Selenium nanoparticles induce suppressed function of tumor associated macrophages and inhibit Dalton's lymphoma proliferation
Selenium Nanoparticle (SeNPs) is reported that it enhances and maintains optimal immune during infection and malignancies. To this end, we examined the role of selenium on TAMS whose anti-tumor function suppressed which favor tumor progression. BALB/c (H2d) strain of mice non-Hodgkin type of Dalton&...
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Elsevier
2017-12-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580817301292 |
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| author | Pramod Kumar Gautam Sanjay Kumar M.S. Tomar Rishi Kant Singh A. Acharya Sanjay Kumar B. Ram |
| author_facet | Pramod Kumar Gautam Sanjay Kumar M.S. Tomar Rishi Kant Singh A. Acharya Sanjay Kumar B. Ram |
| author_sort | Pramod Kumar Gautam |
| collection | DOAJ |
| description | Selenium Nanoparticle (SeNPs) is reported that it enhances and maintains optimal immune during infection and malignancies. To this end, we examined the role of selenium on TAMS whose anti-tumor function suppressed which favor tumor progression. BALB/c (H2d) strain of mice non-Hodgkin type of Dalton's cell line was used to check the role of carboxlic group induced, synthesized SeNPs on TAMs. Screening of IC50 value was done primarily trypen blue exclusion assay and 50% proliferation of DL cells inhibited 40 ng/ml to 50 ng/. Treatment also decreases ΔΨm, fragmentation of DNA of DL cells and arrest cells cycle in G1/G0 phage. Untreated TAMs cells showing suppressed expression of ROS, adhesion, phagocytosis, fusion and receptor profiling such as ICAM-1, CD47, CD172α. Which was induced more as compare to untreated group. SeNPs have potential to induce the anti-tumor function of TAMs whose anti-tumor function down-regulated pliable shifted towards tumor progression. It decreased the proliferation of DL cell by inducing apoptosis. Therefore, the synthesized SeNPs could be used for imaging diagnosis and cancer therapy which must be cost effective with negligible side effects shifted towards tumor progression. It decreased the proliferation of DL cell by inducing apoptosis. |
| first_indexed | 2024-12-12T20:00:22Z |
| format | Article |
| id | doaj.art-76f781024bda43ff869b186a6ead69a1 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-12T20:00:22Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
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| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-76f781024bda43ff869b186a6ead69a12022-12-22T00:13:46ZengElsevierBiochemistry and Biophysics Reports2405-58082017-12-0112C17218410.1016/j.bbrep.2017.09.005Selenium nanoparticles induce suppressed function of tumor associated macrophages and inhibit Dalton's lymphoma proliferationPramod Kumar Gautam0Sanjay Kumar1M.S. Tomar2Rishi Kant Singh3A. Acharya4Sanjay Kumar5B. Ram6Institute of Science, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi, IndiaInstitute of Science, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi, IndiaInstitute of Science, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi, IndiaInstitute of Science, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi, IndiaInstitute of Science, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi, IndiaDepartment of Drayaguna, Faculty of Ayurveda, Institute of Medical Science, Banaras Hindu University, Varanasi, IndiaDepartment of Drayaguna, Faculty of Ayurveda, Institute of Medical Science, Banaras Hindu University, Varanasi, IndiaSelenium Nanoparticle (SeNPs) is reported that it enhances and maintains optimal immune during infection and malignancies. To this end, we examined the role of selenium on TAMS whose anti-tumor function suppressed which favor tumor progression. BALB/c (H2d) strain of mice non-Hodgkin type of Dalton's cell line was used to check the role of carboxlic group induced, synthesized SeNPs on TAMs. Screening of IC50 value was done primarily trypen blue exclusion assay and 50% proliferation of DL cells inhibited 40 ng/ml to 50 ng/. Treatment also decreases ΔΨm, fragmentation of DNA of DL cells and arrest cells cycle in G1/G0 phage. Untreated TAMs cells showing suppressed expression of ROS, adhesion, phagocytosis, fusion and receptor profiling such as ICAM-1, CD47, CD172α. Which was induced more as compare to untreated group. SeNPs have potential to induce the anti-tumor function of TAMs whose anti-tumor function down-regulated pliable shifted towards tumor progression. It decreased the proliferation of DL cell by inducing apoptosis. Therefore, the synthesized SeNPs could be used for imaging diagnosis and cancer therapy which must be cost effective with negligible side effects shifted towards tumor progression. It decreased the proliferation of DL cell by inducing apoptosis.http://www.sciencedirect.com/science/article/pii/S2405580817301292Selenium nanoparticleTAMsLymphomaROSAnti-tumor function |
| spellingShingle | Pramod Kumar Gautam Sanjay Kumar M.S. Tomar Rishi Kant Singh A. Acharya Sanjay Kumar B. Ram Selenium nanoparticles induce suppressed function of tumor associated macrophages and inhibit Dalton's lymphoma proliferation Biochemistry and Biophysics Reports Selenium nanoparticle TAMs Lymphoma ROS Anti-tumor function |
| title | Selenium nanoparticles induce suppressed function of tumor associated macrophages and inhibit Dalton's lymphoma proliferation |
| title_full | Selenium nanoparticles induce suppressed function of tumor associated macrophages and inhibit Dalton's lymphoma proliferation |
| title_fullStr | Selenium nanoparticles induce suppressed function of tumor associated macrophages and inhibit Dalton's lymphoma proliferation |
| title_full_unstemmed | Selenium nanoparticles induce suppressed function of tumor associated macrophages and inhibit Dalton's lymphoma proliferation |
| title_short | Selenium nanoparticles induce suppressed function of tumor associated macrophages and inhibit Dalton's lymphoma proliferation |
| title_sort | selenium nanoparticles induce suppressed function of tumor associated macrophages and inhibit dalton s lymphoma proliferation |
| topic | Selenium nanoparticle TAMs Lymphoma ROS Anti-tumor function |
| url | http://www.sciencedirect.com/science/article/pii/S2405580817301292 |
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