Recombinant banana lectin as mucosal immunostimulator
To explore the potential usage of recombinant banana lectin as an immunostimulator it was of interest to analyze the effect it exerted in vivo in mice, upon two different routes of exposure. By Western blotting analysis of the gastric and intestinal luminal content it was found that recombinant bana...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-07-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464612000515 |
| _version_ | 1831641284471685120 |
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| author | R. Dimitrijevic M. Stojanovic M. Micic Lj. Dimitrijevic M. Gavrovic-Jankulovic |
| author_facet | R. Dimitrijevic M. Stojanovic M. Micic Lj. Dimitrijevic M. Gavrovic-Jankulovic |
| author_sort | R. Dimitrijevic |
| collection | DOAJ |
| description | To explore the potential usage of recombinant banana lectin as an immunostimulator it was of interest to analyze the effect it exerted in vivo in mice, upon two different routes of exposure. By Western blotting analysis of the gastric and intestinal luminal content it was found that recombinant banana lectin is stable in vivo in the mouse digestive tract, where it specifically interacts with the mucosal surfaces which can be inhibited by the addition of glucose. Its attachment to mice jejunum has been confirmed by immunofluorescence. In addition, it was able to induce systemic immunity, evidenced by specific antibody production, by oral application, a property that might be exploited for the induction of systemic immune responses. |
| first_indexed | 2024-12-19T12:21:33Z |
| format | Article |
| id | doaj.art-771607abe6524984bfac18ad634df39d |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-19T12:21:33Z |
| publishDate | 2012-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-771607abe6524984bfac18ad634df39d2022-12-21T20:21:45ZengElsevierJournal of Functional Foods1756-46462012-07-0143636641Recombinant banana lectin as mucosal immunostimulatorR. Dimitrijevic0M. Stojanovic1M. Micic2Lj. Dimitrijevic3M. Gavrovic-Jankulovic4Innovation Center of Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, Serbia; Corresponding author.Department of Research and Development, Institute of Virology, Vaccines and Sera-Torlak, Vojvode Stepe 458, 11152 Belgrade, SerbiaInstitute for Medical Research, University of Belgrade, Dr Subotica 4, 11129 Belgrade, SerbiaDepartment of Research and Development, Institute of Virology, Vaccines and Sera-Torlak, Vojvode Stepe 458, 11152 Belgrade, SerbiaDepartment of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, SerbiaTo explore the potential usage of recombinant banana lectin as an immunostimulator it was of interest to analyze the effect it exerted in vivo in mice, upon two different routes of exposure. By Western blotting analysis of the gastric and intestinal luminal content it was found that recombinant banana lectin is stable in vivo in the mouse digestive tract, where it specifically interacts with the mucosal surfaces which can be inhibited by the addition of glucose. Its attachment to mice jejunum has been confirmed by immunofluorescence. In addition, it was able to induce systemic immunity, evidenced by specific antibody production, by oral application, a property that might be exploited for the induction of systemic immune responses.http://www.sciencedirect.com/science/article/pii/S1756464612000515Recombinant banana lectinMouse digestive tractAntibody subclass profile |
| spellingShingle | R. Dimitrijevic M. Stojanovic M. Micic Lj. Dimitrijevic M. Gavrovic-Jankulovic Recombinant banana lectin as mucosal immunostimulator Journal of Functional Foods Recombinant banana lectin Mouse digestive tract Antibody subclass profile |
| title | Recombinant banana lectin as mucosal immunostimulator |
| title_full | Recombinant banana lectin as mucosal immunostimulator |
| title_fullStr | Recombinant banana lectin as mucosal immunostimulator |
| title_full_unstemmed | Recombinant banana lectin as mucosal immunostimulator |
| title_short | Recombinant banana lectin as mucosal immunostimulator |
| title_sort | recombinant banana lectin as mucosal immunostimulator |
| topic | Recombinant banana lectin Mouse digestive tract Antibody subclass profile |
| url | http://www.sciencedirect.com/science/article/pii/S1756464612000515 |
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