Acute human bocavirus 1 infection in child with life-threatening bilateral bronchiolitis and right-sided pneumonia: a case report
Abstract Background Human bocavirus 1 is a commonly detected human parvovirus. Many studies have shown human bocavirus 1 as a pathogen in association with acute respiratory tract infections in children. However, because human bocavirus 1 persists in the upper airways for extensive time periods after...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-09-01
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| Series: | Journal of Medical Case Reports |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13256-019-2222-5 |
| _version_ | 1828395089203822592 |
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| author | Inga Ziemele Man Xu Anda Vilmane Santa Rasa-Dzelzkaleja Lea Hedman Klaus Hedman Maria Söderlund-Venermo Zaiga Nora-Krukle Modra Murovska Dace Gardovska |
| author_facet | Inga Ziemele Man Xu Anda Vilmane Santa Rasa-Dzelzkaleja Lea Hedman Klaus Hedman Maria Söderlund-Venermo Zaiga Nora-Krukle Modra Murovska Dace Gardovska |
| author_sort | Inga Ziemele |
| collection | DOAJ |
| description | Abstract Background Human bocavirus 1 is a commonly detected human parvovirus. Many studies have shown human bocavirus 1 as a pathogen in association with acute respiratory tract infections in children. However, because human bocavirus 1 persists in the upper airways for extensive time periods after acute infection, the definition and diagnostics of acute human bocavirus 1 infection is challenging. Until now, detection of human bocavirus 1 exclusively, high viral load in respiratory samples, and viremia have been associated with a clinical picture of acute respiratory illness. There are no studies showing detection of human bocavirus 1 messenger ribonucleic acid in the peripheral blood mononuclear cells as a diagnostic marker for acute lower respiratory tract infection. Case presentation We report the case of a 17-month-old Latvian boy who presented in intensive care unit with acute bilateral bronchiolitis, with a history of rhinorrhea and cough for 6 days and fever for the last 2 days prior to admission, followed by severe respiratory distress and tracheal intubation. Human bocavirus 1 was the only respiratory virus detected by a qualitative multiplex polymerase chain reaction panel. For the diagnosis of acute human bocavirus 1 infection, both molecular and serological approaches were used. Human bocavirus 1 deoxyribonucleic acid (DNA) was detected simultaneously in nasopharyngeal aspirate, stool, and blood, as well as in the corresponding cell-free blood plasma by qualitative and quantitative polymerase chain reaction, revealing high DNA-copy numbers in nasopharyngeal aspirate and stool. Despite a low-load viremia, human bocavirus 1 messenger ribonucleic acid was found in the peripheral blood mononuclear cells. For detection of human bocavirus 1-specific antibodies, non-competitive immunoglobulin M and competitive immunoglobulin G enzyme immunoassays were used. The plasma was positive for both human bocavirus 1-specific immunoglobulin M and immunoglobulin G antibodies. Conclusions The presence of human bocavirus 1 genomic DNA in blood plasma and human bocavirus 1 messenger ribonucleic acid in peripheral blood mononuclear cells together with human bocavirus 1-specific immunoglobulin M are markers of acute human bocavirus 1 infection that may cause life-threatening acute bronchiolitis. |
| first_indexed | 2024-12-10T08:05:37Z |
| format | Article |
| id | doaj.art-77357152ea834710959522c56406c9ad |
| institution | Directory Open Access Journal |
| issn | 1752-1947 |
| language | English |
| last_indexed | 2024-12-10T08:05:37Z |
| publishDate | 2019-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Medical Case Reports |
| spelling | doaj.art-77357152ea834710959522c56406c9ad2022-12-22T01:56:41ZengBMCJournal of Medical Case Reports1752-19472019-09-011311610.1186/s13256-019-2222-5Acute human bocavirus 1 infection in child with life-threatening bilateral bronchiolitis and right-sided pneumonia: a case reportInga Ziemele0Man Xu1Anda Vilmane2Santa Rasa-Dzelzkaleja3Lea Hedman4Klaus Hedman5Maria Söderlund-Venermo6Zaiga Nora-Krukle7Modra Murovska8Dace Gardovska9Children’s Clinical University HospitalDepartment of Virology, University of HelsinkiInstitute of Microbiology and Virology, Rīga Stradiņš University RigaInstitute of Microbiology and Virology, Rīga Stradiņš University RigaDepartment of Virology, University of HelsinkiDepartment of Virology, University of HelsinkiDepartment of Virology, University of HelsinkiInstitute of Microbiology and Virology, Rīga Stradiņš University RigaInstitute of Microbiology and Virology, Rīga Stradiņš University RigaChildren’s Clinical University HospitalAbstract Background Human bocavirus 1 is a commonly detected human parvovirus. Many studies have shown human bocavirus 1 as a pathogen in association with acute respiratory tract infections in children. However, because human bocavirus 1 persists in the upper airways for extensive time periods after acute infection, the definition and diagnostics of acute human bocavirus 1 infection is challenging. Until now, detection of human bocavirus 1 exclusively, high viral load in respiratory samples, and viremia have been associated with a clinical picture of acute respiratory illness. There are no studies showing detection of human bocavirus 1 messenger ribonucleic acid in the peripheral blood mononuclear cells as a diagnostic marker for acute lower respiratory tract infection. Case presentation We report the case of a 17-month-old Latvian boy who presented in intensive care unit with acute bilateral bronchiolitis, with a history of rhinorrhea and cough for 6 days and fever for the last 2 days prior to admission, followed by severe respiratory distress and tracheal intubation. Human bocavirus 1 was the only respiratory virus detected by a qualitative multiplex polymerase chain reaction panel. For the diagnosis of acute human bocavirus 1 infection, both molecular and serological approaches were used. Human bocavirus 1 deoxyribonucleic acid (DNA) was detected simultaneously in nasopharyngeal aspirate, stool, and blood, as well as in the corresponding cell-free blood plasma by qualitative and quantitative polymerase chain reaction, revealing high DNA-copy numbers in nasopharyngeal aspirate and stool. Despite a low-load viremia, human bocavirus 1 messenger ribonucleic acid was found in the peripheral blood mononuclear cells. For detection of human bocavirus 1-specific antibodies, non-competitive immunoglobulin M and competitive immunoglobulin G enzyme immunoassays were used. The plasma was positive for both human bocavirus 1-specific immunoglobulin M and immunoglobulin G antibodies. Conclusions The presence of human bocavirus 1 genomic DNA in blood plasma and human bocavirus 1 messenger ribonucleic acid in peripheral blood mononuclear cells together with human bocavirus 1-specific immunoglobulin M are markers of acute human bocavirus 1 infection that may cause life-threatening acute bronchiolitis.http://link.springer.com/article/10.1186/s13256-019-2222-5Human bocavirus 1Lower respiratory tract infectionAcute bronchiolitisChildren |
| spellingShingle | Inga Ziemele Man Xu Anda Vilmane Santa Rasa-Dzelzkaleja Lea Hedman Klaus Hedman Maria Söderlund-Venermo Zaiga Nora-Krukle Modra Murovska Dace Gardovska Acute human bocavirus 1 infection in child with life-threatening bilateral bronchiolitis and right-sided pneumonia: a case report Journal of Medical Case Reports Human bocavirus 1 Lower respiratory tract infection Acute bronchiolitis Children |
| title | Acute human bocavirus 1 infection in child with life-threatening bilateral bronchiolitis and right-sided pneumonia: a case report |
| title_full | Acute human bocavirus 1 infection in child with life-threatening bilateral bronchiolitis and right-sided pneumonia: a case report |
| title_fullStr | Acute human bocavirus 1 infection in child with life-threatening bilateral bronchiolitis and right-sided pneumonia: a case report |
| title_full_unstemmed | Acute human bocavirus 1 infection in child with life-threatening bilateral bronchiolitis and right-sided pneumonia: a case report |
| title_short | Acute human bocavirus 1 infection in child with life-threatening bilateral bronchiolitis and right-sided pneumonia: a case report |
| title_sort | acute human bocavirus 1 infection in child with life threatening bilateral bronchiolitis and right sided pneumonia a case report |
| topic | Human bocavirus 1 Lower respiratory tract infection Acute bronchiolitis Children |
| url | http://link.springer.com/article/10.1186/s13256-019-2222-5 |
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