Immunization with Dendritic Cells Pulsed ex vivo with Recombinant Chlamydial Protease-Like Activity Factor Induces Protective Immunity Against Genital Chlamydia muridarum Challenge
We have shown that immunization with soluble recombinant (r) chlamydial protease-like activity factor (rCPAF) and a T helper (Th) 1 type adjuvant can induce significantly enhanced bacterial clearance and protection against Chlamydia–induced pathological sequelae in the genital tract. In this study,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2011-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2011.00073/full |
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| author | Bernard eArulanandam Weidang eLi Ashlesh eMurthy Bharatkumar eChaganty M N Guentzel janakiram eseshu James Patrick Chambers Guangming eZhong |
| author_facet | Bernard eArulanandam Weidang eLi Ashlesh eMurthy Bharatkumar eChaganty M N Guentzel janakiram eseshu James Patrick Chambers Guangming eZhong |
| author_sort | Bernard eArulanandam |
| collection | DOAJ |
| description | We have shown that immunization with soluble recombinant (r) chlamydial protease-like activity factor (rCPAF) and a T helper (Th) 1 type adjuvant can induce significantly enhanced bacterial clearance and protection against Chlamydia–induced pathological sequelae in the genital tract. In this study, we investigated the use of bone marrow derived dendritic cells (BMDCs) pulsed ex vivo with rCPAF+CpG in an adoptive subcutaneous immunization for the ability to induce protective immunity against genital chlamydial infection. We found that BMDCs pulsed with rCPAF+CpG efficiently up-regulated the expression of activation markers CD86, CD80, CD40 and major histocompatibility complex class II (MHC II), and secreted interleukin-12, but not IL-10 and IL-4. Mice adoptively immunized with rCPAF+CpG-pulsed BMDCs or UV-EB+CpG-pulsed BMDCs produced elevated levels of antigen-specific IFN- and enhanced IgG1 and IgG2a antibodies. Moreover, mice immunized with rCPAF+CpG-pulsed BMDCs or UV-EB+CpG-pulsed BMDCs exhibited significantly reduced genital Chlamydia shedding, accelerated resolution of infection, and reduced oviduct pathology when compared to infected mock-immunized animals. These results suggest that adoptive subcutaneous immunization with ex vivo rCPAF-pulsed BMDCs is an effective approach, comparable to that induced by UV-EB-BMDCs, for inducing robust anti-Chlamydia immunity. |
| first_indexed | 2024-12-21T23:07:02Z |
| format | Article |
| id | doaj.art-7723cc81eff741629a0b57663da96e57 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-21T23:07:02Z |
| publishDate | 2011-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-7723cc81eff741629a0b57663da96e572022-12-21T18:47:09ZengFrontiers Media S.A.Frontiers in Immunology1664-32242011-12-01210.3389/fimmu.2011.0007316683Immunization with Dendritic Cells Pulsed ex vivo with Recombinant Chlamydial Protease-Like Activity Factor Induces Protective Immunity Against Genital Chlamydia muridarum ChallengeBernard eArulanandam0Weidang eLi1Ashlesh eMurthy2Bharatkumar eChaganty3M N Guentzel4janakiram eseshu5James Patrick Chambers6Guangming eZhong7University of Texas as San AntonioUniversity of Texas as San AntonioMidwestern UniversityUniversity of Texas as San AntonioUniversity of Texas as San AntonioUniversity of Texas as San AntonioUniversity of Texas as San AntonioUniversity of Texas Health Science CenterWe have shown that immunization with soluble recombinant (r) chlamydial protease-like activity factor (rCPAF) and a T helper (Th) 1 type adjuvant can induce significantly enhanced bacterial clearance and protection against Chlamydia–induced pathological sequelae in the genital tract. In this study, we investigated the use of bone marrow derived dendritic cells (BMDCs) pulsed ex vivo with rCPAF+CpG in an adoptive subcutaneous immunization for the ability to induce protective immunity against genital chlamydial infection. We found that BMDCs pulsed with rCPAF+CpG efficiently up-regulated the expression of activation markers CD86, CD80, CD40 and major histocompatibility complex class II (MHC II), and secreted interleukin-12, but not IL-10 and IL-4. Mice adoptively immunized with rCPAF+CpG-pulsed BMDCs or UV-EB+CpG-pulsed BMDCs produced elevated levels of antigen-specific IFN- and enhanced IgG1 and IgG2a antibodies. Moreover, mice immunized with rCPAF+CpG-pulsed BMDCs or UV-EB+CpG-pulsed BMDCs exhibited significantly reduced genital Chlamydia shedding, accelerated resolution of infection, and reduced oviduct pathology when compared to infected mock-immunized animals. These results suggest that adoptive subcutaneous immunization with ex vivo rCPAF-pulsed BMDCs is an effective approach, comparable to that induced by UV-EB-BMDCs, for inducing robust anti-Chlamydia immunity.http://journal.frontiersin.org/Journal/10.3389/fimmu.2011.00073/fullChlamydia trachomatisVaccinationCPAFDenditic CellsGenital Pathology |
| spellingShingle | Bernard eArulanandam Weidang eLi Ashlesh eMurthy Bharatkumar eChaganty M N Guentzel janakiram eseshu James Patrick Chambers Guangming eZhong Immunization with Dendritic Cells Pulsed ex vivo with Recombinant Chlamydial Protease-Like Activity Factor Induces Protective Immunity Against Genital Chlamydia muridarum Challenge Frontiers in Immunology Chlamydia trachomatis Vaccination CPAF Denditic Cells Genital Pathology |
| title | Immunization with Dendritic Cells Pulsed ex vivo with Recombinant Chlamydial Protease-Like Activity Factor Induces Protective Immunity Against Genital Chlamydia muridarum Challenge |
| title_full | Immunization with Dendritic Cells Pulsed ex vivo with Recombinant Chlamydial Protease-Like Activity Factor Induces Protective Immunity Against Genital Chlamydia muridarum Challenge |
| title_fullStr | Immunization with Dendritic Cells Pulsed ex vivo with Recombinant Chlamydial Protease-Like Activity Factor Induces Protective Immunity Against Genital Chlamydia muridarum Challenge |
| title_full_unstemmed | Immunization with Dendritic Cells Pulsed ex vivo with Recombinant Chlamydial Protease-Like Activity Factor Induces Protective Immunity Against Genital Chlamydia muridarum Challenge |
| title_short | Immunization with Dendritic Cells Pulsed ex vivo with Recombinant Chlamydial Protease-Like Activity Factor Induces Protective Immunity Against Genital Chlamydia muridarum Challenge |
| title_sort | immunization with dendritic cells pulsed ex vivo with recombinant chlamydial protease like activity factor induces protective immunity against genital chlamydia muridarum challenge |
| topic | Chlamydia trachomatis Vaccination CPAF Denditic Cells Genital Pathology |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2011.00073/full |
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