Upregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon–bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells
Abstract Background Anterior cruciate ligament (ACL) injury could lead to functional impairment along with disabilities. ACL reconstruction often fails owing to the regeneration failure of tendon–bone interface. Herein, we aimed to investigate the effects of Runt related transcription factor 1 (RUNX...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-022-03152-y |
| _version_ | 1798037657411911680 |
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| author | Kai Kang Qian Geng Lukuan Cui Lijie Wu Lei Zhang Tong Li Qian Zhang Shijun Gao |
| author_facet | Kai Kang Qian Geng Lukuan Cui Lijie Wu Lei Zhang Tong Li Qian Zhang Shijun Gao |
| author_sort | Kai Kang |
| collection | DOAJ |
| description | Abstract Background Anterior cruciate ligament (ACL) injury could lead to functional impairment along with disabilities. ACL reconstruction often fails owing to the regeneration failure of tendon–bone interface. Herein, we aimed to investigate the effects of Runt related transcription factor 1 (RUNX1) on tendon–bone healing after ACL reconstruction using bone mesenchymal stem cells (BMSCs). Methods BMSCs were isolated from the marrow cavity of rat femur, followed by the modification of RUNX1 with lentiviral system. Then, an ACL reconstruction model of rats was established with autografts. Results Results of flow cytometry exhibited positive-antigen CD44 and CD90, as well as negative-antigen CD34 and CD45 of the BMSCs. Then, we found that RUNX1-upregulated BMSCs elevated the decreased biomechanical strength of the tendon grafts after ACL reconstruction. Moreover, based on the histological observation, upregulation of RUNX1 was linked with better recovery around the bone tunnel, a tighter tendon–bone interface, and more collagen fibers compared to the group of BMSCs infected with LV-NC. Next, RUNX1-upregulated BMSCs promoted osteogenesis after ACL reconstruction, as evidenced by the mitigation of severe loss and erosion of the cartilage and bone in the tibial and femur area, as well as the increased number of osteoblasts identified by the upregulation of alkaline phosphatase, osteocalcin, and osteopontin in the tendon–bone interface. Conclusion Elevated expression of RUNX1 contributed to tendon–bone healing after ACL reconstruction using BMSCs. |
| first_indexed | 2024-04-11T21:29:31Z |
| format | Article |
| id | doaj.art-77427b3be9094b03b7c4bec0604bbb2f |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T21:29:31Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-77427b3be9094b03b7c4bec0604bbb2f2022-12-22T04:02:14ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2022-05-011711910.1186/s13018-022-03152-yUpregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon–bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cellsKai Kang0Qian Geng1Lukuan Cui2Lijie Wu3Lei Zhang4Tong Li5Qian Zhang6Shijun Gao7The Second Department of Joint Surgery, Third Hospital of Hebei Medical UniversityThe Second Department of Joint Surgery, Third Hospital of Hebei Medical UniversityThe Second Department of Joint Surgery, Third Hospital of Hebei Medical UniversityThe Second Department of Joint Surgery, Third Hospital of Hebei Medical UniversityThe Second Department of Joint Surgery, Third Hospital of Hebei Medical UniversityThe Second Department of Joint Surgery, Third Hospital of Hebei Medical UniversityThe Second Department of Joint Surgery, Third Hospital of Hebei Medical UniversityThe Second Department of Joint Surgery, Third Hospital of Hebei Medical UniversityAbstract Background Anterior cruciate ligament (ACL) injury could lead to functional impairment along with disabilities. ACL reconstruction often fails owing to the regeneration failure of tendon–bone interface. Herein, we aimed to investigate the effects of Runt related transcription factor 1 (RUNX1) on tendon–bone healing after ACL reconstruction using bone mesenchymal stem cells (BMSCs). Methods BMSCs were isolated from the marrow cavity of rat femur, followed by the modification of RUNX1 with lentiviral system. Then, an ACL reconstruction model of rats was established with autografts. Results Results of flow cytometry exhibited positive-antigen CD44 and CD90, as well as negative-antigen CD34 and CD45 of the BMSCs. Then, we found that RUNX1-upregulated BMSCs elevated the decreased biomechanical strength of the tendon grafts after ACL reconstruction. Moreover, based on the histological observation, upregulation of RUNX1 was linked with better recovery around the bone tunnel, a tighter tendon–bone interface, and more collagen fibers compared to the group of BMSCs infected with LV-NC. Next, RUNX1-upregulated BMSCs promoted osteogenesis after ACL reconstruction, as evidenced by the mitigation of severe loss and erosion of the cartilage and bone in the tibial and femur area, as well as the increased number of osteoblasts identified by the upregulation of alkaline phosphatase, osteocalcin, and osteopontin in the tendon–bone interface. Conclusion Elevated expression of RUNX1 contributed to tendon–bone healing after ACL reconstruction using BMSCs.https://doi.org/10.1186/s13018-022-03152-yRunt related transcription factor 1Anterior cruciate ligament reconstructionTendon–bone healingBone mesenchymal stem cells |
| spellingShingle | Kai Kang Qian Geng Lukuan Cui Lijie Wu Lei Zhang Tong Li Qian Zhang Shijun Gao Upregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon–bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells Journal of Orthopaedic Surgery and Research Runt related transcription factor 1 Anterior cruciate ligament reconstruction Tendon–bone healing Bone mesenchymal stem cells |
| title | Upregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon–bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells |
| title_full | Upregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon–bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells |
| title_fullStr | Upregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon–bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells |
| title_full_unstemmed | Upregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon–bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells |
| title_short | Upregulation of Runt related transcription factor 1 (RUNX1) contributes to tendon–bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells |
| title_sort | upregulation of runt related transcription factor 1 runx1 contributes to tendon bone healing after anterior cruciate ligament reconstruction using bone mesenchymal stem cells |
| topic | Runt related transcription factor 1 Anterior cruciate ligament reconstruction Tendon–bone healing Bone mesenchymal stem cells |
| url | https://doi.org/10.1186/s13018-022-03152-y |
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