ENZYMATIC HYDROLYSIS OF SWITCHGRASS AND COASTAL BERMUDA GRASS PRETREATED USING DIFFERENT CHEMICAL METHODS
To investigate the effects of biomass feedstock and pretreatment method on the enzyme requirement during hydrolysis, swichgrass and coastal Bermuda grass pretreated using H2SO4, NaOH, and Ca(OH)2 at the optimal conditions were subjected to enzymatic hydrolysis using two enzyme combinations: NS 50013...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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North Carolina State University
2011-06-01
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| Series: | BioResources |
| Subjects: | |
| Online Access: | http://www.ncsu.edu/bioresources/BioRes_06/BioRes_06_3_2990_Xu_WSC_Enzym_Hydrol_Switchgrass_Chem_Methods_1529.pdf |
| _version_ | 1828422120131002368 |
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| author | Jiele Xu Ziyu Wang Ratna Sharma-Shivappa Jay Cheng |
| author_facet | Jiele Xu Ziyu Wang Ratna Sharma-Shivappa Jay Cheng |
| author_sort | Jiele Xu |
| collection | DOAJ |
| description | To investigate the effects of biomass feedstock and pretreatment method on the enzyme requirement during hydrolysis, swichgrass and coastal Bermuda grass pretreated using H2SO4, NaOH, and Ca(OH)2 at the optimal conditions were subjected to enzymatic hydrolysis using two enzyme combinations: NS 50013 + NS 50010 and Cellic CTec + Cellic HTec. The enzyme loadings were optimized, and correlations between feedstock property, pretreatment strategy, and enzyme usage were evaluated. The results show that pretreatment methods resulting in greater lignin contents in the pretreated biomass were generally associated with higher enzyme requirements. More sugars could be recovered from alkaline-pretreated biomass during enzymatic hydrolysis due to the better carbohydrate preservation achieved at mild pretreatment temperatures. The cellulase enzyme, Cellic CTec, was more efficient in catalyzing the hydrolysis of coastal Bermuda grass, a feedstock more digestible than the pretreated swichgrass, following pretreatment with NaOH or Ca(OH)2. |
| first_indexed | 2024-12-10T15:44:07Z |
| format | Article |
| id | doaj.art-76f2efcda44641f7ac2a31d63747d930 |
| institution | Directory Open Access Journal |
| issn | 1930-2126 |
| language | English |
| last_indexed | 2024-12-10T15:44:07Z |
| publishDate | 2011-06-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-76f2efcda44641f7ac2a31d63747d9302022-12-22T01:43:02ZengNorth Carolina State UniversityBioResources1930-21262011-06-016329903003ENZYMATIC HYDROLYSIS OF SWITCHGRASS AND COASTAL BERMUDA GRASS PRETREATED USING DIFFERENT CHEMICAL METHODSJiele XuZiyu WangRatna Sharma-ShivappaJay ChengTo investigate the effects of biomass feedstock and pretreatment method on the enzyme requirement during hydrolysis, swichgrass and coastal Bermuda grass pretreated using H2SO4, NaOH, and Ca(OH)2 at the optimal conditions were subjected to enzymatic hydrolysis using two enzyme combinations: NS 50013 + NS 50010 and Cellic CTec + Cellic HTec. The enzyme loadings were optimized, and correlations between feedstock property, pretreatment strategy, and enzyme usage were evaluated. The results show that pretreatment methods resulting in greater lignin contents in the pretreated biomass were generally associated with higher enzyme requirements. More sugars could be recovered from alkaline-pretreated biomass during enzymatic hydrolysis due to the better carbohydrate preservation achieved at mild pretreatment temperatures. The cellulase enzyme, Cellic CTec, was more efficient in catalyzing the hydrolysis of coastal Bermuda grass, a feedstock more digestible than the pretreated swichgrass, following pretreatment with NaOH or Ca(OH)2.www.ncsu.edu/bioresources/BioRes_06/BioRes_06_3_2990_Xu_WSC_Enzym_Hydrol_Switchgrass_Chem_Methods_1529.pdfBiomass conversionCellulase enzymeChemical pretreatmentCoastal Bermuda grassEnzymatic hydrolysisEthanolSwitchgrass |
| spellingShingle | Jiele Xu Ziyu Wang Ratna Sharma-Shivappa Jay Cheng ENZYMATIC HYDROLYSIS OF SWITCHGRASS AND COASTAL BERMUDA GRASS PRETREATED USING DIFFERENT CHEMICAL METHODS BioResources Biomass conversion Cellulase enzyme Chemical pretreatment Coastal Bermuda grass Enzymatic hydrolysis Ethanol Switchgrass |
| title | ENZYMATIC HYDROLYSIS OF SWITCHGRASS AND COASTAL BERMUDA GRASS PRETREATED USING DIFFERENT CHEMICAL METHODS |
| title_full | ENZYMATIC HYDROLYSIS OF SWITCHGRASS AND COASTAL BERMUDA GRASS PRETREATED USING DIFFERENT CHEMICAL METHODS |
| title_fullStr | ENZYMATIC HYDROLYSIS OF SWITCHGRASS AND COASTAL BERMUDA GRASS PRETREATED USING DIFFERENT CHEMICAL METHODS |
| title_full_unstemmed | ENZYMATIC HYDROLYSIS OF SWITCHGRASS AND COASTAL BERMUDA GRASS PRETREATED USING DIFFERENT CHEMICAL METHODS |
| title_short | ENZYMATIC HYDROLYSIS OF SWITCHGRASS AND COASTAL BERMUDA GRASS PRETREATED USING DIFFERENT CHEMICAL METHODS |
| title_sort | enzymatic hydrolysis of switchgrass and coastal bermuda grass pretreated using different chemical methods |
| topic | Biomass conversion Cellulase enzyme Chemical pretreatment Coastal Bermuda grass Enzymatic hydrolysis Ethanol Switchgrass |
| url | http://www.ncsu.edu/bioresources/BioRes_06/BioRes_06_3_2990_Xu_WSC_Enzym_Hydrol_Switchgrass_Chem_Methods_1529.pdf |
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