Resolving the natural myocardial remodelling brought upon by cardiac contraction; a porcine ex-vivo cardiovascular magnetic resonance study of the left and right ventricle
Abstract Background The three-dimensional rearrangement of the right ventricular (RV) myocardium during cardiac deformation is unknown. Previous in-vivo studies have shown that myocardial left ventricular (LV) deformation is driven by rearrangement of aggregations of cardiomyocytes that can be chara...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-07-01
|
| Series: | Journal of Cardiovascular Magnetic Resonance |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12968-019-0547-2 |
| _version_ | 1797203709826957312 |
|---|---|
| author | Camilla Omann Peter Agger Nikolaj Bøgh Christoffer Laustsen Steffen Ringgaard Robert S. Stephenson Robert H. Anderson Vibeke E. Hjortdal Morten Smerup |
| author_facet | Camilla Omann Peter Agger Nikolaj Bøgh Christoffer Laustsen Steffen Ringgaard Robert S. Stephenson Robert H. Anderson Vibeke E. Hjortdal Morten Smerup |
| author_sort | Camilla Omann |
| collection | DOAJ |
| description | Abstract Background The three-dimensional rearrangement of the right ventricular (RV) myocardium during cardiac deformation is unknown. Previous in-vivo studies have shown that myocardial left ventricular (LV) deformation is driven by rearrangement of aggregations of cardiomyocytes that can be characterised by changes in the so-called E3-angle. Ex-vivo imaging offers superior spatial resolution compared with in-vivo measurements, and can thus provide novel insight into the deformation of the myocardial microstructure in both ventricles. This study sought to describe the dynamic changes of the orientations of the cardiomyocytes in both ventricles brought upon by cardiac contraction, with particular interest in the thin-walled RV, which has not previously been described in terms of its micro-architecture. Methods The hearts of 14 healthy 20 kg swine were excised and preserved in either a relaxed state or a contracted state. Myocardial architecture was assessed and compared between the two contractional states by quantification of the helical, transmural and E3-angles of the cardiomyocytes using high-resolution diffusion tensor imaging. Results The differences between the two states of contraction were most pronounced in the endocardium where the E3-angle decreased from 78.6° to 24.8° in the LV and from 82.6° to 68.6° in the RV. No significant change in neither the helical nor the transmural angle was found in the cardiomyocytes of the RV. In the endocardium of the LV, however, the helical angle increased from 35.4° to 47.8° and the transmural angle increased from 3.1° to 10.4°. Conclusion The entire myocardium rearranges through the cardiac cycle with the change in the orientation of the aggregations of cardiomyocytes being the predominant mediator of myocardial wall thickening. Interestingly, differences also exist between the RV and LV, which helps in the explanation of the different physiological capabilities of the ventricles. |
| first_indexed | 2024-04-24T08:23:39Z |
| format | Article |
| id | doaj.art-771a6609e09e41c2ab306f64c7e39f33 |
| institution | Directory Open Access Journal |
| issn | 1532-429X |
| language | English |
| last_indexed | 2024-04-24T08:23:39Z |
| publishDate | 2019-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Cardiovascular Magnetic Resonance |
| spelling | doaj.art-771a6609e09e41c2ab306f64c7e39f332024-04-16T23:31:12ZengElsevierJournal of Cardiovascular Magnetic Resonance1532-429X2019-07-0121111910.1186/s12968-019-0547-2Resolving the natural myocardial remodelling brought upon by cardiac contraction; a porcine ex-vivo cardiovascular magnetic resonance study of the left and right ventricleCamilla Omann0Peter Agger1Nikolaj Bøgh2Christoffer Laustsen3Steffen Ringgaard4Robert S. Stephenson5Robert H. Anderson6Vibeke E. Hjortdal7Morten Smerup8Department of Cardiothoracic & Vascular Surgery, Aarhus University HospitalDepartment of Cardiothoracic & Vascular Surgery, Aarhus University HospitalDepartment of Cardiothoracic & Vascular Surgery, Aarhus University HospitalDepartment of Clinical Medicine, Aarhus UniversityMR Research Centre, Aarhus UniversityDepartment of Clinical Medicine, Aarhus UniversityInstitute of Genetic Medicine, Newcastle UniversityDepartment of Cardiothoracic & Vascular Surgery, Aarhus University HospitalDepartment of Cardiothoracic & Vascular Surgery, Aarhus University HospitalAbstract Background The three-dimensional rearrangement of the right ventricular (RV) myocardium during cardiac deformation is unknown. Previous in-vivo studies have shown that myocardial left ventricular (LV) deformation is driven by rearrangement of aggregations of cardiomyocytes that can be characterised by changes in the so-called E3-angle. Ex-vivo imaging offers superior spatial resolution compared with in-vivo measurements, and can thus provide novel insight into the deformation of the myocardial microstructure in both ventricles. This study sought to describe the dynamic changes of the orientations of the cardiomyocytes in both ventricles brought upon by cardiac contraction, with particular interest in the thin-walled RV, which has not previously been described in terms of its micro-architecture. Methods The hearts of 14 healthy 20 kg swine were excised and preserved in either a relaxed state or a contracted state. Myocardial architecture was assessed and compared between the two contractional states by quantification of the helical, transmural and E3-angles of the cardiomyocytes using high-resolution diffusion tensor imaging. Results The differences between the two states of contraction were most pronounced in the endocardium where the E3-angle decreased from 78.6° to 24.8° in the LV and from 82.6° to 68.6° in the RV. No significant change in neither the helical nor the transmural angle was found in the cardiomyocytes of the RV. In the endocardium of the LV, however, the helical angle increased from 35.4° to 47.8° and the transmural angle increased from 3.1° to 10.4°. Conclusion The entire myocardium rearranges through the cardiac cycle with the change in the orientation of the aggregations of cardiomyocytes being the predominant mediator of myocardial wall thickening. Interestingly, differences also exist between the RV and LV, which helps in the explanation of the different physiological capabilities of the ventricles.http://link.springer.com/article/10.1186/s12968-019-0547-2MyocardiumDiffusion tensor imagingMicro-structureDynamic rearrangementMyocyte orientationMyocardial architecture |
| spellingShingle | Camilla Omann Peter Agger Nikolaj Bøgh Christoffer Laustsen Steffen Ringgaard Robert S. Stephenson Robert H. Anderson Vibeke E. Hjortdal Morten Smerup Resolving the natural myocardial remodelling brought upon by cardiac contraction; a porcine ex-vivo cardiovascular magnetic resonance study of the left and right ventricle Journal of Cardiovascular Magnetic Resonance Myocardium Diffusion tensor imaging Micro-structure Dynamic rearrangement Myocyte orientation Myocardial architecture |
| title | Resolving the natural myocardial remodelling brought upon by cardiac contraction; a porcine ex-vivo cardiovascular magnetic resonance study of the left and right ventricle |
| title_full | Resolving the natural myocardial remodelling brought upon by cardiac contraction; a porcine ex-vivo cardiovascular magnetic resonance study of the left and right ventricle |
| title_fullStr | Resolving the natural myocardial remodelling brought upon by cardiac contraction; a porcine ex-vivo cardiovascular magnetic resonance study of the left and right ventricle |
| title_full_unstemmed | Resolving the natural myocardial remodelling brought upon by cardiac contraction; a porcine ex-vivo cardiovascular magnetic resonance study of the left and right ventricle |
| title_short | Resolving the natural myocardial remodelling brought upon by cardiac contraction; a porcine ex-vivo cardiovascular magnetic resonance study of the left and right ventricle |
| title_sort | resolving the natural myocardial remodelling brought upon by cardiac contraction a porcine ex vivo cardiovascular magnetic resonance study of the left and right ventricle |
| topic | Myocardium Diffusion tensor imaging Micro-structure Dynamic rearrangement Myocyte orientation Myocardial architecture |
| url | http://link.springer.com/article/10.1186/s12968-019-0547-2 |
| work_keys_str_mv | AT camillaomann resolvingthenaturalmyocardialremodellingbroughtuponbycardiaccontractionaporcineexvivocardiovascularmagneticresonancestudyoftheleftandrightventricle AT peteragger resolvingthenaturalmyocardialremodellingbroughtuponbycardiaccontractionaporcineexvivocardiovascularmagneticresonancestudyoftheleftandrightventricle AT nikolajbøgh resolvingthenaturalmyocardialremodellingbroughtuponbycardiaccontractionaporcineexvivocardiovascularmagneticresonancestudyoftheleftandrightventricle AT christofferlaustsen resolvingthenaturalmyocardialremodellingbroughtuponbycardiaccontractionaporcineexvivocardiovascularmagneticresonancestudyoftheleftandrightventricle AT steffenringgaard resolvingthenaturalmyocardialremodellingbroughtuponbycardiaccontractionaporcineexvivocardiovascularmagneticresonancestudyoftheleftandrightventricle AT robertsstephenson resolvingthenaturalmyocardialremodellingbroughtuponbycardiaccontractionaporcineexvivocardiovascularmagneticresonancestudyoftheleftandrightventricle AT roberthanderson resolvingthenaturalmyocardialremodellingbroughtuponbycardiaccontractionaporcineexvivocardiovascularmagneticresonancestudyoftheleftandrightventricle AT vibekeehjortdal resolvingthenaturalmyocardialremodellingbroughtuponbycardiaccontractionaporcineexvivocardiovascularmagneticresonancestudyoftheleftandrightventricle AT mortensmerup resolvingthenaturalmyocardialremodellingbroughtuponbycardiaccontractionaporcineexvivocardiovascularmagneticresonancestudyoftheleftandrightventricle |