Progressive Rearrangement of Telomeric Sequences Added to Both the ITR Ends of the Yeast Linear pGKL Plasmid
<p>Relocation into the nucleus of the yeast cytoplasmic linear plasmids was studied using a monitor plasmid pCLU1. In <it>Saccharomyces cerevisiae</it>, the nuclearly-relocated pCLU1 replicated in a linear form (termed pTLU-type plasmid) which carried the host telomeric repeats TG&...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2003-01-01
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| Series: | Biological Procedures Online |
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| Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/44/m44.htm |
| _version_ | 1818229075418808320 |
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| author | Gunge Norio Takata Hideki Matsuura Akira Fukuda Kohsai |
| author_facet | Gunge Norio Takata Hideki Matsuura Akira Fukuda Kohsai |
| author_sort | Gunge Norio |
| collection | DOAJ |
| description | <p>Relocation into the nucleus of the yeast cytoplasmic linear plasmids was studied using a monitor plasmid pCLU1. In <it>Saccharomyces cerevisiae</it>, the nuclearly-relocated pCLU1 replicated in a linear form (termed pTLU-type plasmid) which carried the host telomeric repeats TG<sub>1-3</sub> of 300-350 bp at both ends. The telomere sequences mainly consisted of a major motif TGTGTGGGTGTGG which was complementary to part of the RNA template of yeast telomerase and were directly added to the very end of the pCLU1-terminal element ITR (inverted terminal repeat), suggesting that the ITR end played a role as a substrate of telomerase. The telomere sequences varied among isolated pTLU-type plasmids, but the TG<sub>1-3</sub> organization was symmetrically identical on both ends of any one plasmid. During cell growth under non-selective condition, the telomeric repeat sequences were progressively rearranged on one side, but not on the opposite side of pTLU plasmid ends. This indicates that the mode of telomeric DNA replication or repair differed between both ends. Clonal analysis showed that the intense rearrangement of telomeric DNA was closely associated with extreme instability of pTLU plasmids. |
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| id | doaj.art-772a3fb76e734da599faa948dde6995f |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-12T10:12:50Z |
| publishDate | 2003-01-01 |
| publisher | BMC |
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| series | Biological Procedures Online |
| spelling | doaj.art-772a3fb76e734da599faa948dde6995f2022-12-22T00:27:45ZengBMCBiological Procedures Online1480-92222003-01-0151294210.1251/bpo44Progressive Rearrangement of Telomeric Sequences Added to Both the ITR Ends of the Yeast Linear pGKL PlasmidGunge NorioTakata HidekiMatsuura AkiraFukuda Kohsai<p>Relocation into the nucleus of the yeast cytoplasmic linear plasmids was studied using a monitor plasmid pCLU1. In <it>Saccharomyces cerevisiae</it>, the nuclearly-relocated pCLU1 replicated in a linear form (termed pTLU-type plasmid) which carried the host telomeric repeats TG<sub>1-3</sub> of 300-350 bp at both ends. The telomere sequences mainly consisted of a major motif TGTGTGGGTGTGG which was complementary to part of the RNA template of yeast telomerase and were directly added to the very end of the pCLU1-terminal element ITR (inverted terminal repeat), suggesting that the ITR end played a role as a substrate of telomerase. The telomere sequences varied among isolated pTLU-type plasmids, but the TG<sub>1-3</sub> organization was symmetrically identical on both ends of any one plasmid. During cell growth under non-selective condition, the telomeric repeat sequences were progressively rearranged on one side, but not on the opposite side of pTLU plasmid ends. This indicates that the mode of telomeric DNA replication or repair differed between both ends. Clonal analysis showed that the intense rearrangement of telomeric DNA was closely associated with extreme instability of pTLU plasmids.http://www.biologicalprocedures.com/bpo/arts/1/44/m44.htmtelomeretelomeraseplasmids |
| spellingShingle | Gunge Norio Takata Hideki Matsuura Akira Fukuda Kohsai Progressive Rearrangement of Telomeric Sequences Added to Both the ITR Ends of the Yeast Linear pGKL Plasmid Biological Procedures Online telomere telomerase plasmids |
| title | Progressive Rearrangement of Telomeric Sequences Added to Both the ITR Ends of the Yeast Linear pGKL Plasmid |
| title_full | Progressive Rearrangement of Telomeric Sequences Added to Both the ITR Ends of the Yeast Linear pGKL Plasmid |
| title_fullStr | Progressive Rearrangement of Telomeric Sequences Added to Both the ITR Ends of the Yeast Linear pGKL Plasmid |
| title_full_unstemmed | Progressive Rearrangement of Telomeric Sequences Added to Both the ITR Ends of the Yeast Linear pGKL Plasmid |
| title_short | Progressive Rearrangement of Telomeric Sequences Added to Both the ITR Ends of the Yeast Linear pGKL Plasmid |
| title_sort | progressive rearrangement of telomeric sequences added to both the itr ends of the yeast linear pgkl plasmid |
| topic | telomere telomerase plasmids |
| url | http://www.biologicalprocedures.com/bpo/arts/1/44/m44.htm |
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