Fabrication and evaluation of herbal beads to slow cell ageing
Several therapies and cosmetics are available commercially to prevent or delay cell ageing, which manifests as premature cell death and skin dullness. Use of herbal products such as Aloe vera, curcumin, vitamin C-enriched natural antioxidant, and anti-inflammatory biomolecules are potential ways to...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1025405/full |
| _version_ | 1797655989538783232 |
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| author | Archna Dhasmana Sumira Malik Amit Kumar Sharma Anuj Ranjan Abhishek Chauhan Steve Harakeh Rajaa M. Al-Raddadi Majed N. Almashjary Majed N. Almashjary Majed N. Almashjary Waleed Mohammed S. Bawazir Waleed Mohammed S. Bawazir Shafiul Haque |
| author_facet | Archna Dhasmana Sumira Malik Amit Kumar Sharma Anuj Ranjan Abhishek Chauhan Steve Harakeh Rajaa M. Al-Raddadi Majed N. Almashjary Majed N. Almashjary Majed N. Almashjary Waleed Mohammed S. Bawazir Waleed Mohammed S. Bawazir Shafiul Haque |
| author_sort | Archna Dhasmana |
| collection | DOAJ |
| description | Several therapies and cosmetics are available commercially to prevent or delay cell ageing, which manifests as premature cell death and skin dullness. Use of herbal products such as Aloe vera, curcumin, vitamin C-enriched natural antioxidant, and anti-inflammatory biomolecules are potential ways to prevent or delay ageing. Eggshell membrane (ESM) is also a rich source of collagen; glycosaminoglycans (GAGs) also play an essential role in healing and preventing ageing. It is important to use an extended therapeutic process to prolong the effectiveness of these products, despite the fact that they all have significant anti-ageing properties and the ability to regenerate healthy cells. Encapsulated herbal components are therefore designed to overcome the challenge of ensuring continued treatment over time to prolong the effects of a bioactive component after in situ administration. To study their synergistic effects on a cellular level, alginate, Aloe vera, and orange peel extract were encapsulated in bio-polymeric foaming beads and modified with eggshell membrane protein (ESMP) at various concentrations (1 gm, 2 gm, and 5 gm): (A-Av-OP, A-Av-OP-ESMP1, ESMP2, and ESMP3). Analysis of the structural and functional properties of foaming beads showed interconnected 3D porous structure, a surface-functionalized group for entrapment of ESMP, and a significant reduction in pore size (51–35 m) and porosity (80%–60%). By performing DPPH assays, HRBC stabilization assays, and antibacterial tests, the beads were assessed as a natural anti-ageing product with sustained release of molecules effective against inflammatory response, oxidative stress, and microbial contamination. MTT assays were conducted using in vitro cell cultures to demonstrate cytocompatibility (in mouse 3T3 fibroblast cells) and cytotoxicity (in human carcinoma HeLa cells). Our study demonstrates that bio-polymeric ESMP beads up to 2 g (A-Av-OP-ESMP2) are practical and feasible natural remedies for suspending defective cell pathways, preventing cell ageing, and promoting healthy cell growth, resulting in a viable and practical natural remedy or therapeutic system. |
| first_indexed | 2024-03-11T17:22:40Z |
| format | Article |
| id | doaj.art-770e4c88d009491c89b46911d6e18aa8 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-11T17:22:40Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-770e4c88d009491c89b46911d6e18aa82023-10-19T09:59:42ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-12-011010.3389/fbioe.2022.10254051025405Fabrication and evaluation of herbal beads to slow cell ageingArchna Dhasmana0Sumira Malik1Amit Kumar Sharma2Anuj Ranjan3Abhishek Chauhan4Steve Harakeh5Rajaa M. Al-Raddadi6Majed N. Almashjary7Majed N. Almashjary8Majed N. Almashjary9Waleed Mohammed S. Bawazir10Waleed Mohammed S. Bawazir11Shafiul Haque12Himalayan School of Biosciences, Swami Rama Himalayan University, Jolly Grant, Dehradun, Uttarakhand, IndiaAmity Institute of Biotechnology, Amity University Jharkhand, Ranchi, Jharkhand, IndiaDepartment of Biotechnology, Dr KNMIPER, Modinagar, Uttar Pradesh, IndiaAcademy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, RussiaAmity Institute of Environmental Toxicology, Safety and Management, Amity University, Noida, IndiaKing Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Community Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi ArabiaHematology Research Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia0Animal House Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi ArabiaHematology Research Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia1Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia2Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan, Saudi ArabiaSeveral therapies and cosmetics are available commercially to prevent or delay cell ageing, which manifests as premature cell death and skin dullness. Use of herbal products such as Aloe vera, curcumin, vitamin C-enriched natural antioxidant, and anti-inflammatory biomolecules are potential ways to prevent or delay ageing. Eggshell membrane (ESM) is also a rich source of collagen; glycosaminoglycans (GAGs) also play an essential role in healing and preventing ageing. It is important to use an extended therapeutic process to prolong the effectiveness of these products, despite the fact that they all have significant anti-ageing properties and the ability to regenerate healthy cells. Encapsulated herbal components are therefore designed to overcome the challenge of ensuring continued treatment over time to prolong the effects of a bioactive component after in situ administration. To study their synergistic effects on a cellular level, alginate, Aloe vera, and orange peel extract were encapsulated in bio-polymeric foaming beads and modified with eggshell membrane protein (ESMP) at various concentrations (1 gm, 2 gm, and 5 gm): (A-Av-OP, A-Av-OP-ESMP1, ESMP2, and ESMP3). Analysis of the structural and functional properties of foaming beads showed interconnected 3D porous structure, a surface-functionalized group for entrapment of ESMP, and a significant reduction in pore size (51–35 m) and porosity (80%–60%). By performing DPPH assays, HRBC stabilization assays, and antibacterial tests, the beads were assessed as a natural anti-ageing product with sustained release of molecules effective against inflammatory response, oxidative stress, and microbial contamination. MTT assays were conducted using in vitro cell cultures to demonstrate cytocompatibility (in mouse 3T3 fibroblast cells) and cytotoxicity (in human carcinoma HeLa cells). Our study demonstrates that bio-polymeric ESMP beads up to 2 g (A-Av-OP-ESMP2) are practical and feasible natural remedies for suspending defective cell pathways, preventing cell ageing, and promoting healthy cell growth, resulting in a viable and practical natural remedy or therapeutic system.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1025405/fullherbalquercetindruggraftbiocompatibilityherbal extracts |
| spellingShingle | Archna Dhasmana Sumira Malik Amit Kumar Sharma Anuj Ranjan Abhishek Chauhan Steve Harakeh Rajaa M. Al-Raddadi Majed N. Almashjary Majed N. Almashjary Majed N. Almashjary Waleed Mohammed S. Bawazir Waleed Mohammed S. Bawazir Shafiul Haque Fabrication and evaluation of herbal beads to slow cell ageing Frontiers in Bioengineering and Biotechnology herbal quercetin drug graft biocompatibility herbal extracts |
| title | Fabrication and evaluation of herbal beads to slow cell ageing |
| title_full | Fabrication and evaluation of herbal beads to slow cell ageing |
| title_fullStr | Fabrication and evaluation of herbal beads to slow cell ageing |
| title_full_unstemmed | Fabrication and evaluation of herbal beads to slow cell ageing |
| title_short | Fabrication and evaluation of herbal beads to slow cell ageing |
| title_sort | fabrication and evaluation of herbal beads to slow cell ageing |
| topic | herbal quercetin drug graft biocompatibility herbal extracts |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1025405/full |
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