Olea Europaea Geminivirus: A Novel Bipartite Geminivirid Infecting Olive Trees
In 2014, high-throughput sequencing of libraries of total DNA from olive trees allowed the identification of two geminivirus-like contigs. After conventional resequencing of the two genomic DNAs, their analysis revealed they belonged to the same viral entity, for which the provisional name of Olea e...
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MDPI AG
2021-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/3/481 |
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| author | Michela Chiumenti Claudia Greco Angelo De Stradis Giuliana Loconsole Vincenzo Cavalieri Giuseppe Altamura Stefania Zicca Pasquale Saldarelli Maria Saponari |
| author_facet | Michela Chiumenti Claudia Greco Angelo De Stradis Giuliana Loconsole Vincenzo Cavalieri Giuseppe Altamura Stefania Zicca Pasquale Saldarelli Maria Saponari |
| author_sort | Michela Chiumenti |
| collection | DOAJ |
| description | In 2014, high-throughput sequencing of libraries of total DNA from olive trees allowed the identification of two geminivirus-like contigs. After conventional resequencing of the two genomic DNAs, their analysis revealed they belonged to the same viral entity, for which the provisional name of Olea europaea geminivirus (OEGV) was proposed. Although DNA-A showed a genome organization similar to that of New World begomoviruses, DNA-B had a peculiar ORF arrangement, consisting of a movement protein (MP) in the virion sense and a protein with unknown function on the complementary sense. Phylogenetic analysis performed either on full-length genome or on coat protein, replication associated protein (Rep), and MP sequences did not endorse the inclusion of this virus in any of the established genera in the family <i>Geminiviridae</i>. A survey of 55 plants revealed that the virus is widespread in Apulia (Italy) with 91% of the samples testing positive, although no correlation of OEGV with a disease or specific symptoms was encountered. Southern blot assay suggested that the virus is not integrated in the olive genome. The study of OEGV-derived siRNA obtained from small RNA libraries of leaves and fruits of three different cultivars, showed that the accumulation of the two genomic components is influenced by the plant genotype while virus-derived-siRNA profile is in line with other geminivirids reported in literature. Single-nucleotide polymorphism (SNP) analysis unveiled a low intra-specific variability. |
| first_indexed | 2024-03-10T13:13:33Z |
| format | Article |
| id | doaj.art-76ffd8aff1fd46429e3648df9e320a45 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T13:13:33Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-76ffd8aff1fd46429e3648df9e320a452023-11-21T10:34:34ZengMDPI AGViruses1999-49152021-03-0113348110.3390/v13030481Olea Europaea Geminivirus: A Novel Bipartite Geminivirid Infecting Olive TreesMichela Chiumenti0Claudia Greco1Angelo De Stradis2Giuliana Loconsole3Vincenzo Cavalieri4Giuseppe Altamura5Stefania Zicca6Pasquale Saldarelli7Maria Saponari8Institute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, ItalyInstitute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, ItalyInstitute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, ItalyInstitute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, ItalyInstitute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, ItalyInstitute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, ItalyInstitute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, ItalyInstitute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, ItalyInstitute for Sustainable Plant Protection, CNR, Via Amendola 122/D, 70126 Bari, ItalyIn 2014, high-throughput sequencing of libraries of total DNA from olive trees allowed the identification of two geminivirus-like contigs. After conventional resequencing of the two genomic DNAs, their analysis revealed they belonged to the same viral entity, for which the provisional name of Olea europaea geminivirus (OEGV) was proposed. Although DNA-A showed a genome organization similar to that of New World begomoviruses, DNA-B had a peculiar ORF arrangement, consisting of a movement protein (MP) in the virion sense and a protein with unknown function on the complementary sense. Phylogenetic analysis performed either on full-length genome or on coat protein, replication associated protein (Rep), and MP sequences did not endorse the inclusion of this virus in any of the established genera in the family <i>Geminiviridae</i>. A survey of 55 plants revealed that the virus is widespread in Apulia (Italy) with 91% of the samples testing positive, although no correlation of OEGV with a disease or specific symptoms was encountered. Southern blot assay suggested that the virus is not integrated in the olive genome. The study of OEGV-derived siRNA obtained from small RNA libraries of leaves and fruits of three different cultivars, showed that the accumulation of the two genomic components is influenced by the plant genotype while virus-derived-siRNA profile is in line with other geminivirids reported in literature. Single-nucleotide polymorphism (SNP) analysis unveiled a low intra-specific variability.https://www.mdpi.com/1999-4915/13/3/481high-throughput sequencingolive treestotal DNA library<i>Geminiviridae</i>small RNAssDNA-virus |
| spellingShingle | Michela Chiumenti Claudia Greco Angelo De Stradis Giuliana Loconsole Vincenzo Cavalieri Giuseppe Altamura Stefania Zicca Pasquale Saldarelli Maria Saponari Olea Europaea Geminivirus: A Novel Bipartite Geminivirid Infecting Olive Trees Viruses high-throughput sequencing olive trees total DNA library <i>Geminiviridae</i> small RNA ssDNA-virus |
| title | Olea Europaea Geminivirus: A Novel Bipartite Geminivirid Infecting Olive Trees |
| title_full | Olea Europaea Geminivirus: A Novel Bipartite Geminivirid Infecting Olive Trees |
| title_fullStr | Olea Europaea Geminivirus: A Novel Bipartite Geminivirid Infecting Olive Trees |
| title_full_unstemmed | Olea Europaea Geminivirus: A Novel Bipartite Geminivirid Infecting Olive Trees |
| title_short | Olea Europaea Geminivirus: A Novel Bipartite Geminivirid Infecting Olive Trees |
| title_sort | olea europaea geminivirus a novel bipartite geminivirid infecting olive trees |
| topic | high-throughput sequencing olive trees total DNA library <i>Geminiviridae</i> small RNA ssDNA-virus |
| url | https://www.mdpi.com/1999-4915/13/3/481 |
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