Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins
<p>Abstract</p> <p>Background</p> <p>The human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. To better understand the influence of virus on host cells, we performed the differential proteome research of peripheral blood monon...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-03-01
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| Series: | Proteome Science |
| Online Access: | http://www.proteomesci.com/content/8/1/12 |
| _version_ | 1818272184143970304 |
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| author | Yin Lin Ma Fang Qi Tangkai Peng Xia Sun Jianjun Zhang Xiaojun Jia Xiaofang Zhang Lijun Yao Yamin Qiu Chao Lu Hongzhou |
| author_facet | Yin Lin Ma Fang Qi Tangkai Peng Xia Sun Jianjun Zhang Xiaojun Jia Xiaofang Zhang Lijun Yao Yamin Qiu Chao Lu Hongzhou |
| author_sort | Yin Lin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. To better understand the influence of virus on host cells, we performed the differential proteome research of peripheral blood mononuclear cells (PBMCs) from HIV-positive patients and healthy controls.</p> <p>Results</p> <p>26 protein spots with more than 1.5-fold difference were detected in two dimensional electrophoresis (2DE) gels. 12 unique up-regulated and one down-regulated proteins were identified in HIV-positive patients compared with healthy donors. The mRNA expression of 10 genes was analyzed by real time RT-PCR. It shows that the mRNA expression of talin-1, vinculin and coronin-1C were up-regulated in HIV positive patients and consistent with protein expression. Western blotting analysis confirmed the induction of fragments of vinculin, talin-1 and filamin-A in pooled and most part of individual HIV-positive clinical samples. Bioinformatic analysis showed that a wide host protein network was disrupted in HIV-positive patients.</p> <p>Conclusions</p> <p>Together, this work provided useful information to facilitate further investigation of the underlying mechanism of HIV and host cell protein interactions, and discovered novel potential biomarkers such as fragment of vinculin, filamin-A and talin-1 for anti-HIV research.</p> |
| first_indexed | 2024-12-12T21:38:02Z |
| format | Article |
| id | doaj.art-770cb0cb9d754fe8b0c9458adc893fd0 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-12T21:38:02Z |
| publishDate | 2010-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-770cb0cb9d754fe8b0c9458adc893fd02022-12-22T00:11:08ZengBMCProteome Science1477-59562010-03-01811210.1186/1477-5956-8-12Proteomic analysis of PBMCs: characterization of potential HIV-associated proteinsYin LinMa FangQi TangkaiPeng XiaSun JianjunZhang XiaojunJia XiaofangZhang LijunYao YaminQiu ChaoLu Hongzhou<p>Abstract</p> <p>Background</p> <p>The human immunodeficiency virus type 1 (HIV-1) pandemic has continued unabated for nearly 30 years. To better understand the influence of virus on host cells, we performed the differential proteome research of peripheral blood mononuclear cells (PBMCs) from HIV-positive patients and healthy controls.</p> <p>Results</p> <p>26 protein spots with more than 1.5-fold difference were detected in two dimensional electrophoresis (2DE) gels. 12 unique up-regulated and one down-regulated proteins were identified in HIV-positive patients compared with healthy donors. The mRNA expression of 10 genes was analyzed by real time RT-PCR. It shows that the mRNA expression of talin-1, vinculin and coronin-1C were up-regulated in HIV positive patients and consistent with protein expression. Western blotting analysis confirmed the induction of fragments of vinculin, talin-1 and filamin-A in pooled and most part of individual HIV-positive clinical samples. Bioinformatic analysis showed that a wide host protein network was disrupted in HIV-positive patients.</p> <p>Conclusions</p> <p>Together, this work provided useful information to facilitate further investigation of the underlying mechanism of HIV and host cell protein interactions, and discovered novel potential biomarkers such as fragment of vinculin, filamin-A and talin-1 for anti-HIV research.</p>http://www.proteomesci.com/content/8/1/12 |
| spellingShingle | Yin Lin Ma Fang Qi Tangkai Peng Xia Sun Jianjun Zhang Xiaojun Jia Xiaofang Zhang Lijun Yao Yamin Qiu Chao Lu Hongzhou Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins Proteome Science |
| title | Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins |
| title_full | Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins |
| title_fullStr | Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins |
| title_full_unstemmed | Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins |
| title_short | Proteomic analysis of PBMCs: characterization of potential HIV-associated proteins |
| title_sort | proteomic analysis of pbmcs characterization of potential hiv associated proteins |
| url | http://www.proteomesci.com/content/8/1/12 |
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