Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice
The present study investigates the antioxidant and hepatoprotective effects of Capparis spinosa L. and Quercetin in tert-butyl hydroperoxide (t-BHP) induced acute liver damage. Different fractions of C. spinosa were examined for total phenolic content and antioxidant property. Among these fractions,...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-01-01
|
| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411017300500 |
| _version_ | 1828367774487937024 |
|---|---|
| author | Heibatullah Kalantari Hossein Foruozandeh Mohammad Javad Khodayar Amir Siahpoosh Najmaldin Saki Parvin Kheradmand |
| author_facet | Heibatullah Kalantari Hossein Foruozandeh Mohammad Javad Khodayar Amir Siahpoosh Najmaldin Saki Parvin Kheradmand |
| author_sort | Heibatullah Kalantari |
| collection | DOAJ |
| description | The present study investigates the antioxidant and hepatoprotective effects of Capparis spinosa L. and Quercetin in tert-butyl hydroperoxide (t-BHP) induced acute liver damage. Different fractions of C. spinosa were examined for total phenolic content and antioxidant property. Among these fractions, hydroalcoholic extract was used to assess the hepatoprotective effect in tert-butyl hydroperoxide (t-BHP) induced hepatotoxicity model by determining serum biochemical markers, sleeping time and antioxidant assay such as reduced glutathione (GSH) as well as histopathological examination of liver tissues. The total phenolic and Quercetin contents of hydroalcoholic fraction were significantly higher than other fractions. It also showed high antioxidant activity. Pretreatment with hydroalcoholic fraction at the dose of 400 mg/kg and Quercetin at the dose of 20 mg/kg showed liver protection against t-BHP induced hepatic injury, as it was evident by a significant decrease in serum enzymes marker, sleeping time and MDA and an increase in the GSH, SOD and CAT activities confirmed by pathology tests. The final results ascertained the hepatoprotective and antioxidant effects of C. spinosa and Quercetin in a dose-dependent manner. Moreover, this study suggests that possible mechanism of this protection may be associated with its property of scavenging free radicals which may be due to the presence of phenolic compounds. |
| first_indexed | 2024-04-14T06:02:36Z |
| format | Article |
| id | doaj.art-77464c4a4d8c4b9ca7f126f8888f678f |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-04-14T06:02:36Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-77464c4a4d8c4b9ca7f126f8888f678f2022-12-22T02:08:41ZengElsevierJournal of Traditional and Complementary Medicine2225-41102018-01-018112012710.1016/j.jtcme.2017.04.010Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in miceHeibatullah Kalantari0Hossein Foruozandeh1Mohammad Javad Khodayar2Amir Siahpoosh3Najmaldin Saki4Parvin Kheradmand5Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranMedicinal Plants Research Center, Department of Pharmacognosy, School of Pharmacy, Ahvaz Jundishapur University of Medical Science, Ahvaz, IranHealth Research Institute, Research Center of Thalassemia & Hemoglobinopathy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Pathology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranThe present study investigates the antioxidant and hepatoprotective effects of Capparis spinosa L. and Quercetin in tert-butyl hydroperoxide (t-BHP) induced acute liver damage. Different fractions of C. spinosa were examined for total phenolic content and antioxidant property. Among these fractions, hydroalcoholic extract was used to assess the hepatoprotective effect in tert-butyl hydroperoxide (t-BHP) induced hepatotoxicity model by determining serum biochemical markers, sleeping time and antioxidant assay such as reduced glutathione (GSH) as well as histopathological examination of liver tissues. The total phenolic and Quercetin contents of hydroalcoholic fraction were significantly higher than other fractions. It also showed high antioxidant activity. Pretreatment with hydroalcoholic fraction at the dose of 400 mg/kg and Quercetin at the dose of 20 mg/kg showed liver protection against t-BHP induced hepatic injury, as it was evident by a significant decrease in serum enzymes marker, sleeping time and MDA and an increase in the GSH, SOD and CAT activities confirmed by pathology tests. The final results ascertained the hepatoprotective and antioxidant effects of C. spinosa and Quercetin in a dose-dependent manner. Moreover, this study suggests that possible mechanism of this protection may be associated with its property of scavenging free radicals which may be due to the presence of phenolic compounds.http://www.sciencedirect.com/science/article/pii/S2225411017300500Capparis spinosat-butyl hydroperoxideQuercetinAntioxidantHepatoprotective |
| spellingShingle | Heibatullah Kalantari Hossein Foruozandeh Mohammad Javad Khodayar Amir Siahpoosh Najmaldin Saki Parvin Kheradmand Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice Journal of Traditional and Complementary Medicine Capparis spinosa t-butyl hydroperoxide Quercetin Antioxidant Hepatoprotective |
| title | Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice |
| title_full | Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice |
| title_fullStr | Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice |
| title_full_unstemmed | Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice |
| title_short | Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice |
| title_sort | antioxidant and hepatoprotective effects of capparis spinosa l fractions and quercetin on tert butyl hydroperoxide induced acute liver damage in mice |
| topic | Capparis spinosa t-butyl hydroperoxide Quercetin Antioxidant Hepatoprotective |
| url | http://www.sciencedirect.com/science/article/pii/S2225411017300500 |
| work_keys_str_mv | AT heibatullahkalantari antioxidantandhepatoprotectiveeffectsofcapparisspinosalfractionsandquercetinontertbutylhydroperoxideinducedacuteliverdamageinmice AT hosseinforuozandeh antioxidantandhepatoprotectiveeffectsofcapparisspinosalfractionsandquercetinontertbutylhydroperoxideinducedacuteliverdamageinmice AT mohammadjavadkhodayar antioxidantandhepatoprotectiveeffectsofcapparisspinosalfractionsandquercetinontertbutylhydroperoxideinducedacuteliverdamageinmice AT amirsiahpoosh antioxidantandhepatoprotectiveeffectsofcapparisspinosalfractionsandquercetinontertbutylhydroperoxideinducedacuteliverdamageinmice AT najmaldinsaki antioxidantandhepatoprotectiveeffectsofcapparisspinosalfractionsandquercetinontertbutylhydroperoxideinducedacuteliverdamageinmice AT parvinkheradmand antioxidantandhepatoprotectiveeffectsofcapparisspinosalfractionsandquercetinontertbutylhydroperoxideinducedacuteliverdamageinmice |