Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion
Abstract Patients with tuberculous pleural effusion (TPE) or malignant pleural effusions (MPE) frequently have similar pleural fluid profiles. New biomarkers for the differential diagnosis of TPE are required. We determined whether cytokine profiles in the PE of patients could aid the differential d...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-79685-1 |
| _version_ | 1818749165758316544 |
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| author | Neda Dalil Roofchayee Majid Marjani Neda K. Dezfuli Payam Tabarsi Afshin Moniri Mohammad Varahram Ian M. Adcock Esmaeil Mortaz |
| author_facet | Neda Dalil Roofchayee Majid Marjani Neda K. Dezfuli Payam Tabarsi Afshin Moniri Mohammad Varahram Ian M. Adcock Esmaeil Mortaz |
| author_sort | Neda Dalil Roofchayee |
| collection | DOAJ |
| description | Abstract Patients with tuberculous pleural effusion (TPE) or malignant pleural effusions (MPE) frequently have similar pleural fluid profiles. New biomarkers for the differential diagnosis of TPE are required. We determined whether cytokine profiles in the PE of patients could aid the differential diagnosis of TPE. 30 patients with TPE, 30 patients with MPE, 14 patients with empyema (EMP) and 14 patients with parapneumonic effusion (PPE) were enrolled between Dec 2018 and 2019. The levels of interleukin (IL)-6, IL-18, IL-27, CXCL8, CCL-1 and IP-10 were determined in PE by ELISA along with measurements of adenosine deaminase (ADA). The best predictors of TPE were combined ADA.IL-27 [optimal cut-off value = 42.68 (103 U ng/l2), sensitivity 100%, specificity 98.28%], ADA [cut off value 27.5 (IU/l), sensitivity 90%, specificity 96.5%] and IL-27 [cut-off value = 2363 (pg/ml), sensitivity 96.7%, specificity 98.3%, p ≤ 0.0001]. A high level of IL-6 [cut-off value = 3260 (pg/ml), sensitivity 100%, specificity 67.2%], CXCL8 [cut-off value = 144.5 (pg/ml), sensitivity 93.3%, specificity 58.6%], CCL1 [cut-off value = 54 (pg/ml), sensitivity 100%, specificity 70.7%] and IP-10 [cut-off value = 891.9 (pg/ml), sensitivity 83.3%, specificity 48.3%] were also predictive of TPE. High ADA.IL-27, ADA and IL-27 levels differentiate between TPE and non-TPE with improved specificity and diagnostic accuracy and may be useful clinically. |
| first_indexed | 2024-12-18T03:59:27Z |
| format | Article |
| id | doaj.art-77415ce3851d45fca430221030ea6be0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T03:59:27Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-77415ce3851d45fca430221030ea6be02022-12-21T21:21:43ZengNature PortfolioScientific Reports2045-23222021-01-011111810.1038/s41598-020-79685-1Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusionNeda Dalil Roofchayee0Majid Marjani1Neda K. Dezfuli2Payam Tabarsi3Afshin Moniri4Mohammad Varahram5Ian M. Adcock6Esmaeil Mortaz7Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical SciencesClinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical SciencesDepartment of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical SciencesClinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical SciencesClinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical SciencesMycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical SciencesNational Heart and Lung Institute, Imperial CollegeDepartment of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical SciencesAbstract Patients with tuberculous pleural effusion (TPE) or malignant pleural effusions (MPE) frequently have similar pleural fluid profiles. New biomarkers for the differential diagnosis of TPE are required. We determined whether cytokine profiles in the PE of patients could aid the differential diagnosis of TPE. 30 patients with TPE, 30 patients with MPE, 14 patients with empyema (EMP) and 14 patients with parapneumonic effusion (PPE) were enrolled between Dec 2018 and 2019. The levels of interleukin (IL)-6, IL-18, IL-27, CXCL8, CCL-1 and IP-10 were determined in PE by ELISA along with measurements of adenosine deaminase (ADA). The best predictors of TPE were combined ADA.IL-27 [optimal cut-off value = 42.68 (103 U ng/l2), sensitivity 100%, specificity 98.28%], ADA [cut off value 27.5 (IU/l), sensitivity 90%, specificity 96.5%] and IL-27 [cut-off value = 2363 (pg/ml), sensitivity 96.7%, specificity 98.3%, p ≤ 0.0001]. A high level of IL-6 [cut-off value = 3260 (pg/ml), sensitivity 100%, specificity 67.2%], CXCL8 [cut-off value = 144.5 (pg/ml), sensitivity 93.3%, specificity 58.6%], CCL1 [cut-off value = 54 (pg/ml), sensitivity 100%, specificity 70.7%] and IP-10 [cut-off value = 891.9 (pg/ml), sensitivity 83.3%, specificity 48.3%] were also predictive of TPE. High ADA.IL-27, ADA and IL-27 levels differentiate between TPE and non-TPE with improved specificity and diagnostic accuracy and may be useful clinically.https://doi.org/10.1038/s41598-020-79685-1 |
| spellingShingle | Neda Dalil Roofchayee Majid Marjani Neda K. Dezfuli Payam Tabarsi Afshin Moniri Mohammad Varahram Ian M. Adcock Esmaeil Mortaz Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion Scientific Reports |
| title | Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion |
| title_full | Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion |
| title_fullStr | Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion |
| title_full_unstemmed | Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion |
| title_short | Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion |
| title_sort | potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion |
| url | https://doi.org/10.1038/s41598-020-79685-1 |
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