Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells
Pancreatic cancer usually has a poor prognosis, and no gene therapy has yet been developed that is effective to treat it. Since a unique characteristic of bone marrow-derived mesenchymal stem cells (MSCs) is that they migrate to tumor tissues, we wanted to determine whether MSCs could serve as a ve...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-03-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1422-0067/15/3/3729 |
| _version_ | 1811332882294112256 |
|---|---|
| author | Yun-Peng Sun Ben-Long Zhang Jian-Wen Duan Huan-Huan Wu Ben-Quan Wang Zheng-Ping Yu Wen-Jun Yang Yun-Feng Shan Meng-Tao Zhou Qi-Yu Zhang |
| author_facet | Yun-Peng Sun Ben-Long Zhang Jian-Wen Duan Huan-Huan Wu Ben-Quan Wang Zheng-Ping Yu Wen-Jun Yang Yun-Feng Shan Meng-Tao Zhou Qi-Yu Zhang |
| author_sort | Yun-Peng Sun |
| collection | DOAJ |
| description | Pancreatic cancer usually has a poor prognosis, and no gene therapy has yet been developed that is effective to treat it. Since a unique characteristic of bone marrow-derived mesenchymal stem cells (MSCs) is that they migrate to tumor tissues, we wanted to determine whether MSCs could serve as a vehicle of gene therapy for targeting pancreatic cancer. First, we successfully extracted MSCs from SD rats. Next, MSCs were efficiently transduced with NK4, an antagonist of hepatocyte growth factor (HGF) which comprising the N-terminal and the subsequent four kringle domains of HGF, by an adenoviral vector. Then, we confirmed that rat MSCs preferentially migrate to pancreatic cancer cells. Last, MSCs expressing NK4 (NK4-MSCs) strongly inhibited proliferation and migration of the pancreatic cancer cell line SW1990 after co-culture. These results indicate that MSCs can serve as a vehicle of gene therapy for targeting pancreatic cancer. |
| first_indexed | 2024-04-13T16:43:42Z |
| format | Article |
| id | doaj.art-77263ad082de4e4585695b80cecf879a |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T16:43:42Z |
| publishDate | 2014-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-77263ad082de4e4585695b80cecf879a2022-12-22T02:39:09ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-03-011533729374510.3390/ijms15033729ijms15033729Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer CellsYun-Peng Sun0Ben-Long Zhang1Jian-Wen Duan2Huan-Huan Wu3Ben-Quan Wang4Zheng-Ping Yu5Wen-Jun Yang6Yun-Feng Shan7Meng-Tao Zhou8Qi-Yu Zhang9Department of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, ChinaDepartment of General Surgery, Yiwu Chouzhou Hospital, Yiwu 322000, Zhejiang, ChinaDepartment of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, ChinaDepartment of Infectious Disease, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, ChinaDepartment of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, ChinaDepartment of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, ChinaDepartment of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, ChinaDepartment of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, ChinaDepartment of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, ChinaDepartment of Hepatobiliary Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325015, Zhejiang, ChinaPancreatic cancer usually has a poor prognosis, and no gene therapy has yet been developed that is effective to treat it. Since a unique characteristic of bone marrow-derived mesenchymal stem cells (MSCs) is that they migrate to tumor tissues, we wanted to determine whether MSCs could serve as a vehicle of gene therapy for targeting pancreatic cancer. First, we successfully extracted MSCs from SD rats. Next, MSCs were efficiently transduced with NK4, an antagonist of hepatocyte growth factor (HGF) which comprising the N-terminal and the subsequent four kringle domains of HGF, by an adenoviral vector. Then, we confirmed that rat MSCs preferentially migrate to pancreatic cancer cells. Last, MSCs expressing NK4 (NK4-MSCs) strongly inhibited proliferation and migration of the pancreatic cancer cell line SW1990 after co-culture. These results indicate that MSCs can serve as a vehicle of gene therapy for targeting pancreatic cancer.http://www.mdpi.com/1422-0067/15/3/3729NK4pancreatic cancerbone marrow-derived mesenchymal stem cellstransduction |
| spellingShingle | Yun-Peng Sun Ben-Long Zhang Jian-Wen Duan Huan-Huan Wu Ben-Quan Wang Zheng-Ping Yu Wen-Jun Yang Yun-Feng Shan Meng-Tao Zhou Qi-Yu Zhang Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells International Journal of Molecular Sciences NK4 pancreatic cancer bone marrow-derived mesenchymal stem cells transduction |
| title | Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells |
| title_full | Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells |
| title_fullStr | Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells |
| title_full_unstemmed | Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells |
| title_short | Effect of NK4 Transduction in Bone Marrow-Derived Mesenchymal Stem Cells on Biological Characteristics of Pancreatic Cancer Cells |
| title_sort | effect of nk4 transduction in bone marrow derived mesenchymal stem cells on biological characteristics of pancreatic cancer cells |
| topic | NK4 pancreatic cancer bone marrow-derived mesenchymal stem cells transduction |
| url | http://www.mdpi.com/1422-0067/15/3/3729 |
| work_keys_str_mv | AT yunpengsun effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells AT benlongzhang effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells AT jianwenduan effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells AT huanhuanwu effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells AT benquanwang effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells AT zhengpingyu effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells AT wenjunyang effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells AT yunfengshan effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells AT mengtaozhou effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells AT qiyuzhang effectofnk4transductioninbonemarrowderivedmesenchymalstemcellsonbiologicalcharacteristicsofpancreaticcancercells |