Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes
Introduction: The ACE gene encodes the angiotensin-converting enzyme (ACE), which is characterized by the presence of allele I or the absence of allele D of a 287-bp fragment. The D allele is associated with higher ACE activity and increased angiotensin II levels and performance in strength-oriented...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Ilam University of Medical Sciences
2021-11-01
|
| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| Subjects: | |
| Online Access: | http://sjimu.medilam.ac.ir/article-1-7055-en.html |
| _version_ | 1819274922496622592 |
|---|---|
| author | Seyyed Rasoul Mousavi Mohammadreza Batavani Mohsen Ghofrani |
| author_facet | Seyyed Rasoul Mousavi Mohammadreza Batavani Mohsen Ghofrani |
| author_sort | Seyyed Rasoul Mousavi |
| collection | DOAJ |
| description | Introduction: The ACE gene encodes the angiotensin-converting enzyme (ACE), which is characterized by the presence of allele I or the absence of allele D of a 287-bp fragment. The D allele is associated with higher ACE activity and increased angiotensin II levels and performance in strength-oriented exercise tasks. This study aimed to compare the genetic profile of rs4646994 single nucleotide polymorphism of the ACE gene in the elite male weightlifters and non-athletes.
Material & Methods: In total, 30 elite male weightlifters (league, country, national team, Asian, world, and Olympic champions) living in Isfahan province along with 43 healthy non-athlete volunteers who were matched in age, height, and weight to the elite group formed the subjects of the study. After completing the consent form, saliva samples were collected from the participants, and after DNA extraction, ACE genotypes were determined using PCR-RLFP and electrophoresis techniques. Data analysis was performed using independent t-test, chi-square, and logistic regression in SPSS software (version 20).
(Ethic code: 7993)
Findings: The results showed that the prevalence of D allele in elite weightlifters and non-athletes was significantly higher than allele I in their groups (P<0.05). Moreover, in the genotyping distribution, D/I was most common both in the study population (7.50%), as well as weightlifters (7.56%) and non-athletes (5.46%). However, there was no significant difference in the elite weightlifters and non-athletes regarding the distribution of D/I ACE genotyping (χ2=0/31).
Discussion & Conclusion: The study showed a positive relationship between Iranian elite weightlifters and the ACE D allele, which probably distinguishes it as one of the effective genetic factors in creating a strength phenotype of Iranian weightlifters. |
| first_indexed | 2024-12-23T23:16:08Z |
| format | Article |
| id | doaj.art-7721d68f6ec3429c8f50758871806dc8 |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-12-23T23:16:08Z |
| publishDate | 2021-11-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-7721d68f6ec3429c8f50758871806dc82022-12-21T17:26:30ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352021-11-0129493102Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletesSeyyed Rasoul Mousavi0Mohammadreza Batavani1Mohsen Ghofrani2 MSc of Exercise Physiology, Department of Physical Education and Sport Science, Sistan and Baluchestan University, Sistan and Baluchestan, Iran Assistant Professor ,Center of Physical Education, Isfahan University of Technology, Isfahan 84156-83111, Iran Associate Professor, Department of Physical Education and Sport Sciences, Faculty of Physical Education and Sport Sciences, University of Sistan and Baluchestan, Sistan and Baluchestan, Iran Introduction: The ACE gene encodes the angiotensin-converting enzyme (ACE), which is characterized by the presence of allele I or the absence of allele D of a 287-bp fragment. The D allele is associated with higher ACE activity and increased angiotensin II levels and performance in strength-oriented exercise tasks. This study aimed to compare the genetic profile of rs4646994 single nucleotide polymorphism of the ACE gene in the elite male weightlifters and non-athletes. Material & Methods: In total, 30 elite male weightlifters (league, country, national team, Asian, world, and Olympic champions) living in Isfahan province along with 43 healthy non-athlete volunteers who were matched in age, height, and weight to the elite group formed the subjects of the study. After completing the consent form, saliva samples were collected from the participants, and after DNA extraction, ACE genotypes were determined using PCR-RLFP and electrophoresis techniques. Data analysis was performed using independent t-test, chi-square, and logistic regression in SPSS software (version 20). (Ethic code: 7993) Findings: The results showed that the prevalence of D allele in elite weightlifters and non-athletes was significantly higher than allele I in their groups (P<0.05). Moreover, in the genotyping distribution, D/I was most common both in the study population (7.50%), as well as weightlifters (7.56%) and non-athletes (5.46%). However, there was no significant difference in the elite weightlifters and non-athletes regarding the distribution of D/I ACE genotyping (χ2=0/31). Discussion & Conclusion: The study showed a positive relationship between Iranian elite weightlifters and the ACE D allele, which probably distinguishes it as one of the effective genetic factors in creating a strength phenotype of Iranian weightlifters.http://sjimu.medilam.ac.ir/article-1-7055-en.htmlalleleangiotensin-converting enzymeelite weightliftersmononucleotide polymorphism |
| spellingShingle | Seyyed Rasoul Mousavi Mohammadreza Batavani Mohsen Ghofrani Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām allele angiotensin-converting enzyme elite weightlifters mononucleotide polymorphism |
| title | Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes |
| title_full | Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes |
| title_fullStr | Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes |
| title_full_unstemmed | Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes |
| title_short | Comparison of the Genetic Profile of rs4646994 Single Nucleotide Polymorphism of the ACE Gene in the Elite Male Weightlifters and Non-athletes |
| title_sort | comparison of the genetic profile of rs4646994 single nucleotide polymorphism of the ace gene in the elite male weightlifters and non athletes |
| topic | allele angiotensin-converting enzyme elite weightlifters mononucleotide polymorphism |
| url | http://sjimu.medilam.ac.ir/article-1-7055-en.html |
| work_keys_str_mv | AT seyyedrasoulmousavi comparisonofthegeneticprofileofrs4646994singlenucleotidepolymorphismoftheacegeneintheelitemaleweightliftersandnonathletes AT mohammadrezabatavani comparisonofthegeneticprofileofrs4646994singlenucleotidepolymorphismoftheacegeneintheelitemaleweightliftersandnonathletes AT mohsenghofrani comparisonofthegeneticprofileofrs4646994singlenucleotidepolymorphismoftheacegeneintheelitemaleweightliftersandnonathletes |