In Vitro Cell Culture of <i>Rhus coriaria</i> L.: A Standardized Phytocomplex Rich of Gallic Acid Derivatives with Antioxidant and Skin Repair Activity
This study focused on the biological evaluation and chemical characterization of a new ingredient obtained by in vitro cell culture of <i>Rhus coriaria</i> L. An in vitro plant cell culture method permits to cultivate plant in a short period of time and to obtain extract with a high safe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Cosmetics |
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| Online Access: | https://www.mdpi.com/2079-9284/9/1/12 |
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| author | Giovanna Pressi Oriana Bertaiola Chiara Guarnerio Elisa Barbieri Giovanna Rigillo Paolo Governa Marco Biagi Flavia Guzzo Alessandra Semenzato |
| author_facet | Giovanna Pressi Oriana Bertaiola Chiara Guarnerio Elisa Barbieri Giovanna Rigillo Paolo Governa Marco Biagi Flavia Guzzo Alessandra Semenzato |
| author_sort | Giovanna Pressi |
| collection | DOAJ |
| description | This study focused on the biological evaluation and chemical characterization of a new ingredient obtained by in vitro cell culture of <i>Rhus coriaria</i> L. An in vitro plant cell culture method permits to cultivate plant in a short period of time and to obtain extract with a high safety profile for the consumer, free from heavy metals, pesticides, aflatoxins, bacterial or fungal contamination. Through the selection of specific cell culture media, it was possible to obtain a <i>Rhus coriaria</i> cell line with a high content of gallic acid derivatives. The <i>Rhus coriaria</i> L. phytocomplex (RC-P), containing 7.6% <i>w</i>/<i>w</i> of acid gallic derivatives, was obtained by drying of plant cell biomass after 14 days of growth in the final selected culture medium. UPLC-ESI-MS and UPLC-DAD analysis allowed to identify numerous gallic acid derivatives, such as galloyl hexose, trigalloyl hexose and high molecular weight galloyl derivatives, and to quantify their overall content. The antioxidant activity of the RC-P was tested by DPPH assay and the wound healing activity was evaluated using a scratch wound healing test on human keratinocytes and fibroblasts. This work showed that RC-P could be a new effective cosmetic ingredient with antioxidant and skin repair activity. |
| first_indexed | 2024-03-09T22:16:19Z |
| format | Article |
| id | doaj.art-77430398885e48f0a4cd1a6aa13fafad |
| institution | Directory Open Access Journal |
| issn | 2079-9284 |
| language | English |
| last_indexed | 2024-03-09T22:16:19Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cosmetics |
| spelling | doaj.art-77430398885e48f0a4cd1a6aa13fafad2023-11-23T19:23:03ZengMDPI AGCosmetics2079-92842022-01-01911210.3390/cosmetics9010012In Vitro Cell Culture of <i>Rhus coriaria</i> L.: A Standardized Phytocomplex Rich of Gallic Acid Derivatives with Antioxidant and Skin Repair ActivityGiovanna Pressi0Oriana Bertaiola1Chiara Guarnerio2Elisa Barbieri3Giovanna Rigillo4Paolo Governa5Marco Biagi6Flavia Guzzo7Alessandra Semenzato8Aethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyAethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyAethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyAethera Biotech s.r.l., 36043 Camisano Vicentino, ItalyDepartment of Life Sciences, University of Modena and Reggio Emilia, 41125 Modena, ItalyDepartment of Biotechnology, Chemistry and Pharmacy—Department of Excellence 2018-2022, University of Siena, 53100 Siena, ItalyDepartment of Physical Sciences, Earth and Environment, University of Siena, 53100 Siena, ItalyDepartment of Biotechnology, University of Verona, 37134 Verona, ItalyDepartment of Pharmaceutical and Pharmacological Sciences, University of Padua, 35131 Padua, ItalyThis study focused on the biological evaluation and chemical characterization of a new ingredient obtained by in vitro cell culture of <i>Rhus coriaria</i> L. An in vitro plant cell culture method permits to cultivate plant in a short period of time and to obtain extract with a high safety profile for the consumer, free from heavy metals, pesticides, aflatoxins, bacterial or fungal contamination. Through the selection of specific cell culture media, it was possible to obtain a <i>Rhus coriaria</i> cell line with a high content of gallic acid derivatives. The <i>Rhus coriaria</i> L. phytocomplex (RC-P), containing 7.6% <i>w</i>/<i>w</i> of acid gallic derivatives, was obtained by drying of plant cell biomass after 14 days of growth in the final selected culture medium. UPLC-ESI-MS and UPLC-DAD analysis allowed to identify numerous gallic acid derivatives, such as galloyl hexose, trigalloyl hexose and high molecular weight galloyl derivatives, and to quantify their overall content. The antioxidant activity of the RC-P was tested by DPPH assay and the wound healing activity was evaluated using a scratch wound healing test on human keratinocytes and fibroblasts. This work showed that RC-P could be a new effective cosmetic ingredient with antioxidant and skin repair activity.https://www.mdpi.com/2079-9284/9/1/12<i>Rhus coriaria</i>phytocomplexin vitro plant cell cultureantioxidantwound healing |
| spellingShingle | Giovanna Pressi Oriana Bertaiola Chiara Guarnerio Elisa Barbieri Giovanna Rigillo Paolo Governa Marco Biagi Flavia Guzzo Alessandra Semenzato In Vitro Cell Culture of <i>Rhus coriaria</i> L.: A Standardized Phytocomplex Rich of Gallic Acid Derivatives with Antioxidant and Skin Repair Activity Cosmetics <i>Rhus coriaria</i> phytocomplex in vitro plant cell culture antioxidant wound healing |
| title | In Vitro Cell Culture of <i>Rhus coriaria</i> L.: A Standardized Phytocomplex Rich of Gallic Acid Derivatives with Antioxidant and Skin Repair Activity |
| title_full | In Vitro Cell Culture of <i>Rhus coriaria</i> L.: A Standardized Phytocomplex Rich of Gallic Acid Derivatives with Antioxidant and Skin Repair Activity |
| title_fullStr | In Vitro Cell Culture of <i>Rhus coriaria</i> L.: A Standardized Phytocomplex Rich of Gallic Acid Derivatives with Antioxidant and Skin Repair Activity |
| title_full_unstemmed | In Vitro Cell Culture of <i>Rhus coriaria</i> L.: A Standardized Phytocomplex Rich of Gallic Acid Derivatives with Antioxidant and Skin Repair Activity |
| title_short | In Vitro Cell Culture of <i>Rhus coriaria</i> L.: A Standardized Phytocomplex Rich of Gallic Acid Derivatives with Antioxidant and Skin Repair Activity |
| title_sort | in vitro cell culture of i rhus coriaria i l a standardized phytocomplex rich of gallic acid derivatives with antioxidant and skin repair activity |
| topic | <i>Rhus coriaria</i> phytocomplex in vitro plant cell culture antioxidant wound healing |
| url | https://www.mdpi.com/2079-9284/9/1/12 |
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