TaMCA4, a Novel Wheat Metacaspase Gene Functions in Programmed Cell Death Induced by the Fungal Pathogen Puccinia striiformis f. sp. tritici
Programmed cell death (PCD) is a physiological process to remove redundant or harmful cells, for the development of multicellular organisms, or for restricting the spread of pathogens (hypersensitive response). Metacaspases are cysteine-dependent proteases which play an essential role in PCD. Tritic...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2012-06-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-11-11-0283-R |
| _version_ | 1811261188622778368 |
|---|---|
| author | Xiaodong Wang Xiaojie Wang Hao Feng Chunlei Tang Pengfei Bai Guorong Wei Lili Huang Zhensheng Kang |
| author_facet | Xiaodong Wang Xiaojie Wang Hao Feng Chunlei Tang Pengfei Bai Guorong Wei Lili Huang Zhensheng Kang |
| author_sort | Xiaodong Wang |
| collection | DOAJ |
| description | Programmed cell death (PCD) is a physiological process to remove redundant or harmful cells, for the development of multicellular organisms, or for restricting the spread of pathogens (hypersensitive response). Metacaspases are cysteine-dependent proteases which play an essential role in PCD. Triticum aestivum metacaspase 4 (TaMCA4) is a type II metacaspase gene cloned from ‘Suwon11’ wheat, with typical structural features such as peptidase C14 caspase domain and a long linker sequence between the two subunits. Transient expression of TaMCA4 in tobacco leaves failed to induce PCD directly but enhanced cell death triggered by a mouse Bax gene or a candidate effector gene from Puccinia striiformis f. sp. tritici. Enhancement of PCD was also observed in wheat leaves co-bombarded with TaMCA4. When challenged with the avirulent race of P. striiformis f. sp. tritici, the expression level of TaMCA4 in wheat leaves was sharply upregulated, whereas the transcript level was not significantly induced by the virulent race. Moreover, knocking down TaMCA4 expression by virus-induced gene silencing enhanced the susceptibility of Suwon11 to the avirulent race of P. striiformis f. sp. tritici and reduced the necrotic area at infection sites. |
| first_indexed | 2024-04-12T18:59:40Z |
| format | Article |
| id | doaj.art-76fb6781cb544c16962f163de063acd6 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T18:59:40Z |
| publishDate | 2012-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-76fb6781cb544c16962f163de063acd62022-12-22T03:20:12ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062012-06-0125675576410.1094/MPMI-11-11-0283-RTaMCA4, a Novel Wheat Metacaspase Gene Functions in Programmed Cell Death Induced by the Fungal Pathogen Puccinia striiformis f. sp. triticiXiaodong WangXiaojie WangHao FengChunlei TangPengfei BaiGuorong WeiLili HuangZhensheng KangProgrammed cell death (PCD) is a physiological process to remove redundant or harmful cells, for the development of multicellular organisms, or for restricting the spread of pathogens (hypersensitive response). Metacaspases are cysteine-dependent proteases which play an essential role in PCD. Triticum aestivum metacaspase 4 (TaMCA4) is a type II metacaspase gene cloned from ‘Suwon11’ wheat, with typical structural features such as peptidase C14 caspase domain and a long linker sequence between the two subunits. Transient expression of TaMCA4 in tobacco leaves failed to induce PCD directly but enhanced cell death triggered by a mouse Bax gene or a candidate effector gene from Puccinia striiformis f. sp. tritici. Enhancement of PCD was also observed in wheat leaves co-bombarded with TaMCA4. When challenged with the avirulent race of P. striiformis f. sp. tritici, the expression level of TaMCA4 in wheat leaves was sharply upregulated, whereas the transcript level was not significantly induced by the virulent race. Moreover, knocking down TaMCA4 expression by virus-induced gene silencing enhanced the susceptibility of Suwon11 to the avirulent race of P. striiformis f. sp. tritici and reduced the necrotic area at infection sites.https://apsjournals.apsnet.org/doi/10.1094/MPMI-11-11-0283-R |
| spellingShingle | Xiaodong Wang Xiaojie Wang Hao Feng Chunlei Tang Pengfei Bai Guorong Wei Lili Huang Zhensheng Kang TaMCA4, a Novel Wheat Metacaspase Gene Functions in Programmed Cell Death Induced by the Fungal Pathogen Puccinia striiformis f. sp. tritici Molecular Plant-Microbe Interactions |
| title | TaMCA4, a Novel Wheat Metacaspase Gene Functions in Programmed Cell Death Induced by the Fungal Pathogen Puccinia striiformis f. sp. tritici |
| title_full | TaMCA4, a Novel Wheat Metacaspase Gene Functions in Programmed Cell Death Induced by the Fungal Pathogen Puccinia striiformis f. sp. tritici |
| title_fullStr | TaMCA4, a Novel Wheat Metacaspase Gene Functions in Programmed Cell Death Induced by the Fungal Pathogen Puccinia striiformis f. sp. tritici |
| title_full_unstemmed | TaMCA4, a Novel Wheat Metacaspase Gene Functions in Programmed Cell Death Induced by the Fungal Pathogen Puccinia striiformis f. sp. tritici |
| title_short | TaMCA4, a Novel Wheat Metacaspase Gene Functions in Programmed Cell Death Induced by the Fungal Pathogen Puccinia striiformis f. sp. tritici |
| title_sort | tamca4 a novel wheat metacaspase gene functions in programmed cell death induced by the fungal pathogen puccinia striiformis f sp tritici |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-11-11-0283-R |
| work_keys_str_mv | AT xiaodongwang tamca4anovelwheatmetacaspasegenefunctionsinprogrammedcelldeathinducedbythefungalpathogenpucciniastriiformisfsptritici AT xiaojiewang tamca4anovelwheatmetacaspasegenefunctionsinprogrammedcelldeathinducedbythefungalpathogenpucciniastriiformisfsptritici AT haofeng tamca4anovelwheatmetacaspasegenefunctionsinprogrammedcelldeathinducedbythefungalpathogenpucciniastriiformisfsptritici AT chunleitang tamca4anovelwheatmetacaspasegenefunctionsinprogrammedcelldeathinducedbythefungalpathogenpucciniastriiformisfsptritici AT pengfeibai tamca4anovelwheatmetacaspasegenefunctionsinprogrammedcelldeathinducedbythefungalpathogenpucciniastriiformisfsptritici AT guorongwei tamca4anovelwheatmetacaspasegenefunctionsinprogrammedcelldeathinducedbythefungalpathogenpucciniastriiformisfsptritici AT lilihuang tamca4anovelwheatmetacaspasegenefunctionsinprogrammedcelldeathinducedbythefungalpathogenpucciniastriiformisfsptritici AT zhenshengkang tamca4anovelwheatmetacaspasegenefunctionsinprogrammedcelldeathinducedbythefungalpathogenpucciniastriiformisfsptritici |