Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118
Acinetobacter baumannii A118, a strain isolated from the blood of an infected patient, is naturally competent and unlike most clinical strains, is susceptible to a variety of different antibiotics including those usually used for selection in genetic manipulations. These characteristics make strain...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01599/full |
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| author | Maria S. Ramirez William F. Penwell German M. Traglia Daniel L. Zimbler Jennifer A. Gaddy Jennifer A. Gaddy Nikolas Nikolaidis Brock A. Arivett Mark D. Adams Robert A. Bonomo Luis A. Actis Marcelo E. Tolmasky |
| author_facet | Maria S. Ramirez William F. Penwell German M. Traglia Daniel L. Zimbler Jennifer A. Gaddy Jennifer A. Gaddy Nikolas Nikolaidis Brock A. Arivett Mark D. Adams Robert A. Bonomo Luis A. Actis Marcelo E. Tolmasky |
| author_sort | Maria S. Ramirez |
| collection | DOAJ |
| description | Acinetobacter baumannii A118, a strain isolated from the blood of an infected patient, is naturally competent and unlike most clinical strains, is susceptible to a variety of different antibiotics including those usually used for selection in genetic manipulations. These characteristics make strain A118 a convenient model for genetic studies of A. baumannii. To identify potential virulence factors, its complete genome was analyzed and compared to other A. baumannii genomes. A. baumannii A118 includes gene clusters coding for the acinetobactin and baumannoferrin iron acquisition systems. Iron-regulated expression of the BauA outer membrane receptor for ferric-acinetobactin complexes was confirmed as well as the utilization of acinetobactin. A. baumannii A118 also possesses the feoABC genes, which code for the main bacterial ferrous uptake system. The functionality of baumannoferrin was suggested by the ability of A. baumannii A118 culture supernatants to cross feed an indicator BauA-deficient strain plated on iron-limiting media. A. baumannii A118 behaved as non-motile but included the csuA/BABCDE chaperone-usher pilus assembly operon and produced biofilms on polystyrene and glass surfaces. While a known capsular polysaccharide (K) locus was identified, the outer core polysaccharide (OC) locus, which belongs to group B, showed differences with available sequences. Our results show that despite being susceptible to most antibiotics, strain A118 conserves known virulence-related traits enhancing its value as model to study A. baumannii pathogenicity. |
| first_indexed | 2024-12-14T18:42:17Z |
| format | Article |
| id | doaj.art-772963d8fbc340d0964b517251fc4197 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T18:42:17Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-772963d8fbc340d0964b517251fc41972022-12-21T22:51:28ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-07-011010.3389/fmicb.2019.01599457645Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118Maria S. Ramirez0William F. Penwell1German M. Traglia2Daniel L. Zimbler3Jennifer A. Gaddy4Jennifer A. Gaddy5Nikolas Nikolaidis6Brock A. Arivett7Mark D. Adams8Robert A. Bonomo9Luis A. Actis10Marcelo E. Tolmasky11Department of Biological Science, Center for Applied Biotechnology Studies, California State University, Fullerton, Fullerton, CA, United StatesDepartment of Microbiology, Miami University, Oxford, OH, United StatesLaboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Hospital de Clínicas “José de San Martín”, Buenos Aires, ArgentinaDepartment of Microbiology, Miami University, Oxford, OH, United StatesDepartment of Microbiology, Miami University, Oxford, OH, United StatesDepartment of Medicine, Vanderbilt University Medical Center, Nashville, TN, United StatesDepartment of Biological Science, Center for Applied Biotechnology Studies, California State University, Fullerton, Fullerton, CA, United StatesDepartment of Microbiology, Miami University, Oxford, OH, United StatesDepartment of Genetics, School of Medicine, Case Western Reserve University, Cleveland, OH, United StatesDepartments of Pharmacology and Molecular Biology and Microbiology, Louis Stokes Cleveland Veterans Affairs Medical Center, School of Medicine, Case Western Reserve University, Cleveland, OH, United StatesDepartment of Microbiology, Miami University, Oxford, OH, United StatesDepartment of Biological Science, Center for Applied Biotechnology Studies, California State University, Fullerton, Fullerton, CA, United StatesAcinetobacter baumannii A118, a strain isolated from the blood of an infected patient, is naturally competent and unlike most clinical strains, is susceptible to a variety of different antibiotics including those usually used for selection in genetic manipulations. These characteristics make strain A118 a convenient model for genetic studies of A. baumannii. To identify potential virulence factors, its complete genome was analyzed and compared to other A. baumannii genomes. A. baumannii A118 includes gene clusters coding for the acinetobactin and baumannoferrin iron acquisition systems. Iron-regulated expression of the BauA outer membrane receptor for ferric-acinetobactin complexes was confirmed as well as the utilization of acinetobactin. A. baumannii A118 also possesses the feoABC genes, which code for the main bacterial ferrous uptake system. The functionality of baumannoferrin was suggested by the ability of A. baumannii A118 culture supernatants to cross feed an indicator BauA-deficient strain plated on iron-limiting media. A. baumannii A118 behaved as non-motile but included the csuA/BABCDE chaperone-usher pilus assembly operon and produced biofilms on polystyrene and glass surfaces. While a known capsular polysaccharide (K) locus was identified, the outer core polysaccharide (OC) locus, which belongs to group B, showed differences with available sequences. Our results show that despite being susceptible to most antibiotics, strain A118 conserves known virulence-related traits enhancing its value as model to study A. baumannii pathogenicity.https://www.frontiersin.org/article/10.3389/fmicb.2019.01599/fullAcinetobactervirulence factorspathogenicityhospital infectioncommunity infectionESKAPE |
| spellingShingle | Maria S. Ramirez William F. Penwell German M. Traglia Daniel L. Zimbler Jennifer A. Gaddy Jennifer A. Gaddy Nikolas Nikolaidis Brock A. Arivett Mark D. Adams Robert A. Bonomo Luis A. Actis Marcelo E. Tolmasky Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118 Frontiers in Microbiology Acinetobacter virulence factors pathogenicity hospital infection community infection ESKAPE |
| title | Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118 |
| title_full | Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118 |
| title_fullStr | Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118 |
| title_full_unstemmed | Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118 |
| title_short | Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118 |
| title_sort | identification of potential virulence factors in the model strain acinetobacter baumannii a118 |
| topic | Acinetobacter virulence factors pathogenicity hospital infection community infection ESKAPE |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01599/full |
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