Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis.
Coenzyme Q10 (CoQ10) is a powerful antioxidant with a myriad of applications in healthcare and cosmetic industries. The most effective route of CoQ10 production is microbial biosynthesis. In this study, four CoQ10 biosynthesizing purple photosynthetic bacteria: Rhodobacter blasticus, Rhodovulum adri...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X2200073X |
| _version_ | 1811312957147054080 |
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| author | Drishya M. George Ramya Ramadoss Hamish R. Mackey Annette S. Vincent |
| author_facet | Drishya M. George Ramya Ramadoss Hamish R. Mackey Annette S. Vincent |
| author_sort | Drishya M. George |
| collection | DOAJ |
| description | Coenzyme Q10 (CoQ10) is a powerful antioxidant with a myriad of applications in healthcare and cosmetic industries. The most effective route of CoQ10 production is microbial biosynthesis. In this study, four CoQ10 biosynthesizing purple photosynthetic bacteria: Rhodobacter blasticus, Rhodovulum adriaticum, Afifella pfennigii and Rhodovulum marinum, were identified using 16S rRNA sequencing of enriched microbial mat samples obtained from Purple Island mangroves (Qatar). The membrane bound enzyme 4-hydroxybenzoate octaprenyltransferase (UbiA) is pivotal for bacterial biosynthesis of CoQ10. The identified bacteria could be inducted as efficient industrial bio-synthesizers of CoQ10 by engineering their UbiA enzymes. Therefore, the mutation sites and substitution residues for potential functional enhancement were determined by comparative computational study. Two mutation sites were identified within the two conserved Asp-rich motifs, and the effect of proposed mutations in substrate binding affinity of the UbiA enzymes was assessed using multiple ligand simultaneous docking (MLSD) studies, as a groundwork for experimental studies. |
| first_indexed | 2024-04-13T10:45:46Z |
| format | Article |
| id | doaj.art-770b3c33cd124479bf452ee3500b43e0 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-13T10:45:46Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-770b3c33cd124479bf452ee3500b43e02022-12-22T02:49:48ZengElsevierBiotechnology Reports2215-017X2022-12-0136e00775Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis.Drishya M. George0Ramya Ramadoss1Hamish R. Mackey2Annette S. Vincent3College of Health and Life Sciences, Hamad bin Khalifa University, Qatar Foundation, Doha, QatarBiological Sciences, Carnegie Mellon University Qatar, Doha, QatarCollege of Health and Life Sciences, Hamad bin Khalifa University, Qatar Foundation, Doha, Qatar; Division of Sustainable Development, College of Science and Engineering, Hamad bin Khalifa University, Qatar Foundation, Doha, QatarBiological Sciences, Carnegie Mellon University Qatar, Doha, Qatar; Corresponding author.Coenzyme Q10 (CoQ10) is a powerful antioxidant with a myriad of applications in healthcare and cosmetic industries. The most effective route of CoQ10 production is microbial biosynthesis. In this study, four CoQ10 biosynthesizing purple photosynthetic bacteria: Rhodobacter blasticus, Rhodovulum adriaticum, Afifella pfennigii and Rhodovulum marinum, were identified using 16S rRNA sequencing of enriched microbial mat samples obtained from Purple Island mangroves (Qatar). The membrane bound enzyme 4-hydroxybenzoate octaprenyltransferase (UbiA) is pivotal for bacterial biosynthesis of CoQ10. The identified bacteria could be inducted as efficient industrial bio-synthesizers of CoQ10 by engineering their UbiA enzymes. Therefore, the mutation sites and substitution residues for potential functional enhancement were determined by comparative computational study. Two mutation sites were identified within the two conserved Asp-rich motifs, and the effect of proposed mutations in substrate binding affinity of the UbiA enzymes was assessed using multiple ligand simultaneous docking (MLSD) studies, as a groundwork for experimental studies.http://www.sciencedirect.com/science/article/pii/S2215017X2200073XAnoxygenic phototrophic bacteria (APB)Resource recoveryPurple photosynthetic bacteria (PPB)Coenzyme Q10 (CoQ10)Protein modelingMultiple ligand simultaneous docking (MLSD) |
| spellingShingle | Drishya M. George Ramya Ramadoss Hamish R. Mackey Annette S. Vincent Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis. Biotechnology Reports Anoxygenic phototrophic bacteria (APB) Resource recovery Purple photosynthetic bacteria (PPB) Coenzyme Q10 (CoQ10) Protein modeling Multiple ligand simultaneous docking (MLSD) |
| title | Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis. |
| title_full | Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis. |
| title_fullStr | Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis. |
| title_full_unstemmed | Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis. |
| title_short | Comparative computational study to augment UbiA prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in Qatar for coenzyme Q10 biosynthesis. |
| title_sort | comparative computational study to augment ubia prenyltransferases inherent in purple photosynthetic bacteria cultured from mangrove microbial mats in qatar for coenzyme q10 biosynthesis |
| topic | Anoxygenic phototrophic bacteria (APB) Resource recovery Purple photosynthetic bacteria (PPB) Coenzyme Q10 (CoQ10) Protein modeling Multiple ligand simultaneous docking (MLSD) |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X2200073X |
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