Polymorphism of the <i>MTNR1A</i> Melatonin Receptor Gene in Goat Breeds of Northern Italy
Melatonin receptor MT1, encoded by the <i>MTNR1A</i> gene, is the main one involved in the seasonal regulation of reproductive activity. A correlation between this gene polymorphism and reproductive performance has been demonstrated in sheep. To date, no data about <i>MTNR1A</i&...
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MDPI AG
2023-12-01
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| author | Stella Agradi Sebastiano Luridiana Maria Consuelo Mura Giovanni Cosso Daniele Vigo Laura Menchetti Gabriele Brecchia Vincenzo Carcangiu Giulio Curone |
| author_facet | Stella Agradi Sebastiano Luridiana Maria Consuelo Mura Giovanni Cosso Daniele Vigo Laura Menchetti Gabriele Brecchia Vincenzo Carcangiu Giulio Curone |
| author_sort | Stella Agradi |
| collection | DOAJ |
| description | Melatonin receptor MT1, encoded by the <i>MTNR1A</i> gene, is the main one involved in the seasonal regulation of reproductive activity. A correlation between this gene polymorphism and reproductive performance has been demonstrated in sheep. To date, no data about <i>MTNR1A</i> gene polymorphism are available regarding Italian goat breeds other than the Sarda goat. This study aimed to detect any PCR-RFLP polymorphic sites of <i>MTNR1A</i> using <i>MnlI</i> and <i>RsaI</i> enzymes in Northern Italian goat breeds, which are characterized by a pronounced reproductive seasonality. One-hundred-eight adult female goats belonging to four different breeds were included in the study (i.e., Frisa Valtellinese, <i>n</i> = 29; Orobica, <i>n</i> = 23; Lariana, <i>n</i> = 29; Camosciata delle Alpi, <i>n</i> = 27). Blood was sampled from each goat. Genomic DNA was extracted from each sample and the main part of exon II of <i>MTNR1A</i> gene was amplified by PCR and digested with <i>MnlI</i> and <i>RsaI</i> enzymes. Unexpectedly, none of the fragments were found to be polymorphic. The absence of polymorphism may be linked to the macro group of goat breeds that evolved during human migrations. Breeds of the Alpine–European strain would appear to show no polymorphism, as confirmed by our study, whereas breeds belonging to the Mediterranean–African or Asian–Middle Eastern strains do. |
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| issn | 2076-2615 |
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| last_indexed | 2024-03-08T21:03:36Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-7709918ee3534b5794c4868748ba3bdc2023-12-22T13:47:38ZengMDPI AGAnimals2076-26152023-12-011324385510.3390/ani13243855Polymorphism of the <i>MTNR1A</i> Melatonin Receptor Gene in Goat Breeds of Northern ItalyStella Agradi0Sebastiano Luridiana1Maria Consuelo Mura2Giovanni Cosso3Daniele Vigo4Laura Menchetti5Gabriele Brecchia6Vincenzo Carcangiu7Giulio Curone8Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, ItalyDepartment of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, Via Circonvallazione 93/95, 62024 Matelica, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyDepartment of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, Via dell’Università 6, 26900 Lodi, ItalyMelatonin receptor MT1, encoded by the <i>MTNR1A</i> gene, is the main one involved in the seasonal regulation of reproductive activity. A correlation between this gene polymorphism and reproductive performance has been demonstrated in sheep. To date, no data about <i>MTNR1A</i> gene polymorphism are available regarding Italian goat breeds other than the Sarda goat. This study aimed to detect any PCR-RFLP polymorphic sites of <i>MTNR1A</i> using <i>MnlI</i> and <i>RsaI</i> enzymes in Northern Italian goat breeds, which are characterized by a pronounced reproductive seasonality. One-hundred-eight adult female goats belonging to four different breeds were included in the study (i.e., Frisa Valtellinese, <i>n</i> = 29; Orobica, <i>n</i> = 23; Lariana, <i>n</i> = 29; Camosciata delle Alpi, <i>n</i> = 27). Blood was sampled from each goat. Genomic DNA was extracted from each sample and the main part of exon II of <i>MTNR1A</i> gene was amplified by PCR and digested with <i>MnlI</i> and <i>RsaI</i> enzymes. Unexpectedly, none of the fragments were found to be polymorphic. The absence of polymorphism may be linked to the macro group of goat breeds that evolved during human migrations. Breeds of the Alpine–European strain would appear to show no polymorphism, as confirmed by our study, whereas breeds belonging to the Mediterranean–African or Asian–Middle Eastern strains do.https://www.mdpi.com/2076-2615/13/24/3855local breedmelatonin receptor geneseasonal reproductionsmall ruminants |
| spellingShingle | Stella Agradi Sebastiano Luridiana Maria Consuelo Mura Giovanni Cosso Daniele Vigo Laura Menchetti Gabriele Brecchia Vincenzo Carcangiu Giulio Curone Polymorphism of the <i>MTNR1A</i> Melatonin Receptor Gene in Goat Breeds of Northern Italy Animals local breed melatonin receptor gene seasonal reproduction small ruminants |
| title | Polymorphism of the <i>MTNR1A</i> Melatonin Receptor Gene in Goat Breeds of Northern Italy |
| title_full | Polymorphism of the <i>MTNR1A</i> Melatonin Receptor Gene in Goat Breeds of Northern Italy |
| title_fullStr | Polymorphism of the <i>MTNR1A</i> Melatonin Receptor Gene in Goat Breeds of Northern Italy |
| title_full_unstemmed | Polymorphism of the <i>MTNR1A</i> Melatonin Receptor Gene in Goat Breeds of Northern Italy |
| title_short | Polymorphism of the <i>MTNR1A</i> Melatonin Receptor Gene in Goat Breeds of Northern Italy |
| title_sort | polymorphism of the i mtnr1a i melatonin receptor gene in goat breeds of northern italy |
| topic | local breed melatonin receptor gene seasonal reproduction small ruminants |
| url | https://www.mdpi.com/2076-2615/13/24/3855 |
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