The effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractility
Background/Aim. The fennel (Foeniculum vulgare Miller, Apiaceae) has a long history of use as traditional herb medicine due to its carminative properties. The study was aimed to investigate the effects of aqueous and methanol fennel stem extracts on intestinal activity. Methods. Relaxant activity of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Military Health Department, Ministry of Defance, Serbia
2018-01-01
|
| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2018/0042-84501600391G.pdf |
| _version_ | 1818111951881895936 |
|---|---|
| author | Gočmanac-Ignjatović Marija Kitić Dušanka Radenković Mirjana Kostić Milica Milutinović Milica Nedin-Ranković Gorana Branković Suzana |
| author_facet | Gočmanac-Ignjatović Marija Kitić Dušanka Radenković Mirjana Kostić Milica Milutinović Milica Nedin-Ranković Gorana Branković Suzana |
| author_sort | Gočmanac-Ignjatović Marija |
| collection | DOAJ |
| description | Background/Aim. The fennel (Foeniculum vulgare Miller, Apiaceae) has a long history of use as traditional herb medicine due to its carminative properties. The study was aimed to investigate the effects of aqueous and methanol fennel stem extracts on intestinal activity. Methods. Relaxant activity of aqueous and methanol fennel stem extracts was evaluated in vitro in three experimental models: spontaneous contraction, acetylcholine and potassium chloride (KCl)-induced contraction of an isolated rat ileum. The composition of aqueous and methanol fennel stem extracts was qualitatively analyzed using the high performance liquid chromatographic (HPLC) analysis. Results. In the presence of an aqueous fennel stem extract at a concentration of 3 mg/mL, the inhibition of the spontaneous contractions of isolated rat ileum was 35.05% ± 3.57%. In presence of a methanol fennel stem extract at the same concentration, the maximum reduction of the spontaneous contractions was 48.91% ± 6.31%. The extracts in a concentration- dependent manner significantly inhibited the acetylcholine and KCl induced contractions of the isolated rat ileum (p < 0.01). The following components were identified in fennel methanol stem extract: 3-caffeoylquinic acid, chlorogenic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, rutin, miquelianin, quercetin heterosides, 1,5-dicaffeoylquinic acid, 1,4-dicaffeoylquinic acid, apigenin and rosmarinic acid. In an aqueous extract, their presence is found in trace amounts. Conclusion. The results of this study showed that the aqueous and methanol fennel stem extracts have spasmolytic effects on the intestinal smooth muscle and may be used for the control of intestinal motility. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 46013 and Grant no. III 41018] |
| first_indexed | 2024-12-11T03:11:12Z |
| format | Article |
| id | doaj.art-77295893705f433da0b8a59ef7764119 |
| institution | Directory Open Access Journal |
| issn | 0042-8450 2406-0720 |
| language | English |
| last_indexed | 2024-12-11T03:11:12Z |
| publishDate | 2018-01-01 |
| publisher | Military Health Department, Ministry of Defance, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-77295893705f433da0b8a59ef77641192022-12-22T01:22:52ZengMilitary Health Department, Ministry of Defance, SerbiaVojnosanitetski Pregled0042-84502406-07202018-01-0175880981410.2298/VSP161001391G0042-84501600391GThe effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractilityGočmanac-Ignjatović Marija0Kitić Dušanka1Radenković Mirjana2Kostić Milica3Milutinović Milica4Nedin-Ranković Gorana5Branković Suzana6nemanemanemanemanemanemanemaBackground/Aim. The fennel (Foeniculum vulgare Miller, Apiaceae) has a long history of use as traditional herb medicine due to its carminative properties. The study was aimed to investigate the effects of aqueous and methanol fennel stem extracts on intestinal activity. Methods. Relaxant activity of aqueous and methanol fennel stem extracts was evaluated in vitro in three experimental models: spontaneous contraction, acetylcholine and potassium chloride (KCl)-induced contraction of an isolated rat ileum. The composition of aqueous and methanol fennel stem extracts was qualitatively analyzed using the high performance liquid chromatographic (HPLC) analysis. Results. In the presence of an aqueous fennel stem extract at a concentration of 3 mg/mL, the inhibition of the spontaneous contractions of isolated rat ileum was 35.05% ± 3.57%. In presence of a methanol fennel stem extract at the same concentration, the maximum reduction of the spontaneous contractions was 48.91% ± 6.31%. The extracts in a concentration- dependent manner significantly inhibited the acetylcholine and KCl induced contractions of the isolated rat ileum (p < 0.01). The following components were identified in fennel methanol stem extract: 3-caffeoylquinic acid, chlorogenic acid, 4-caffeoylquinic acid, 1,3-dicaffeoylquinic acid, rutin, miquelianin, quercetin heterosides, 1,5-dicaffeoylquinic acid, 1,4-dicaffeoylquinic acid, apigenin and rosmarinic acid. In an aqueous extract, their presence is found in trace amounts. Conclusion. The results of this study showed that the aqueous and methanol fennel stem extracts have spasmolytic effects on the intestinal smooth muscle and may be used for the control of intestinal motility. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 46013 and Grant no. III 41018]http://www.doiserbia.nb.rs/img/doi/0042-8450/2018/0042-84501600391G.pdfileumratsfoeniculumacetylcholinepotassium chloridetreatment outcome |
| spellingShingle | Gočmanac-Ignjatović Marija Kitić Dušanka Radenković Mirjana Kostić Milica Milutinović Milica Nedin-Ranković Gorana Branković Suzana The effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractility Vojnosanitetski Pregled ileum rats foeniculum acetylcholine potassium chloride treatment outcome |
| title | The effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractility |
| title_full | The effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractility |
| title_fullStr | The effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractility |
| title_full_unstemmed | The effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractility |
| title_short | The effect of the aqueous and methanol fennel stem extracts (Foeniculum vulgare Miller) on isolated rat ileum contractility |
| title_sort | effect of the aqueous and methanol fennel stem extracts foeniculum vulgare miller on isolated rat ileum contractility |
| topic | ileum rats foeniculum acetylcholine potassium chloride treatment outcome |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2018/0042-84501600391G.pdf |
| work_keys_str_mv | AT gocmanacignjatovicmarija theeffectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT kiticdusanka theeffectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT radenkovicmirjana theeffectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT kosticmilica theeffectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT milutinovicmilica theeffectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT nedinrankovicgorana theeffectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT brankovicsuzana theeffectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT gocmanacignjatovicmarija effectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT kiticdusanka effectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT radenkovicmirjana effectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT kosticmilica effectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT milutinovicmilica effectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT nedinrankovicgorana effectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility AT brankovicsuzana effectoftheaqueousandmethanolfennelstemextractsfoeniculumvulgaremilleronisolatedratileumcontractility |