Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst
Lipid-rich bovine serum albumin (LR-BSA) has been reported to increase the survival rate of blastocysts after freezing and thawing. However studies on the early development of in vitro fertilised (IVF) embryos are still insufficient in cattle. This study investigated the blastocyst productivity of i...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2023-12-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-202311-0004_lipid-rich-bovine-serum-albumin-is-beneficial-for-the-early-development-of-bovine-embryos-but-induces-lipid-dr.php |
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| author | Dae-Jin Kwon Kyung-Do Park Lee HakKyo Jae-Don Oh |
| author_facet | Dae-Jin Kwon Kyung-Do Park Lee HakKyo Jae-Don Oh |
| author_sort | Dae-Jin Kwon |
| collection | DOAJ |
| description | Lipid-rich bovine serum albumin (LR-BSA) has been reported to increase the survival rate of blastocysts after freezing and thawing. However studies on the early development of in vitro fertilised (IVF) embryos are still insufficient in cattle. This study investigated the blastocyst productivity of in vitro culture (IVC) medium (synthetic oviductal fluid medium with amino acids, mSOFaa) supplemented with 5% fetal bovine serum (FBS, Control) or LR-BSA (1, 2.5, 5 mg/ml dosages, respectively) and the qualitative characteristics of produced blastocysts. Cleavage rates on day 2 were similar between all groups. Significant differences were obtained in the blastocyst rate in the 2.5LR-BSA group compared with the control (23.5% vs 35.7%, P < 0.05). Hatching rates were significantly higher in all treatment groups than in control (P < 0.05). The cell number in blastocysts did not differ among groups, but the apoptotic index in the 5.0LR-BSA group was higher than the control (P < 0.05). The expression of embryo quality-related markers was affected. Octamer-binding transcription factor 4 (Oct4), placenta associated 8 (Plac8), and sex-determining region Y-box 2 (Sox2) genes expression in the 2.5LR-BSA group were significantly up-regulated compared to the control (P < 0.05). Cytoplasmic lipid contents and the lipid droplet formation-related gene, sterol regulatory element binding transcription factor 1 (Srebf1), were significantly increased in the 5.0LR-BSA group (P < 0.05). Therefore, the LR-BSA supplementation (2.5 mg/ml) to mSOFaa medium could contribute to the early development of bovine IVF embryos and the qualitative improvement of the resulting blastocysts. |
| first_indexed | 2024-03-08T09:26:59Z |
| format | Article |
| id | doaj.art-771307b6ba994a4fabc4b998ddef498b |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-03-08T09:26:59Z |
| publishDate | 2023-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-771307b6ba994a4fabc4b998ddef498b2024-01-31T07:58:40ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092023-12-01681144345010.17221/107/2023-CJAScjs-202311-0004Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocystDae-Jin Kwon0Kyung-Do Park1Lee HakKyo2Jae-Don Oh3Department of Animal Biotechnology, Jeonbuk National University, Jeonju, Republic of KoreaDepartment of Animal Biotechnology, Jeonbuk National University, Jeonju, Republic of KoreaDepartment of Animal Biotechnology, Jeonbuk National University, Jeonju, Republic of KoreaDepartment of Animal Biotechnology, Jeonbuk National University, Jeonju, Republic of KoreaLipid-rich bovine serum albumin (LR-BSA) has been reported to increase the survival rate of blastocysts after freezing and thawing. However studies on the early development of in vitro fertilised (IVF) embryos are still insufficient in cattle. This study investigated the blastocyst productivity of in vitro culture (IVC) medium (synthetic oviductal fluid medium with amino acids, mSOFaa) supplemented with 5% fetal bovine serum (FBS, Control) or LR-BSA (1, 2.5, 5 mg/ml dosages, respectively) and the qualitative characteristics of produced blastocysts. Cleavage rates on day 2 were similar between all groups. Significant differences were obtained in the blastocyst rate in the 2.5LR-BSA group compared with the control (23.5% vs 35.7%, P < 0.05). Hatching rates were significantly higher in all treatment groups than in control (P < 0.05). The cell number in blastocysts did not differ among groups, but the apoptotic index in the 5.0LR-BSA group was higher than the control (P < 0.05). The expression of embryo quality-related markers was affected. Octamer-binding transcription factor 4 (Oct4), placenta associated 8 (Plac8), and sex-determining region Y-box 2 (Sox2) genes expression in the 2.5LR-BSA group were significantly up-regulated compared to the control (P < 0.05). Cytoplasmic lipid contents and the lipid droplet formation-related gene, sterol regulatory element binding transcription factor 1 (Srebf1), were significantly increased in the 5.0LR-BSA group (P < 0.05). Therefore, the LR-BSA supplementation (2.5 mg/ml) to mSOFaa medium could contribute to the early development of bovine IVF embryos and the qualitative improvement of the resulting blastocysts.https://cjas.agriculturejournals.cz/artkey/cjs-202311-0004_lipid-rich-bovine-serum-albumin-is-beneficial-for-the-early-development-of-bovine-embryos-but-induces-lipid-dr.phpcattleapoptosisembryo qualityin vitro fertilised embryo |
| spellingShingle | Dae-Jin Kwon Kyung-Do Park Lee HakKyo Jae-Don Oh Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst Czech Journal of Animal Science cattle apoptosis embryo quality in vitro fertilised embryo |
| title | Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst |
| title_full | Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst |
| title_fullStr | Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst |
| title_full_unstemmed | Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst |
| title_short | Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst |
| title_sort | lipid rich bovine serum albumin is beneficial for the early development of bovine embryos but induces lipid droplet formation in the blastocyst |
| topic | cattle apoptosis embryo quality in vitro fertilised embryo |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-202311-0004_lipid-rich-bovine-serum-albumin-is-beneficial-for-the-early-development-of-bovine-embryos-but-induces-lipid-dr.php |
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