Infection of a Lepidopteran Cell Line with Deformed Wing Virus
Many attempts to develop a reliable cell cultured-based system to study honey bee virus infections have encountered substantial difficulties. We investigated the ability of a cell line from a heterologous insect to sustain infection by a honey bee virus. For this purpose, we infected the Lepidoptera...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/7/739 |
| _version_ | 1797562934060122112 |
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| author | Tal Erez Nor Chejanovsky |
| author_facet | Tal Erez Nor Chejanovsky |
| author_sort | Tal Erez |
| collection | DOAJ |
| description | Many attempts to develop a reliable cell cultured-based system to study honey bee virus infections have encountered substantial difficulties. We investigated the ability of a cell line from a heterologous insect to sustain infection by a honey bee virus. For this purpose, we infected the Lepidopteran hemocytic cell line (P1) with Deformed wing virus (DWV). The genomic copies of DWV increased upon infection, as monitored by quantitative RT-PCR. Moreover, a tagged-primer-based RT-PCR analysis showed the presence of DWV negative-sense RNA in the cells, indicating virus replication. However, the DWV from infected cells was mildly infectious to P1 cells. Similar results were obtained when the virus was injected into <i>Apis mellifera</i> pupae. Thus, though the virus yields from the infected cells appeared to be very low, we show for the first time that DWV can replicate in a heterologous cell line. Given the availability of many other insect cell lines, our study paves the way for future exploration in this direction. In the absence of adequate <i>A. mellifera</i> cell lines, exploring the ability of alternative cell lines to enable honey bee virus infections could provide the means to study and understand the viral infectious cycle at the cellular level and facilitate obtaining purified isolates of these viruses. |
| first_indexed | 2024-03-10T18:35:38Z |
| format | Article |
| id | doaj.art-77441072c48c4b19bd0b9547435095a1 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T18:35:38Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-77441072c48c4b19bd0b9547435095a12023-11-20T06:15:22ZengMDPI AGViruses1999-49152020-07-0112773910.3390/v12070739Infection of a Lepidopteran Cell Line with Deformed Wing VirusTal Erez0Nor Chejanovsky1Department of Entomology Institute of Plant Protection, Agricultural Research Organization, Rishon Lezion 7528809, IsraelDepartment of Entomology Institute of Plant Protection, Agricultural Research Organization, Rishon Lezion 7528809, IsraelMany attempts to develop a reliable cell cultured-based system to study honey bee virus infections have encountered substantial difficulties. We investigated the ability of a cell line from a heterologous insect to sustain infection by a honey bee virus. For this purpose, we infected the Lepidopteran hemocytic cell line (P1) with Deformed wing virus (DWV). The genomic copies of DWV increased upon infection, as monitored by quantitative RT-PCR. Moreover, a tagged-primer-based RT-PCR analysis showed the presence of DWV negative-sense RNA in the cells, indicating virus replication. However, the DWV from infected cells was mildly infectious to P1 cells. Similar results were obtained when the virus was injected into <i>Apis mellifera</i> pupae. Thus, though the virus yields from the infected cells appeared to be very low, we show for the first time that DWV can replicate in a heterologous cell line. Given the availability of many other insect cell lines, our study paves the way for future exploration in this direction. In the absence of adequate <i>A. mellifera</i> cell lines, exploring the ability of alternative cell lines to enable honey bee virus infections could provide the means to study and understand the viral infectious cycle at the cellular level and facilitate obtaining purified isolates of these viruses.https://www.mdpi.com/1999-4915/12/7/739Deformed wing virushoney bee virusLepidopteran cells |
| spellingShingle | Tal Erez Nor Chejanovsky Infection of a Lepidopteran Cell Line with Deformed Wing Virus Viruses Deformed wing virus honey bee virus Lepidopteran cells |
| title | Infection of a Lepidopteran Cell Line with Deformed Wing Virus |
| title_full | Infection of a Lepidopteran Cell Line with Deformed Wing Virus |
| title_fullStr | Infection of a Lepidopteran Cell Line with Deformed Wing Virus |
| title_full_unstemmed | Infection of a Lepidopteran Cell Line with Deformed Wing Virus |
| title_short | Infection of a Lepidopteran Cell Line with Deformed Wing Virus |
| title_sort | infection of a lepidopteran cell line with deformed wing virus |
| topic | Deformed wing virus honey bee virus Lepidopteran cells |
| url | https://www.mdpi.com/1999-4915/12/7/739 |
| work_keys_str_mv | AT talerez infectionofalepidopterancelllinewithdeformedwingvirus AT norchejanovsky infectionofalepidopterancelllinewithdeformedwingvirus |