Quantitative evaluation of human sperm viability using MTT assay: A laboratory study
Background: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability. Objective: This study was performed to assess human sperm viability using MTT assay. Material...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shahid Sadoughi University of Medical Sciences
2020-11-01
|
| Series: | International Journal of Reproductive BioMedicine |
| Subjects: | |
| Online Access: | https://doi.org/10.18502/ijrm.v13i11.7966 |
| _version_ | 1819275525092278272 |
|---|---|
| author | Hamid Reza Momeni Mohammad Hussein Abnosi Najmeh Eskandari |
| author_facet | Hamid Reza Momeni Mohammad Hussein Abnosi Najmeh Eskandari |
| author_sort | Hamid Reza Momeni |
| collection | DOAJ |
| description | Background: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability.
Objective: This study was performed to assess human sperm viability using MTT assay.
Materials and Methods: In this laboratory study, human-ejaculated semen samples (n = 56 from different donors) were used. The sperm viability was determined using quantitative MTT assay and the sperm motility was assessed according to World Health Organization guidelines. Sperm viability and the correlation between sperm viability and motility were analyzed.
Results: Data revealed a marked positive correlation between MTT reduction rate and the percentage of viable spermatozoa. The Pearson's correlation coefficients also showed a significant correlation between sperm viability and motility.
Conclusion: MTT assay which is based on mitochondrial functionality is a reliable method for evaluating human sperm viability and could be used as a diagnostic test for predicting sperm fertilization ability in clinical settings. |
| first_indexed | 2024-12-23T23:25:42Z |
| format | Article |
| id | doaj.art-771b6d267d8240ceae6f703ab2d5cfd7 |
| institution | Directory Open Access Journal |
| issn | 2476-3772 |
| language | English |
| last_indexed | 2024-12-23T23:25:42Z |
| publishDate | 2020-11-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | International Journal of Reproductive BioMedicine |
| spelling | doaj.art-771b6d267d8240ceae6f703ab2d5cfd72022-12-21T17:26:14ZengShahid Sadoughi University of Medical SciencesInternational Journal of Reproductive BioMedicine2476-37722020-11-011898398810.18502/ijrm.v13i11.7966ijrm.v13i11.7966Quantitative evaluation of human sperm viability using MTT assay: A laboratory studyHamid Reza Momeni0Mohammad Hussein Abnosi1Najmeh Eskandari2Department of Biology, Faculty of Science, Arak University, Arak, Iran.Department of Biology, Faculty of Science, Arak University, Arak, Iran.Department of Biology, Faculty of Science, Arak University, Arak, Iran.Background: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability. Objective: This study was performed to assess human sperm viability using MTT assay. Materials and Methods: In this laboratory study, human-ejaculated semen samples (n = 56 from different donors) were used. The sperm viability was determined using quantitative MTT assay and the sperm motility was assessed according to World Health Organization guidelines. Sperm viability and the correlation between sperm viability and motility were analyzed. Results: Data revealed a marked positive correlation between MTT reduction rate and the percentage of viable spermatozoa. The Pearson's correlation coefficients also showed a significant correlation between sperm viability and motility. Conclusion: MTT assay which is based on mitochondrial functionality is a reliable method for evaluating human sperm viability and could be used as a diagnostic test for predicting sperm fertilization ability in clinical settings.https://doi.org/10.18502/ijrm.v13i11.7966human sperm, viability, mtt assay. |
| spellingShingle | Hamid Reza Momeni Mohammad Hussein Abnosi Najmeh Eskandari Quantitative evaluation of human sperm viability using MTT assay: A laboratory study International Journal of Reproductive BioMedicine human sperm, viability, mtt assay. |
| title | Quantitative evaluation of human sperm viability using MTT assay: A laboratory study |
| title_full | Quantitative evaluation of human sperm viability using MTT assay: A laboratory study |
| title_fullStr | Quantitative evaluation of human sperm viability using MTT assay: A laboratory study |
| title_full_unstemmed | Quantitative evaluation of human sperm viability using MTT assay: A laboratory study |
| title_short | Quantitative evaluation of human sperm viability using MTT assay: A laboratory study |
| title_sort | quantitative evaluation of human sperm viability using mtt assay a laboratory study |
| topic | human sperm, viability, mtt assay. |
| url | https://doi.org/10.18502/ijrm.v13i11.7966 |
| work_keys_str_mv | AT hamidrezamomeni quantitativeevaluationofhumanspermviabilityusingmttassayalaboratorystudy AT mohammadhusseinabnosi quantitativeevaluationofhumanspermviabilityusingmttassayalaboratorystudy AT najmeheskandari quantitativeevaluationofhumanspermviabilityusingmttassayalaboratorystudy |