Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers
Genetic diversity assessment is the preliminary work for the development of variety and conservation of diversity. Finger millet is a very important crop in Nepal however, its genetic potential has not been fully utilized. Genetic diversity was assessed in forty landraces of finger millet using 9 RA...
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| Format: | Article |
| Language: | English |
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Biotechnology Society of Nepal
2020-07-01
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| Series: | Nepal Journal of Biotechnology |
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| Online Access: | https://www.nepjol.info/index.php/NJB/article/view/30204 |
| _version_ | 1818416765465526272 |
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| author | Bal Krishna Joshi Darbin Joshi Surya Kanta Ghimire |
| author_facet | Bal Krishna Joshi Darbin Joshi Surya Kanta Ghimire |
| author_sort | Bal Krishna Joshi |
| collection | DOAJ |
| description | Genetic diversity assessment is the preliminary work for the development of variety and conservation of diversity. Finger millet is a very important crop in Nepal however, its genetic potential has not been fully utilized. Genetic diversity was assessed in forty landraces of finger millet using 9 RAPD and 5 SSR markers. These landraces were collected from Kaski and Dhading districts. None of single primers of these RAPD and SSR could separate all 40 landraces. The average number of bands were 6.33 and 7.8 per RAPD and SSR primers respectively. Mean polymorphism information content was of 0.314 for RAPD and 0.37 for SSR. Primer OPA-4 produced the highest number of bands and the lowest numbers of bands were produced by OPA-16. Among the SSR primers, SSR-06 produced the highest number of polymorphic bands and UGEP-53 produced the lowest bands. RAPD based dendrogram has generated four clusters and SSR based dendrogram has generated two clusters. In both dendrogram and principal component analyses, Purbeli landrace was found unique locating separately in the cluster and scatter plot. Nei's genetic distance produced by RAPD and SSR primers was similar that is 0.327 by RAPD and 0.296 by SSR markers. Genetic distance produced by SSR markers was higher than distance produced by RAPD marker. These landraces were from two districts and therefore have shown intermediate diversity. These molecular marker-based findings should would be more useful if we could link with agromorphological traits. Inclusion of large number of landraces collected from different areas are required to get higher level diversity in addition to associate genetic diversity with geographical sites. Groupings of these landraces could be useful for selecting landraces in breeding program as well as planning conservation program. |
| first_indexed | 2024-12-14T11:56:05Z |
| format | Article |
| id | doaj.art-772c2798fdea4d51a338bd4cfa82b93c |
| institution | Directory Open Access Journal |
| issn | 2091-1130 2467-9313 |
| language | English |
| last_indexed | 2024-12-14T11:56:05Z |
| publishDate | 2020-07-01 |
| publisher | Biotechnology Society of Nepal |
| record_format | Article |
| series | Nepal Journal of Biotechnology |
| spelling | doaj.art-772c2798fdea4d51a338bd4cfa82b93c2022-12-21T23:02:06ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132020-07-018111110.3126/njb.v8i1.30204Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR MarkersBal Krishna Joshi0https://orcid.org/0000-0002-7848-5824Darbin Joshi1Surya Kanta Ghimire2National Agriculture Genetic Resources Center, Khumaltar, Kathmandu, NepalCIMMYT, Kathmandu, NepalAgriculture and Forestry University, Rampur, Chitwan, NepalGenetic diversity assessment is the preliminary work for the development of variety and conservation of diversity. Finger millet is a very important crop in Nepal however, its genetic potential has not been fully utilized. Genetic diversity was assessed in forty landraces of finger millet using 9 RAPD and 5 SSR markers. These landraces were collected from Kaski and Dhading districts. None of single primers of these RAPD and SSR could separate all 40 landraces. The average number of bands were 6.33 and 7.8 per RAPD and SSR primers respectively. Mean polymorphism information content was of 0.314 for RAPD and 0.37 for SSR. Primer OPA-4 produced the highest number of bands and the lowest numbers of bands were produced by OPA-16. Among the SSR primers, SSR-06 produced the highest number of polymorphic bands and UGEP-53 produced the lowest bands. RAPD based dendrogram has generated four clusters and SSR based dendrogram has generated two clusters. In both dendrogram and principal component analyses, Purbeli landrace was found unique locating separately in the cluster and scatter plot. Nei's genetic distance produced by RAPD and SSR primers was similar that is 0.327 by RAPD and 0.296 by SSR markers. Genetic distance produced by SSR markers was higher than distance produced by RAPD marker. These landraces were from two districts and therefore have shown intermediate diversity. These molecular marker-based findings should would be more useful if we could link with agromorphological traits. Inclusion of large number of landraces collected from different areas are required to get higher level diversity in addition to associate genetic diversity with geographical sites. Groupings of these landraces could be useful for selecting landraces in breeding program as well as planning conservation program.https://www.nepjol.info/index.php/NJB/article/view/30204finger millet landracesdna finger printgenetic divesitydna marker |
| spellingShingle | Bal Krishna Joshi Darbin Joshi Surya Kanta Ghimire Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers Nepal Journal of Biotechnology finger millet landraces dna finger print genetic divesity dna marker |
| title | Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers |
| title_full | Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers |
| title_fullStr | Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers |
| title_full_unstemmed | Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers |
| title_short | Genetic Diversity in Finger Millet Landraces revealed by RAPD and SSR Markers |
| title_sort | genetic diversity in finger millet landraces revealed by rapd and ssr markers |
| topic | finger millet landraces dna finger print genetic divesity dna marker |
| url | https://www.nepjol.info/index.php/NJB/article/view/30204 |
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