Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae)
Summary: Exchanges of mRNA were shown between host and stem parasites but not root parasites. Cistanche deserticola (Orobanchaceae) is a holoparasitic herb which parasitizes on the roots of woody plant Haloxylon ammodendron (Chenopodiaceae). We used transcriptome sequencing and bioinformatic analyse...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222021538 |
| _version_ | 1797946166100361216 |
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| author | Yanyan Fan Qiqi Zhao Huimin Duan Shuxin Bi Xiaomin Hao Rui Xu Runyao Bai Ruonan Yu Wenting Lu Tiejun Bao Hada Wuriyanghan |
| author_facet | Yanyan Fan Qiqi Zhao Huimin Duan Shuxin Bi Xiaomin Hao Rui Xu Runyao Bai Ruonan Yu Wenting Lu Tiejun Bao Hada Wuriyanghan |
| author_sort | Yanyan Fan |
| collection | DOAJ |
| description | Summary: Exchanges of mRNA were shown between host and stem parasites but not root parasites. Cistanche deserticola (Orobanchaceae) is a holoparasitic herb which parasitizes on the roots of woody plant Haloxylon ammodendron (Chenopodiaceae). We used transcriptome sequencing and bioinformatic analyses to identify nearly ten thousand mobile mRNAs. Transcript abundance appears to be a driving force for transfer event and mRNA exchanges occur through haustorial junction. Mobility of selected mRNAs was confirmed in situ and in sunflower-Orobanche cumana heterologous parasitic system. Four C. deserticola →H. ammodendron mobile mRNAs appear to facilitate haustorium development. Of interest, two mobile mRNAs of putative resistance genes CdNLR1 and CdNLR2 cause root-specific hypersensitive response and retard parasite development, which might contribute to parasitic equilibrium. The present study provides evidence for the large-scale mRNA transfer event between a woody host and a root parasite, and demonstrates the functional relevance of six C. deserticola genes in host-parasite interactions. |
| first_indexed | 2024-04-10T21:06:35Z |
| format | Article |
| id | doaj.art-77424c1042374c1da7cda2f0fe3e0897 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-10T21:06:35Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-77424c1042374c1da7cda2f0fe3e08972023-01-22T04:41:38ZengElsevieriScience2589-00422023-01-01261105880Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae)Yanyan Fan0Qiqi Zhao1Huimin Duan2Shuxin Bi3Xiaomin Hao4Rui Xu5Runyao Bai6Ruonan Yu7Wenting Lu8Tiejun Bao9Hada Wuriyanghan10Key Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, ChinaKey Laboratory of Ecology and Resource Use of the Mongolian Plateau, Ministry of Education, School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China; Corresponding authorKey Laboratory of Forage and Endemic Crop Biology, Ministry of Education, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China; Corresponding authorSummary: Exchanges of mRNA were shown between host and stem parasites but not root parasites. Cistanche deserticola (Orobanchaceae) is a holoparasitic herb which parasitizes on the roots of woody plant Haloxylon ammodendron (Chenopodiaceae). We used transcriptome sequencing and bioinformatic analyses to identify nearly ten thousand mobile mRNAs. Transcript abundance appears to be a driving force for transfer event and mRNA exchanges occur through haustorial junction. Mobility of selected mRNAs was confirmed in situ and in sunflower-Orobanche cumana heterologous parasitic system. Four C. deserticola →H. ammodendron mobile mRNAs appear to facilitate haustorium development. Of interest, two mobile mRNAs of putative resistance genes CdNLR1 and CdNLR2 cause root-specific hypersensitive response and retard parasite development, which might contribute to parasitic equilibrium. The present study provides evidence for the large-scale mRNA transfer event between a woody host and a root parasite, and demonstrates the functional relevance of six C. deserticola genes in host-parasite interactions.http://www.sciencedirect.com/science/article/pii/S2589004222021538Plant biologyInteraction of plants with organismsOmicsTranscriptomics |
| spellingShingle | Yanyan Fan Qiqi Zhao Huimin Duan Shuxin Bi Xiaomin Hao Rui Xu Runyao Bai Ruonan Yu Wenting Lu Tiejun Bao Hada Wuriyanghan Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae) iScience Plant biology Interaction of plants with organisms Omics Transcriptomics |
| title | Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae) |
| title_full | Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae) |
| title_fullStr | Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae) |
| title_full_unstemmed | Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae) |
| title_short | Large-scale mRNA transfer between Haloxylon ammodendron (Chenopodiaceae) and herbaceous root holoparasite Cistanche deserticola (Orobanchaceae) |
| title_sort | large scale mrna transfer between haloxylon ammodendron chenopodiaceae and herbaceous root holoparasite cistanche deserticola orobanchaceae |
| topic | Plant biology Interaction of plants with organisms Omics Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222021538 |
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