Chronic physical stress changes gene expression of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats
In this study we examined how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes and cAMP response element-binding (CREB) in the adrenal medulla and the weight of adrenal glands of rats. Also, we examined how CFR and additional acute immobilization stress a...
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| Format: | Article |
| Language: | English |
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Sciendo
2012-01-01
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| Series: | Acta Veterinaria |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0567-8315/2012/0567-83151203151G.pdf |
| _version_ | 1818599771443560448 |
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| author | Gavrilović Ljubica Stojiljković Vesna Kasapović Jelena Pejić Snežana Todorović Ana Pajović Snežana B. Dronjak Slađana |
| author_facet | Gavrilović Ljubica Stojiljković Vesna Kasapović Jelena Pejić Snežana Todorović Ana Pajović Snežana B. Dronjak Slađana |
| author_sort | Gavrilović Ljubica |
| collection | DOAJ |
| description | In this study we examined how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes and cAMP response element-binding (CREB) in the adrenal medulla and the weight of adrenal glands of rats. Also, we examined how CFR and additional acute immobilization stress affect the expression of catecholamine biosynthetic enzymes in the adrenal medulla and the concentration of catecholamines and corticosterone (CORT) in the blood plasma. In this experiment we used as a model forced exercise in rats (treadmill running). We used the most advanced method for determining the level of gene expression, Real-time PCR with TaqMan probes, as well as Western blot analysis (ECL). We found that CFR decreases tyrosine hydroxylase (TH), and dopamine-β-hydroxylase (DBH) mRNA and protein levels in the adrenal medulla. The decreased TH and DBH mRNA levels coincide with the reduced expression of CREB in the adrenal medulla and with the reduced plasma CORT level. Additionally, CFR reduces the level of phenylethanolamine N-methyltransferase (PNMT) mRNA, but elevates its protein level in the adrenal medulla and increases the concentration of adrenaline (A) in the plasma. Reduced level of PNMT mRNA in the adrenal medulla coincides with reduced plasma CORT level. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. The increased synthesis of PNMT enzyme in the adrenal medulla may result in an increased biosynthesis of A under chronic stress conditions. Additionally, increased level of catecholamines in the plasma after chronic physical stress is the allostatic load that may induce numerous diseases and pathological conditions. |
| first_indexed | 2024-12-16T12:24:53Z |
| format | Article |
| id | doaj.art-772e5f6fc8fc4707a7d7390f90bb6a35 |
| institution | Directory Open Access Journal |
| issn | 0567-8315 1820-7448 |
| language | English |
| last_indexed | 2024-12-16T12:24:53Z |
| publishDate | 2012-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj.art-772e5f6fc8fc4707a7d7390f90bb6a352022-12-21T22:31:51ZengSciendoActa Veterinaria0567-83151820-74482012-01-01622-315116910.2298/AVB1203151GChronic physical stress changes gene expression of catecholamine biosynthetic enzymes in the adrenal medulla of adult ratsGavrilović LjubicaStojiljković VesnaKasapović JelenaPejić SnežanaTodorović AnaPajović Snežana B.Dronjak SlađanaIn this study we examined how chronic forced running (CFR) affects the expression of catecholamine biosynthetic enzymes and cAMP response element-binding (CREB) in the adrenal medulla and the weight of adrenal glands of rats. Also, we examined how CFR and additional acute immobilization stress affect the expression of catecholamine biosynthetic enzymes in the adrenal medulla and the concentration of catecholamines and corticosterone (CORT) in the blood plasma. In this experiment we used as a model forced exercise in rats (treadmill running). We used the most advanced method for determining the level of gene expression, Real-time PCR with TaqMan probes, as well as Western blot analysis (ECL). We found that CFR decreases tyrosine hydroxylase (TH), and dopamine-β-hydroxylase (DBH) mRNA and protein levels in the adrenal medulla. The decreased TH and DBH mRNA levels coincide with the reduced expression of CREB in the adrenal medulla and with the reduced plasma CORT level. Additionally, CFR reduces the level of phenylethanolamine N-methyltransferase (PNMT) mRNA, but elevates its protein level in the adrenal medulla and increases the concentration of adrenaline (A) in the plasma. Reduced level of PNMT mRNA in the adrenal medulla coincides with reduced plasma CORT level. The additional acute immobilization stress increases gene expression of catecholamine biosynthetic enzymes in the adrenal medulla, as well as catecholamines and CORT levels in the plasma. The increased synthesis of PNMT enzyme in the adrenal medulla may result in an increased biosynthesis of A under chronic stress conditions. Additionally, increased level of catecholamines in the plasma after chronic physical stress is the allostatic load that may induce numerous diseases and pathological conditions.http://www.doiserbia.nb.rs/img/doi/0567-8315/2012/0567-83151203151G.pdfacute immobilization stressadrenal medullacatecholaminechronic stressgene expression |
| spellingShingle | Gavrilović Ljubica Stojiljković Vesna Kasapović Jelena Pejić Snežana Todorović Ana Pajović Snežana B. Dronjak Slađana Chronic physical stress changes gene expression of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats Acta Veterinaria acute immobilization stress adrenal medulla catecholamine chronic stress gene expression |
| title | Chronic physical stress changes gene expression of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats |
| title_full | Chronic physical stress changes gene expression of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats |
| title_fullStr | Chronic physical stress changes gene expression of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats |
| title_full_unstemmed | Chronic physical stress changes gene expression of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats |
| title_short | Chronic physical stress changes gene expression of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats |
| title_sort | chronic physical stress changes gene expression of catecholamine biosynthetic enzymes in the adrenal medulla of adult rats |
| topic | acute immobilization stress adrenal medulla catecholamine chronic stress gene expression |
| url | http://www.doiserbia.nb.rs/img/doi/0567-8315/2012/0567-83151203151G.pdf |
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