Influence of Supplementation of Lactoferrin, Melittin and Cecropin A to Rat Diet on Changes in Faecal Ammonia Concentrations, Short-Chain Fatty Acid Concentrations and Activities of Bacterial Enzymes
We hypothesised that the dietary addition of the bioactive antimicrobial protein lactoferrin (LF) and peptides melittin (MT) or cecropin A (CR) at a dosage of 100 mg/kg to the diet of Wistar rats would result in strong modulatory effects on faecal microbial enzymatic activity, short-chain fatty acid...
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MDPI AG
2021-04-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/5/1203 |
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| author | Jerzy Juśkiewicz Aleksandra Rawicka Bartosz Fotschki Michał Majewski Zenon Zduńczyk |
| author_facet | Jerzy Juśkiewicz Aleksandra Rawicka Bartosz Fotschki Michał Majewski Zenon Zduńczyk |
| author_sort | Jerzy Juśkiewicz |
| collection | DOAJ |
| description | We hypothesised that the dietary addition of the bioactive antimicrobial protein lactoferrin (LF) and peptides melittin (MT) or cecropin A (CR) at a dosage of 100 mg/kg to the diet of Wistar rats would result in strong modulatory effects on faecal microbial enzymatic activity, short-chain fatty acid and ammonia concentrations. To date, the changes in bacterial extracellular and intracellular enzymatic activities upon addition of dietary AMPs have not yet been studied. This experiment lasted 15 days; during the first 5 day period, the rats were fed the control diet (S) and diets supplemented with LF, MT or CR. On days 6–15, all rats were fed the control S diet. The faecal fermentation processes were substantially stopped after two days of treatment, on average, in all rats receiving LF and two AMPs. The deepest suppression effect was observed on the last day of treatment (day 5) and persisted through days 5–8. The highest decreases in faecal bacterial β-glucosidase and β-glucuronidase activities as well as in SCFA and ammonia concentrations were observed in the rats fed the CR diet. Only in the CR animals did the mechanism of suppressed microbial fermentation involve diminished enzyme release from bacterial cells to the digesta. |
| first_indexed | 2024-03-10T12:04:46Z |
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| id | doaj.art-770048c7d3e34f89acdea9edbddfdb72 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T12:04:46Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-770048c7d3e34f89acdea9edbddfdb722023-11-21T16:40:34ZengMDPI AGAnimals2076-26152021-04-01115120310.3390/ani11051203Influence of Supplementation of Lactoferrin, Melittin and Cecropin A to Rat Diet on Changes in Faecal Ammonia Concentrations, Short-Chain Fatty Acid Concentrations and Activities of Bacterial EnzymesJerzy Juśkiewicz0Aleksandra Rawicka1Bartosz Fotschki2Michał Majewski3Zenon Zduńczyk4Institute of Animal Reproduction and Food Research, Department of Biological Function of Food, Polish Academy of Sciences, 10-748 Olsztyn, PolandPoliclinico Veterinario Roma Sud, Ophthalmology, 00173 Rome, ItalyInstitute of Animal Reproduction and Food Research, Department of Biological Function of Food, Polish Academy of Sciences, 10-748 Olsztyn, PolandDepartment of Pharmacology and Toxicology, Faculty of Medicine, University of Warmia and Mazury, 10-082 Olsztyn, PolandInstitute of Animal Reproduction and Food Research, Department of Biological Function of Food, Polish Academy of Sciences, 10-748 Olsztyn, PolandWe hypothesised that the dietary addition of the bioactive antimicrobial protein lactoferrin (LF) and peptides melittin (MT) or cecropin A (CR) at a dosage of 100 mg/kg to the diet of Wistar rats would result in strong modulatory effects on faecal microbial enzymatic activity, short-chain fatty acid and ammonia concentrations. To date, the changes in bacterial extracellular and intracellular enzymatic activities upon addition of dietary AMPs have not yet been studied. This experiment lasted 15 days; during the first 5 day period, the rats were fed the control diet (S) and diets supplemented with LF, MT or CR. On days 6–15, all rats were fed the control S diet. The faecal fermentation processes were substantially stopped after two days of treatment, on average, in all rats receiving LF and two AMPs. The deepest suppression effect was observed on the last day of treatment (day 5) and persisted through days 5–8. The highest decreases in faecal bacterial β-glucosidase and β-glucuronidase activities as well as in SCFA and ammonia concentrations were observed in the rats fed the CR diet. Only in the CR animals did the mechanism of suppressed microbial fermentation involve diminished enzyme release from bacterial cells to the digesta.https://www.mdpi.com/2076-2615/11/5/1203antimicrobial peptidesmelittincecropin Alactoferrinfaecesbacterial enzymes |
| spellingShingle | Jerzy Juśkiewicz Aleksandra Rawicka Bartosz Fotschki Michał Majewski Zenon Zduńczyk Influence of Supplementation of Lactoferrin, Melittin and Cecropin A to Rat Diet on Changes in Faecal Ammonia Concentrations, Short-Chain Fatty Acid Concentrations and Activities of Bacterial Enzymes Animals antimicrobial peptides melittin cecropin A lactoferrin faeces bacterial enzymes |
| title | Influence of Supplementation of Lactoferrin, Melittin and Cecropin A to Rat Diet on Changes in Faecal Ammonia Concentrations, Short-Chain Fatty Acid Concentrations and Activities of Bacterial Enzymes |
| title_full | Influence of Supplementation of Lactoferrin, Melittin and Cecropin A to Rat Diet on Changes in Faecal Ammonia Concentrations, Short-Chain Fatty Acid Concentrations and Activities of Bacterial Enzymes |
| title_fullStr | Influence of Supplementation of Lactoferrin, Melittin and Cecropin A to Rat Diet on Changes in Faecal Ammonia Concentrations, Short-Chain Fatty Acid Concentrations and Activities of Bacterial Enzymes |
| title_full_unstemmed | Influence of Supplementation of Lactoferrin, Melittin and Cecropin A to Rat Diet on Changes in Faecal Ammonia Concentrations, Short-Chain Fatty Acid Concentrations and Activities of Bacterial Enzymes |
| title_short | Influence of Supplementation of Lactoferrin, Melittin and Cecropin A to Rat Diet on Changes in Faecal Ammonia Concentrations, Short-Chain Fatty Acid Concentrations and Activities of Bacterial Enzymes |
| title_sort | influence of supplementation of lactoferrin melittin and cecropin a to rat diet on changes in faecal ammonia concentrations short chain fatty acid concentrations and activities of bacterial enzymes |
| topic | antimicrobial peptides melittin cecropin A lactoferrin faeces bacterial enzymes |
| url | https://www.mdpi.com/2076-2615/11/5/1203 |
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