Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate
The utility of a next generation biosynthetic bacterial 6-phytase (PhyG) in restoring bone ash, bone phosphorus (P) content and performance in piglets depleted in P was evaluated. A total of 9 treatments were tested as follows. Treatment 1, a negative control (NC) diet; treatments 2, 3, 4, NC supple...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2020-03-01
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| Series: | Animal Nutrition |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654519301866 |
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| author | Yueming Dersjant-Li Boris Villca Vincent Sewalt Arno de Kreij Leon Marchal Deepak E. Velayudhan Robin A. Sorg Trine Christensen Rie Mejldal Igor Nikolaev Sina Pricelius Hye-Sook Kim Svend Haaning Jens F. Sørensen Rosil Lizardo |
| author_facet | Yueming Dersjant-Li Boris Villca Vincent Sewalt Arno de Kreij Leon Marchal Deepak E. Velayudhan Robin A. Sorg Trine Christensen Rie Mejldal Igor Nikolaev Sina Pricelius Hye-Sook Kim Svend Haaning Jens F. Sørensen Rosil Lizardo |
| author_sort | Yueming Dersjant-Li |
| collection | DOAJ |
| description | The utility of a next generation biosynthetic bacterial 6-phytase (PhyG) in restoring bone ash, bone phosphorus (P) content and performance in piglets depleted in P was evaluated. A total of 9 treatments were tested as follows. Treatment 1, a negative control (NC) diet; treatments 2, 3, 4, NC supplemented with 250, 500 or 1,000 FTU/kg of PhyG; treatments 5, 6, NC supplemented with 500 or 1,000 FTU/kg of a commercial Buttiauxella sp phytase (PhyB); treatments 7, 8, 9, NC supplemented with monocalcium phosphate (MCP) to provide 0.7, 1.4 and 1.8 g/kg digestible P, equating to a digestible P content of 1.8, 2.5 and 2.9 g/kg. The latter constituting the positive control (PC) diet with adequate P and calcium (Ca). The NC was formulated without inorganic P (1.1 g digestible P/kg) and reduced in Ca (5.0 g/kg). Additional limestone was added to treatments 7 to 9 to maintain Ca-to-P ratio between 1.2 and 1.3. A total of 162 crossed Pietrain × (Large White × Landrace) 21-d-old piglets (50% males and 50% females) were fed adaptation diets until 42 d old and then assigned to pens with 2 pigs/pen and 9 pens/treatment in a completely randomized block design. Piglets were fed mash diets based on corn and soybean meal ad libitum for 28 d. At the end of the study, one piglet perpen was euthanized and the right feet collected for determination of bone strength, bone ash and mineral content. Compared with the PC, the NC group had reduced average daily gain (ADG) and increased feed conversion ratio (FCR) during all growth phases and overall, and at d 28 (70 d old) NC pigs had bones with reduced ash, Ca and P content (P < 0.05). The PhyG at 250 FTU/kg improved bone ash vs. NC. Increasing PhyG dose linearly or quadratically improved bone ash, ADG and FCR (P < 0.05). At ≥ 500 FTU/kg, both PhyG and PhyB maintained ADG and FCR equivalent to PC. Linear regression analysis was done to compare the measured response parameters to increasing digestible P from MCP. Based on this analysis it was shown that PhyG and PhyB at 1,000 FTU/kg could replace 1.83 and 1.66 g/kg digestible P from MCP in the diet, respectively, on average across metacarpi bone ash, ADG or FCR. These findings suggest that the biosynthetic phytase is highly effective in the tested dietary setting. Keywords: Biosynthetic bacterial 6-phytase, Bone ash, Efficacy, Growth performance, Pigs |
| first_indexed | 2024-12-17T05:24:34Z |
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| id | doaj.art-7745f0aba75945ccbac10669b63cd633 |
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| issn | 2405-6545 |
| language | English |
| last_indexed | 2024-12-17T05:24:34Z |
| publishDate | 2020-03-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Animal Nutrition |
| spelling | doaj.art-7745f0aba75945ccbac10669b63cd6332022-12-21T22:01:54ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452020-03-01612430Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphateYueming Dersjant-Li0Boris Villca1Vincent Sewalt2Arno de Kreij3Leon Marchal4Deepak E. Velayudhan5Robin A. Sorg6Trine Christensen7Rie Mejldal8Igor Nikolaev9Sina Pricelius10Hye-Sook Kim11Svend Haaning12Jens F. Sørensen13Rosil Lizardo14DuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the Netherlands; Corresponding author.Institut de Recerca I Tecnologia Agroalimentàries, Centre Mas de Bover, Ctra. Reus-El Morell km. 3.8, E-43120, Constantí, SpainDuPont Nutrition & Biosciences, 925 Page Mill Road, Palo Alto, CA 94304, USADuPont Nutrition & Biosciences, 21 Biopolis Road, Nucleos, South Tower, 138567, SingaporeDuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the NetherlandsDuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the NetherlandsDuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the NetherlandsDuPont Nutrition and Biosciences, Edwin Rahrs Vej 38, DK-8220, Brabrand, DenmarkDuPont Nutrition and Biosciences, Edwin Rahrs Vej 38, DK-8220, Brabrand, DenmarkDuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the NetherlandsDuPont Nutrition and Biosciences, Archimedesweg, 30, 2333, CN Leiden, the NetherlandsDuPont Nutrition & Biosciences, 925 Page Mill Road, Palo Alto, CA 94304, USADuPont Nutrition and Biosciences, Edwin Rahrs Vej 38, DK-8220, Brabrand, DenmarkDuPont Nutrition and Biosciences, Edwin Rahrs Vej 38, DK-8220, Brabrand, DenmarkInstitut de Recerca I Tecnologia Agroalimentàries, Centre Mas de Bover, Ctra. Reus-El Morell km. 3.8, E-43120, Constantí, SpainThe utility of a next generation biosynthetic bacterial 6-phytase (PhyG) in restoring bone ash, bone phosphorus (P) content and performance in piglets depleted in P was evaluated. A total of 9 treatments were tested as follows. Treatment 1, a negative control (NC) diet; treatments 2, 3, 4, NC supplemented with 250, 500 or 1,000 FTU/kg of PhyG; treatments 5, 6, NC supplemented with 500 or 1,000 FTU/kg of a commercial Buttiauxella sp phytase (PhyB); treatments 7, 8, 9, NC supplemented with monocalcium phosphate (MCP) to provide 0.7, 1.4 and 1.8 g/kg digestible P, equating to a digestible P content of 1.8, 2.5 and 2.9 g/kg. The latter constituting the positive control (PC) diet with adequate P and calcium (Ca). The NC was formulated without inorganic P (1.1 g digestible P/kg) and reduced in Ca (5.0 g/kg). Additional limestone was added to treatments 7 to 9 to maintain Ca-to-P ratio between 1.2 and 1.3. A total of 162 crossed Pietrain × (Large White × Landrace) 21-d-old piglets (50% males and 50% females) were fed adaptation diets until 42 d old and then assigned to pens with 2 pigs/pen and 9 pens/treatment in a completely randomized block design. Piglets were fed mash diets based on corn and soybean meal ad libitum for 28 d. At the end of the study, one piglet perpen was euthanized and the right feet collected for determination of bone strength, bone ash and mineral content. Compared with the PC, the NC group had reduced average daily gain (ADG) and increased feed conversion ratio (FCR) during all growth phases and overall, and at d 28 (70 d old) NC pigs had bones with reduced ash, Ca and P content (P < 0.05). The PhyG at 250 FTU/kg improved bone ash vs. NC. Increasing PhyG dose linearly or quadratically improved bone ash, ADG and FCR (P < 0.05). At ≥ 500 FTU/kg, both PhyG and PhyB maintained ADG and FCR equivalent to PC. Linear regression analysis was done to compare the measured response parameters to increasing digestible P from MCP. Based on this analysis it was shown that PhyG and PhyB at 1,000 FTU/kg could replace 1.83 and 1.66 g/kg digestible P from MCP in the diet, respectively, on average across metacarpi bone ash, ADG or FCR. These findings suggest that the biosynthetic phytase is highly effective in the tested dietary setting. Keywords: Biosynthetic bacterial 6-phytase, Bone ash, Efficacy, Growth performance, Pigshttp://www.sciencedirect.com/science/article/pii/S2405654519301866 |
| spellingShingle | Yueming Dersjant-Li Boris Villca Vincent Sewalt Arno de Kreij Leon Marchal Deepak E. Velayudhan Robin A. Sorg Trine Christensen Rie Mejldal Igor Nikolaev Sina Pricelius Hye-Sook Kim Svend Haaning Jens F. Sørensen Rosil Lizardo Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate Animal Nutrition |
| title | Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate |
| title_full | Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate |
| title_fullStr | Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate |
| title_full_unstemmed | Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate |
| title_short | Functionality of a next generation biosynthetic bacterial 6-phytase in enhancing phosphorus availability to weaned piglets fed a corn-soybean meal-based diet without added inorganic phosphate |
| title_sort | functionality of a next generation biosynthetic bacterial 6 phytase in enhancing phosphorus availability to weaned piglets fed a corn soybean meal based diet without added inorganic phosphate |
| url | http://www.sciencedirect.com/science/article/pii/S2405654519301866 |
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