Streptomyces turgidiscabies Possesses a Functional Cytokinin Biosynthetic Pathway and Produces Leafy Galls
Streptomyces turgidiscabies, a cause of potato scab, possesses a mobilizable pathogenicity island containing multiple virulence genes and a cytokinin biosynthetic pathway. These biosynthetic genes are homologous and collinear with the fas operon in Rhodococcus fascians. Reverse-transcriptase polymer...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2007-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-7-0751 |
| _version_ | 1819082090477518848 |
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| author | Madhumita V. Joshi Rosemary Loria |
| author_facet | Madhumita V. Joshi Rosemary Loria |
| author_sort | Madhumita V. Joshi |
| collection | DOAJ |
| description | Streptomyces turgidiscabies, a cause of potato scab, possesses a mobilizable pathogenicity island containing multiple virulence genes and a cytokinin biosynthetic pathway. These biosynthetic genes are homologous and collinear with the fas operon in Rhodococcus fascians. Reverse-transcriptase polymerase chain reaction of S. turgidiscabies demonstrated that all six genes were transcribed in oat bran broth with and without glucose, though transcription was partially repressed by glucose. The supernatant of S. turgidiscabies cultures had cytokinin activity in callus initiation and differentiation assays. Arabidopsis and tobacco plants inoculated with a thaxtomin-deficient mutant (Δnos) produced leafy galls, indistinguishable from those produced by R. fascians. Deletion of the ipt gene in the pathway eliminated gall phenotype. Other symptoms on tobacco included production of hairy roots and de novo meristems. |
| first_indexed | 2024-12-21T20:11:09Z |
| format | Article |
| id | doaj.art-7704dc0f51b240f4acfcb7d06b5da6de |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T20:11:09Z |
| publishDate | 2007-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-7704dc0f51b240f4acfcb7d06b5da6de2022-12-21T18:51:44ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-07-0120775175810.1094/MPMI-20-7-0751Streptomyces turgidiscabies Possesses a Functional Cytokinin Biosynthetic Pathway and Produces Leafy GallsMadhumita V. JoshiRosemary LoriaStreptomyces turgidiscabies, a cause of potato scab, possesses a mobilizable pathogenicity island containing multiple virulence genes and a cytokinin biosynthetic pathway. These biosynthetic genes are homologous and collinear with the fas operon in Rhodococcus fascians. Reverse-transcriptase polymerase chain reaction of S. turgidiscabies demonstrated that all six genes were transcribed in oat bran broth with and without glucose, though transcription was partially repressed by glucose. The supernatant of S. turgidiscabies cultures had cytokinin activity in callus initiation and differentiation assays. Arabidopsis and tobacco plants inoculated with a thaxtomin-deficient mutant (Δnos) produced leafy galls, indistinguishable from those produced by R. fascians. Deletion of the ipt gene in the pathway eliminated gall phenotype. Other symptoms on tobacco included production of hairy roots and de novo meristems.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-7-0751cytokinin biosynthesis |
| spellingShingle | Madhumita V. Joshi Rosemary Loria Streptomyces turgidiscabies Possesses a Functional Cytokinin Biosynthetic Pathway and Produces Leafy Galls Molecular Plant-Microbe Interactions cytokinin biosynthesis |
| title | Streptomyces turgidiscabies Possesses a Functional Cytokinin Biosynthetic Pathway and Produces Leafy Galls |
| title_full | Streptomyces turgidiscabies Possesses a Functional Cytokinin Biosynthetic Pathway and Produces Leafy Galls |
| title_fullStr | Streptomyces turgidiscabies Possesses a Functional Cytokinin Biosynthetic Pathway and Produces Leafy Galls |
| title_full_unstemmed | Streptomyces turgidiscabies Possesses a Functional Cytokinin Biosynthetic Pathway and Produces Leafy Galls |
| title_short | Streptomyces turgidiscabies Possesses a Functional Cytokinin Biosynthetic Pathway and Produces Leafy Galls |
| title_sort | streptomyces turgidiscabies possesses a functional cytokinin biosynthetic pathway and produces leafy galls |
| topic | cytokinin biosynthesis |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-7-0751 |
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