Degradation of keratin substrates by keratinolytic fungi
Background: The hydrolysis of keratin wastes by microorganisms is considered a biotechnological alternative for recycling and valorization through keratinolytic microorganisms. Despite their resistant structure, keratin wastes can be efficiently degraded by various microorganisms through the secreti...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-07-01
|
| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345817300295 |
| _version_ | 1818286303882510336 |
|---|---|
| author | Mariana Călin Diana Constantinescu-Aruxandei Elvira Alexandrescu Iuliana Răut Mihaela Badea Doni Melania-Liliana Arsene Florin Oancea Luiza Jecu Veronica Lazăr |
| author_facet | Mariana Călin Diana Constantinescu-Aruxandei Elvira Alexandrescu Iuliana Răut Mihaela Badea Doni Melania-Liliana Arsene Florin Oancea Luiza Jecu Veronica Lazăr |
| author_sort | Mariana Călin |
| collection | DOAJ |
| description | Background: The hydrolysis of keratin wastes by microorganisms is considered a biotechnological alternative for recycling and valorization through keratinolytic microorganisms. Despite their resistant structure, keratin wastes can be efficiently degraded by various microorganisms through the secretion of keratinases, which are promising enzymes for several applications, including detergents, fertilizers, and leather and textile industry. In an attempt to isolate keratinolytic microorganisms that can reach commercial exploitation as keratinase producers, the current work assesses the dynamics of keratin biodegradation by several keratinolytic fungal strains isolated from soil. The activity of fungal strains to degrade keratin substrates was evaluated by SEM, FTRIR-ATR spectra and TGA analysis.
Results: SEM observations offered relevant information on interactions between microorganism and structural elements of hair strands. FTIR spectra of the bands at 1035–1075 cm-1 assigned to sulfoxide bond appeared because of S–S bond breaking, which demonstrated the initiation of keratin biodegradation. According to TGA, in the second zone of thermal denaturation, where keratin degradation occurs, the highest weight loss of 71.10% was obtained for sample incubated with Fusarium sp. 1A.
Conclusions: Among the tested strains, Fusarium sp. 1A was the most active organism in the degradation process with the strongest denaturation of polypeptide chains. Because keratinolytic microorganisms and their enzymes keratinases represent a subject of scientific and economic interest because of their capability to hydrolyze keratin, Fusarium sp. 1A was selected for further studies. |
| first_indexed | 2024-12-13T01:22:27Z |
| format | Article |
| id | doaj.art-772817fc79d24d13b886c60238446048 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-13T01:22:27Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-772817fc79d24d13b886c602384460482022-12-22T00:04:12ZengElsevierElectronic Journal of Biotechnology0717-34582017-07-0128C10111210.1016/j.ejbt.2017.05.007Degradation of keratin substrates by keratinolytic fungiMariana Călin0Diana Constantinescu-Aruxandei1Elvira Alexandrescu2Iuliana Răut3Mihaela Badea Doni4Melania-Liliana Arsene5Florin Oancea6Luiza Jecu7Veronica Lazăr8Biotechnology Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Independentei Spl., 060021, Bucharest, RomaniaBiotechnology Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Independentei Spl., 060021, Bucharest, RomaniaBiotechnology Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Independentei Spl., 060021, Bucharest, RomaniaBiotechnology Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Independentei Spl., 060021, Bucharest, RomaniaBiotechnology Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Independentei Spl., 060021, Bucharest, RomaniaBiotechnology Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Independentei Spl., 060021, Bucharest, RomaniaBiotechnology Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Independentei Spl., 060021, Bucharest, RomaniaBiotechnology Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, 202 Independentei Spl., 060021, Bucharest, RomaniaFaculty of Biology, University of Bucharest, 91–95 Independentei Spl., Bucharest, RomaniaBackground: The hydrolysis of keratin wastes by microorganisms is considered a biotechnological alternative for recycling and valorization through keratinolytic microorganisms. Despite their resistant structure, keratin wastes can be efficiently degraded by various microorganisms through the secretion of keratinases, which are promising enzymes for several applications, including detergents, fertilizers, and leather and textile industry. In an attempt to isolate keratinolytic microorganisms that can reach commercial exploitation as keratinase producers, the current work assesses the dynamics of keratin biodegradation by several keratinolytic fungal strains isolated from soil. The activity of fungal strains to degrade keratin substrates was evaluated by SEM, FTRIR-ATR spectra and TGA analysis. Results: SEM observations offered relevant information on interactions between microorganism and structural elements of hair strands. FTIR spectra of the bands at 1035–1075 cm-1 assigned to sulfoxide bond appeared because of S–S bond breaking, which demonstrated the initiation of keratin biodegradation. According to TGA, in the second zone of thermal denaturation, where keratin degradation occurs, the highest weight loss of 71.10% was obtained for sample incubated with Fusarium sp. 1A. Conclusions: Among the tested strains, Fusarium sp. 1A was the most active organism in the degradation process with the strongest denaturation of polypeptide chains. Because keratinolytic microorganisms and their enzymes keratinases represent a subject of scientific and economic interest because of their capability to hydrolyze keratin, Fusarium sp. 1A was selected for further studies.http://www.sciencedirect.com/science/article/pii/S0717345817300295BiodegradationFTIRFungal speciesFusariumKeratin degradationKeratinasesKeratinolytic fungiSEMTGA |
| spellingShingle | Mariana Călin Diana Constantinescu-Aruxandei Elvira Alexandrescu Iuliana Răut Mihaela Badea Doni Melania-Liliana Arsene Florin Oancea Luiza Jecu Veronica Lazăr Degradation of keratin substrates by keratinolytic fungi Electronic Journal of Biotechnology Biodegradation FTIR Fungal species Fusarium Keratin degradation Keratinases Keratinolytic fungi SEM TGA |
| title | Degradation of keratin substrates by keratinolytic fungi |
| title_full | Degradation of keratin substrates by keratinolytic fungi |
| title_fullStr | Degradation of keratin substrates by keratinolytic fungi |
| title_full_unstemmed | Degradation of keratin substrates by keratinolytic fungi |
| title_short | Degradation of keratin substrates by keratinolytic fungi |
| title_sort | degradation of keratin substrates by keratinolytic fungi |
| topic | Biodegradation FTIR Fungal species Fusarium Keratin degradation Keratinases Keratinolytic fungi SEM TGA |
| url | http://www.sciencedirect.com/science/article/pii/S0717345817300295 |
| work_keys_str_mv | AT marianacalin degradationofkeratinsubstratesbykeratinolyticfungi AT dianaconstantinescuaruxandei degradationofkeratinsubstratesbykeratinolyticfungi AT elviraalexandrescu degradationofkeratinsubstratesbykeratinolyticfungi AT iulianaraut degradationofkeratinsubstratesbykeratinolyticfungi AT mihaelabadeadoni degradationofkeratinsubstratesbykeratinolyticfungi AT melanialilianaarsene degradationofkeratinsubstratesbykeratinolyticfungi AT florinoancea degradationofkeratinsubstratesbykeratinolyticfungi AT luizajecu degradationofkeratinsubstratesbykeratinolyticfungi AT veronicalazar degradationofkeratinsubstratesbykeratinolyticfungi |