Disinfection, germination and micropropagation in vitro of Apuleia leiocarpa (Vogel) Macbride
The present study aimed to establish the protocols for disinfestations, germination and in vitro micropropagation of grápia (Apuleia leiocarpa (Vogel) Macbride). To disinfestations and break seed dormancy, were tested three treatments, and inoculated into test tubes containing growing medium. As for...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Rondonópolis
2016-07-01
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| Series: | Scientific Electronic Archives |
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| Online Access: | https://sea.ufr.edu.br/index.php?journal=SEA&page=article&op=view&path=215 |
| _version_ | 1828794579776700416 |
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| author | D. Fabris T. Gerber L. M. Sartoretto |
| author_facet | D. Fabris T. Gerber L. M. Sartoretto |
| author_sort | D. Fabris |
| collection | DOAJ |
| description | The present study aimed to establish the protocols for disinfestations, germination and in vitro micropropagation of grápia (Apuleia leiocarpa (Vogel) Macbride). To disinfestations and break seed dormancy, were tested three treatments, and inoculated into test tubes containing growing medium. As for variable germination were tested four medium of nutritious, MS, MS½, WPM, WPM ½. And, to introduce organogenesis, the growing medium used was WPM, supplemented with BAP, at concentrations of 0,0; 0,4; 0,8 ml/L, combined with ANA in the concentration of 0,05 ml/L. The explants used in this study were hypocotyls and cotyledons obtained from grapia seedling germinated in vitro, 30 days after inoculation in growing medium. The organogenesis induction was performed in WPM growing culture. The evaluated parameters in this study were the disinfestation and break dormancy, germination and organogenesis induction. The data were subjected to variance analysis and the by Tukey test, at 95% of probability. Treatment III showed to be more efficient for both aseptic and to break seed dormancy, providing 92% of germination and 2% of contamination. As for the in vitro germination, the WPM and WPM½ growing cultures were the most effective, favoring 100% of germination. For various formations of calluses and sprouts on hypocotyls explants, there was no significant difference between the tested BAP concentrations. However, it was observed in cotyledon explants that the best results occurred at concentrations of 0,8 ml and 0,4 ml of BAP and 0,05 ml of ANA. |
| first_indexed | 2024-12-12T03:47:54Z |
| format | Article |
| id | doaj.art-7707283e0c9b484594e7b05831cf22e9 |
| institution | Directory Open Access Journal |
| issn | 2316-9281 2316-9281 |
| language | English |
| last_indexed | 2024-12-12T03:47:54Z |
| publishDate | 2016-07-01 |
| publisher | Universidade Federal de Rondonópolis |
| record_format | Article |
| series | Scientific Electronic Archives |
| spelling | doaj.art-7707283e0c9b484594e7b05831cf22e92022-12-22T00:39:28ZengUniversidade Federal de RondonópolisScientific Electronic Archives2316-92812316-92812016-07-0193172610.36560/932016215159Disinfection, germination and micropropagation in vitro of Apuleia leiocarpa (Vogel) MacbrideD. Fabris0T. Gerber1L. M. Sartoretto2Universidade do Oeste de Santa Catarina - UNOESCFaculdade Concórdia - FACCFaculdade Concórdia - FACC.The present study aimed to establish the protocols for disinfestations, germination and in vitro micropropagation of grápia (Apuleia leiocarpa (Vogel) Macbride). To disinfestations and break seed dormancy, were tested three treatments, and inoculated into test tubes containing growing medium. As for variable germination were tested four medium of nutritious, MS, MS½, WPM, WPM ½. And, to introduce organogenesis, the growing medium used was WPM, supplemented with BAP, at concentrations of 0,0; 0,4; 0,8 ml/L, combined with ANA in the concentration of 0,05 ml/L. The explants used in this study were hypocotyls and cotyledons obtained from grapia seedling germinated in vitro, 30 days after inoculation in growing medium. The organogenesis induction was performed in WPM growing culture. The evaluated parameters in this study were the disinfestation and break dormancy, germination and organogenesis induction. The data were subjected to variance analysis and the by Tukey test, at 95% of probability. Treatment III showed to be more efficient for both aseptic and to break seed dormancy, providing 92% of germination and 2% of contamination. As for the in vitro germination, the WPM and WPM½ growing cultures were the most effective, favoring 100% of germination. For various formations of calluses and sprouts on hypocotyls explants, there was no significant difference between the tested BAP concentrations. However, it was observed in cotyledon explants that the best results occurred at concentrations of 0,8 ml and 0,4 ml of BAP and 0,05 ml of ANA.https://sea.ufr.edu.br/index.php?journal=SEA&page=article&op=view&path=215germination, break dormancy, organogenesis |
| spellingShingle | D. Fabris T. Gerber L. M. Sartoretto Disinfection, germination and micropropagation in vitro of Apuleia leiocarpa (Vogel) Macbride Scientific Electronic Archives germination, break dormancy, organogenesis |
| title | Disinfection, germination and micropropagation in vitro of Apuleia leiocarpa (Vogel) Macbride |
| title_full | Disinfection, germination and micropropagation in vitro of Apuleia leiocarpa (Vogel) Macbride |
| title_fullStr | Disinfection, germination and micropropagation in vitro of Apuleia leiocarpa (Vogel) Macbride |
| title_full_unstemmed | Disinfection, germination and micropropagation in vitro of Apuleia leiocarpa (Vogel) Macbride |
| title_short | Disinfection, germination and micropropagation in vitro of Apuleia leiocarpa (Vogel) Macbride |
| title_sort | disinfection germination and micropropagation in vitro of apuleia leiocarpa vogel macbride |
| topic | germination, break dormancy, organogenesis |
| url | https://sea.ufr.edu.br/index.php?journal=SEA&page=article&op=view&path=215 |
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