Typing of the Shiga toxin – Producing Escherichia coli strains isolated from diarrheic children using Multi Locus Sequence Typing (MLST)
Background and Aim: Shiga toxin-producing Escherichia coli (STEC) may also be referred to as Escherichia coli pathotypes, since E. coli serogroups O26 and O157 are frequently implicated in outbreaks of diarrhea in children. This study aimed to analyze the clone relationship of Shiga toxin-producing...
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| Format: | Article |
| Language: | English |
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Farname
2015-04-01
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| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://www.ijmm.ir/browse.php?a_code=A-10-338-1&slc_lang=en&sid=1 |
| _version_ | 1818665610425401344 |
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| author | Homa Khalafian Hassan Momtaz |
| author_facet | Homa Khalafian Hassan Momtaz |
| author_sort | Homa Khalafian |
| collection | DOAJ |
| description | Background and Aim: Shiga toxin-producing Escherichia coli (STEC) may also be referred to as Escherichia coli pathotypes, since E. coli serogroups O26 and O157 are frequently implicated in outbreaks of diarrhea in children. This study aimed to analyze the clone relationship of Shiga toxin-producing E. coli serogroups O157 and O26 isolated from diarrhea in children less than 60 months by Multilocus Sequence Typing (MLST) method.
Materials and Methods: A total of 20 Shiga toxins-producing E. coli (STEC strains were selected from two serogroups O26 and O157 isolated from diarrheic children, and the PCR products from amplification of seven housekeeping genes were sequenced in them. The nucleotide sequences of each gene in each isolate were analyzed, then queried to the MLST database and, in addition, determination of allele-specific of each gene were determined of sequence types (ST).
Results: A total of 3 clones ST10, ST11 and ST21 were identified from 20 isolates, which identified three gene clusters A, B and C, respectively.
Conclusions: Despite of the different ST were found in our isolates studied. There was no a high genetic diversity among the two serogroup O26 and O157 isolates, which indicates the clone relationship between E. coli isolates circulating in Tehran. |
| first_indexed | 2024-12-17T05:51:22Z |
| format | Article |
| id | doaj.art-773ff2b2b2f34709a5c20cfa0667a221 |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-12-17T05:51:22Z |
| publishDate | 2015-04-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-773ff2b2b2f34709a5c20cfa0667a2212022-12-21T22:01:08ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422015-04-01911421Typing of the Shiga toxin – Producing Escherichia coli strains isolated from diarrheic children using Multi Locus Sequence Typing (MLST)Homa Khalafian0Hassan Momtaz1 1. Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran. Background and Aim: Shiga toxin-producing Escherichia coli (STEC) may also be referred to as Escherichia coli pathotypes, since E. coli serogroups O26 and O157 are frequently implicated in outbreaks of diarrhea in children. This study aimed to analyze the clone relationship of Shiga toxin-producing E. coli serogroups O157 and O26 isolated from diarrhea in children less than 60 months by Multilocus Sequence Typing (MLST) method. Materials and Methods: A total of 20 Shiga toxins-producing E. coli (STEC strains were selected from two serogroups O26 and O157 isolated from diarrheic children, and the PCR products from amplification of seven housekeeping genes were sequenced in them. The nucleotide sequences of each gene in each isolate were analyzed, then queried to the MLST database and, in addition, determination of allele-specific of each gene were determined of sequence types (ST). Results: A total of 3 clones ST10, ST11 and ST21 were identified from 20 isolates, which identified three gene clusters A, B and C, respectively. Conclusions: Despite of the different ST were found in our isolates studied. There was no a high genetic diversity among the two serogroup O26 and O157 isolates, which indicates the clone relationship between E. coli isolates circulating in Tehran.http://www.ijmm.ir/browse.php?a_code=A-10-338-1&slc_lang=en&sid=1Shiga toxin Escherichia coli (STEC) Diarrheic children Multi-locus Sequence Typing (MLST) |
| spellingShingle | Homa Khalafian Hassan Momtaz Typing of the Shiga toxin – Producing Escherichia coli strains isolated from diarrheic children using Multi Locus Sequence Typing (MLST) Iranian Journal of Medical Microbiology Shiga toxin Escherichia coli (STEC) Diarrheic children Multi-locus Sequence Typing (MLST) |
| title | Typing of the Shiga toxin – Producing Escherichia coli strains isolated from diarrheic children using Multi Locus Sequence Typing (MLST) |
| title_full | Typing of the Shiga toxin – Producing Escherichia coli strains isolated from diarrheic children using Multi Locus Sequence Typing (MLST) |
| title_fullStr | Typing of the Shiga toxin – Producing Escherichia coli strains isolated from diarrheic children using Multi Locus Sequence Typing (MLST) |
| title_full_unstemmed | Typing of the Shiga toxin – Producing Escherichia coli strains isolated from diarrheic children using Multi Locus Sequence Typing (MLST) |
| title_short | Typing of the Shiga toxin – Producing Escherichia coli strains isolated from diarrheic children using Multi Locus Sequence Typing (MLST) |
| title_sort | typing of the shiga toxin producing escherichia coli strains isolated from diarrheic children using multi locus sequence typing mlst |
| topic | Shiga toxin Escherichia coli (STEC) Diarrheic children Multi-locus Sequence Typing (MLST) |
| url | http://www.ijmm.ir/browse.php?a_code=A-10-338-1&slc_lang=en&sid=1 |
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