 45 gluten-degrading strains were isolated, belonging to 13 genera and 15 species, including <i>Candida albicans</i> and <i>Veillonella</i>. GDMs were more common in HVs compared to CD patients and more diverse in saliva compared to feces. In saliva, GDMs showed partial overlap between HVs and CD patients. Bacterial communities in fecal samples determined with amplicon sequencing significantly differed between CD patients and HVs. Overall, 7–46 of all operational taxonomic units (OTUs) per sample were below the detection limit in the fecal samples but were present in the cultivated samples, and mainly included representatives from <i>Lactobacillus</i> and <i>Enterococcus</i>. Furthermore, differences in fecal short-chain fatty-acid concentrations between CD patients and HVs, as well as their correlations with bacterial taxa, were demonstrated. |
| first_indexed | 2024-03-10T05:14:49Z |
| format | Article |
| id | doaj.art-76f2ba18e9a3417f85f5a75f189224cd |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:14:49Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-76f2ba18e9a3417f85f5a75f189224cd2023-11-23T00:30:31ZengMDPI AGMicroorganisms2076-26072021-11-01911237510.3390/microorganisms9112375Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory StudyTilen Senicar0Andraz Kukovicic1Valerija Tkalec2Aleksander Mahnic3Jernej Dolinsek4Maja Rupnik5Faculty of Medicine, University of Maribor, 2000 Maribor, SloveniaFaculty of Medicine, University of Maribor, 2000 Maribor, SloveniaFaculty of Medicine, University of Maribor, 2000 Maribor, SloveniaFaculty of Medicine, University of Maribor, 2000 Maribor, SloveniaDepartment of Paediatrics, University Clinical Centre Maribor, 2000 Maribor, SloveniaFaculty of Medicine, University of Maribor, 2000 Maribor, SloveniaMicrobes capable of metabolizing gluten are common in various parts of the intestinal tract. In this study, saliva and fecal samples were obtained from 10 adolescents (13–18 years of age), five of which had celiac disease (CD) and five of which were healthy volunteers (HV). Culture-enriched saliva and fecal samples were compared with molecular profiling, and microorganisms displaying lysis zones on gluten-containing media (i.e., gluten-degrading microorganisms; GDMs) were isolated. In total, 45 gluten-degrading strains were isolated, belonging to 13 genera and 15 species, including <i>Candida albicans</i> and <i>Veillonella</i>. GDMs were more common in HVs compared to CD patients and more diverse in saliva compared to feces. In saliva, GDMs showed partial overlap between HVs and CD patients. Bacterial communities in fecal samples determined with amplicon sequencing significantly differed between CD patients and HVs. Overall, 7–46 of all operational taxonomic units (OTUs) per sample were below the detection limit in the fecal samples but were present in the cultivated samples, and mainly included representatives from <i>Lactobacillus</i> and <i>Enterococcus</i>. Furthermore, differences in fecal short-chain fatty-acid concentrations between CD patients and HVs, as well as their correlations with bacterial taxa, were demonstrated.https://www.mdpi.com/2076-2607/9/11/2375gluten-degrading microorganisms<i>Veillonella</i><i>Candida</i>short-chain fatty acidsceliac diseasemicrobiota |
| spellingShingle | Tilen Senicar Andraz Kukovicic Valerija Tkalec Aleksander Mahnic Jernej Dolinsek Maja Rupnik Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study Microorganisms gluten-degrading microorganisms <i>Veillonella</i> <i>Candida</i> short-chain fatty acids celiac disease microbiota |
| title | Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study |
| title_full | Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study |
| title_fullStr | Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study |
| title_full_unstemmed | Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study |
| title_short | Comparison of Microbial Populations in Saliva and Feces from Healthy and Celiac Adolescents with Conventional and Molecular Approaches after Cultivation on Gluten-Containing Media: An Exploratory Study |
| title_sort | comparison of microbial populations in saliva and feces from healthy and celiac adolescents with conventional and molecular approaches after cultivation on gluten containing media an exploratory study |
| topic | gluten-degrading microorganisms <i>Veillonella</i> <i>Candida</i> short-chain fatty acids celiac disease microbiota |
| url | https://www.mdpi.com/2076-2607/9/11/2375 |
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