Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis
Multiple strains of Bacillus subtilis were demonstrated to stimulate plant defense responses, and cyclic lipopeptides may be involved in the elicitation of this induced systemic resistance phenomenon. Here, we further investigated molecular events underlying the interaction between such lipopeptides...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2009-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-4-0456 |
| _version_ | 1811277626733494272 |
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| author | E. Jourdan G. Henry F. Duby J. Dommes J. P. Barthélemy P. Thonart M. Ongena |
| author_facet | E. Jourdan G. Henry F. Duby J. Dommes J. P. Barthélemy P. Thonart M. Ongena |
| author_sort | E. Jourdan |
| collection | DOAJ |
| description | Multiple strains of Bacillus subtilis were demonstrated to stimulate plant defense responses, and cyclic lipopeptides may be involved in the elicitation of this induced systemic resistance phenomenon. Here, we further investigated molecular events underlying the interaction between such lipopeptides and plant cells. Addition of surfactin but not fengycin or iturin in the micromolar range to tobacco cell suspensions induced defense-related early events such as extracellular medium alkalinization coupled with ion fluxes and reactive oxygen species production. Surfactin also stimulated the defense enzymes phenylalanine ammonia lyase and lipoxygenase and modified the pattern of phenolics produced by the elicited cells. The occurrence of these surfactin-elicited early events is closely related to Ca2+ influx and dynamic changes in protein phosphorylation but is not associated with any marked phytotoxicity or adverse effect on the integrity and growth potential of the treated tobacco cells. Reduced activity of some homologues also indicates that surfactin perception is dictated by structural clues in both the acyl moiety and cyclic peptide part. Our results suggest that these molecules could interact without irreversible pore formation but in a way sufficient to induce disturbance or transient channeling in the plasma membrane that can, in turn, activate a biochemical cascade of molecular events leading to defensive responses. The present study sheds new light not only on defense-related events induced following recognition of amphiphilic lipopeptides from Bacillus spp. but also more globally on the way elicitors from beneficial bacteria can be perceived by host plant cells. |
| first_indexed | 2024-04-13T00:20:13Z |
| format | Article |
| id | doaj.art-77130305f0554dedbebd1a993d3a63b7 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:20:13Z |
| publishDate | 2009-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-77130305f0554dedbebd1a993d3a63b72022-12-22T03:10:48ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062009-04-0122445646810.1094/MPMI-22-4-0456Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilisE. JourdanG. HenryF. DubyJ. DommesJ. P. BarthélemyP. ThonartM. OngenaMultiple strains of Bacillus subtilis were demonstrated to stimulate plant defense responses, and cyclic lipopeptides may be involved in the elicitation of this induced systemic resistance phenomenon. Here, we further investigated molecular events underlying the interaction between such lipopeptides and plant cells. Addition of surfactin but not fengycin or iturin in the micromolar range to tobacco cell suspensions induced defense-related early events such as extracellular medium alkalinization coupled with ion fluxes and reactive oxygen species production. Surfactin also stimulated the defense enzymes phenylalanine ammonia lyase and lipoxygenase and modified the pattern of phenolics produced by the elicited cells. The occurrence of these surfactin-elicited early events is closely related to Ca2+ influx and dynamic changes in protein phosphorylation but is not associated with any marked phytotoxicity or adverse effect on the integrity and growth potential of the treated tobacco cells. Reduced activity of some homologues also indicates that surfactin perception is dictated by structural clues in both the acyl moiety and cyclic peptide part. Our results suggest that these molecules could interact without irreversible pore formation but in a way sufficient to induce disturbance or transient channeling in the plasma membrane that can, in turn, activate a biochemical cascade of molecular events leading to defensive responses. The present study sheds new light not only on defense-related events induced following recognition of amphiphilic lipopeptides from Bacillus spp. but also more globally on the way elicitors from beneficial bacteria can be perceived by host plant cells.https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-4-0456 |
| spellingShingle | E. Jourdan G. Henry F. Duby J. Dommes J. P. Barthélemy P. Thonart M. Ongena Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis Molecular Plant-Microbe Interactions |
| title | Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis |
| title_full | Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis |
| title_fullStr | Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis |
| title_full_unstemmed | Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis |
| title_short | Insights into the Defense-Related Events Occurring in Plant Cells Following Perception of Surfactin-Type Lipopeptide from Bacillus subtilis |
| title_sort | insights into the defense related events occurring in plant cells following perception of surfactin type lipopeptide from bacillus subtilis |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-4-0456 |
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