Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
Collagen-rich tissues have poor reparative capacity that predisposes to common age-related disorders such as osteoporosis and osteoarthritis. We used in vivo pulsed SILAC labelling to quantify new protein incorporation into cartilage, bone, and skin of mice across the healthy life course. We report...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2021-09-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/66635 |
| _version_ | 1811227737312985088 |
|---|---|
| author | Yoanna Ariosa-Morejon Alberto Santos Roman Fischer Simon Davis Philip Charles Rajesh Thakker Angus KT Wann Tonia L Vincent |
| author_facet | Yoanna Ariosa-Morejon Alberto Santos Roman Fischer Simon Davis Philip Charles Rajesh Thakker Angus KT Wann Tonia L Vincent |
| author_sort | Yoanna Ariosa-Morejon |
| collection | DOAJ |
| description | Collagen-rich tissues have poor reparative capacity that predisposes to common age-related disorders such as osteoporosis and osteoarthritis. We used in vivo pulsed SILAC labelling to quantify new protein incorporation into cartilage, bone, and skin of mice across the healthy life course. We report dynamic turnover of the matrisome, the proteins of the extracellular matrix, in bone and cartilage during skeletal maturation, which was markedly reduced after skeletal maturity. Comparing young adult with older adult mice, new protein incorporation was reduced in all tissues. STRING clustering revealed changes in epigenetic modulators across all tissues, a decline in chondroprotective growth factors such as FGF2 and TGFβ in cartilage, and clusters indicating mitochondrial dysregulation and reduced collagen synthesis in bone. Several pathways were implicated in age-related disease. Fewer changes were observed for skin. This methodology provides dynamic protein data at a tissue level, uncovering age-related molecular changes that may predispose to disease. |
| first_indexed | 2024-04-12T09:46:41Z |
| format | Article |
| id | doaj.art-7742f5d2092644ec824ba5a576a98084 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:46:41Z |
| publishDate | 2021-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-7742f5d2092644ec824ba5a576a980842022-12-22T03:37:56ZengeLife Sciences Publications LtdeLife2050-084X2021-09-011010.7554/eLife.66635Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labellingYoanna Ariosa-Morejon0Alberto Santos1Roman Fischer2https://orcid.org/0000-0002-9715-5951Simon Davis3Philip Charles4https://orcid.org/0000-0001-5278-5354Rajesh Thakker5https://orcid.org/0000-0002-1438-3220Angus KT Wann6Tonia L Vincent7https://orcid.org/0000-0002-3412-5712Kennedy Institute of Rheumatology, Arthritis Research UK Centre for OA Pathogenesis, University of Oxford, Oxford, United KingdomBig Data Institute, Li-Ka Shing Centre for Health Information and Discovery, University of Oxford, Oxford, United Kingdom; Center for Health Data Science, Faculty of Health Sciences, University of Copenhagen, Copenhagen, United KingdomNuffield Department of Medicine, Target Discovery Institute, University of Oxford, Oxford, United KingdomNuffield Department of Medicine, Target Discovery Institute, University of Oxford, Oxford, United KingdomNuffield Department of Medicine, Target Discovery Institute, University of Oxford, Oxford, United KingdomAcademic Endocrine Unit, OCDEM, Churchill Hospital, University of Oxford, Oxford, United KingdomKennedy Institute of Rheumatology, Arthritis Research UK Centre for OA Pathogenesis, University of Oxford, Oxford, United KingdomKennedy Institute of Rheumatology, Arthritis Research UK Centre for OA Pathogenesis, University of Oxford, Oxford, United KingdomCollagen-rich tissues have poor reparative capacity that predisposes to common age-related disorders such as osteoporosis and osteoarthritis. We used in vivo pulsed SILAC labelling to quantify new protein incorporation into cartilage, bone, and skin of mice across the healthy life course. We report dynamic turnover of the matrisome, the proteins of the extracellular matrix, in bone and cartilage during skeletal maturation, which was markedly reduced after skeletal maturity. Comparing young adult with older adult mice, new protein incorporation was reduced in all tissues. STRING clustering revealed changes in epigenetic modulators across all tissues, a decline in chondroprotective growth factors such as FGF2 and TGFβ in cartilage, and clusters indicating mitochondrial dysregulation and reduced collagen synthesis in bone. Several pathways were implicated in age-related disease. Fewer changes were observed for skin. This methodology provides dynamic protein data at a tissue level, uncovering age-related molecular changes that may predispose to disease.https://elifesciences.org/articles/66635Ageprotein dynamicsproteomicsSILACmatrisomecollagen-rich tissues |
| spellingShingle | Yoanna Ariosa-Morejon Alberto Santos Roman Fischer Simon Davis Philip Charles Rajesh Thakker Angus KT Wann Tonia L Vincent Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling eLife Age protein dynamics proteomics SILAC matrisome collagen-rich tissues |
| title | Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling |
| title_full | Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling |
| title_fullStr | Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling |
| title_full_unstemmed | Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling |
| title_short | Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling |
| title_sort | age dependent changes in protein incorporation into collagen rich tissues of mice by in vivo pulsed silac labelling |
| topic | Age protein dynamics proteomics SILAC matrisome collagen-rich tissues |
| url | https://elifesciences.org/articles/66635 |
| work_keys_str_mv | AT yoannaariosamorejon agedependentchangesinproteinincorporationintocollagenrichtissuesofmicebyinvivopulsedsilaclabelling AT albertosantos agedependentchangesinproteinincorporationintocollagenrichtissuesofmicebyinvivopulsedsilaclabelling AT romanfischer agedependentchangesinproteinincorporationintocollagenrichtissuesofmicebyinvivopulsedsilaclabelling AT simondavis agedependentchangesinproteinincorporationintocollagenrichtissuesofmicebyinvivopulsedsilaclabelling AT philipcharles agedependentchangesinproteinincorporationintocollagenrichtissuesofmicebyinvivopulsedsilaclabelling AT rajeshthakker agedependentchangesinproteinincorporationintocollagenrichtissuesofmicebyinvivopulsedsilaclabelling AT angusktwann agedependentchangesinproteinincorporationintocollagenrichtissuesofmicebyinvivopulsedsilaclabelling AT tonialvincent agedependentchangesinproteinincorporationintocollagenrichtissuesofmicebyinvivopulsedsilaclabelling |