Potential hemo-biological identification markers to the left displaced abomasum in dairy cows
Abstract Background Left displaced abomasum (LDA) occurs at high frequency in the early postpartum period and can affect production performance of dairy cows. Clinical diagnosis of LDA is usually done by abdominal auscultation and percussion. The purpose of this study was to explore the potential ap...
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| Format: | Article |
| Language: | English |
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BMC
2020-12-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-020-02676-x |
| _version_ | 1818925826950823936 |
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| author | Yuxiang Song Juan J Loor Chenchen Zhao Dan Huang Xiliang Du Xiaobing Li Zhe Wang Guowen Liu Xinwei Li |
| author_facet | Yuxiang Song Juan J Loor Chenchen Zhao Dan Huang Xiliang Du Xiaobing Li Zhe Wang Guowen Liu Xinwei Li |
| author_sort | Yuxiang Song |
| collection | DOAJ |
| description | Abstract Background Left displaced abomasum (LDA) occurs at high frequency in the early postpartum period and can affect production performance of dairy cows. Clinical diagnosis of LDA is usually done by abdominal auscultation and percussion. The purpose of this study was to explore the potential applicability of blood biomarkers for early warning and diagnosis of LDA in dairy cows. Results Twenty early postpartum healthy cows and thirty early postpartum LDA cows of similar parity were used. A receiver operating characteristic curve (ROC) method was used to analyze the sensitivity of hematological biomarkers to LDA including energy balance metabolic biomarkers, liver/kidney function biomarkers, and minerals. A cut-off point was defined for each of the selected hematological biomarkers deemed sensitive markers of LDA. Compared with healthy cows, body condition score (BCS), dry matter intake (DMI) and milk production were lower in LDA cows. Among energy metabolism markers, serum non-esterified fatty acid (NEFA), β-hydroxybutyric acid (BHBA), insulin (INS), and revised quantitative insulin sensitivity check index (RQUICKI) levels were lower while serum glucagon (GC) was greater in LDA cows. Among the liver/kidney function biomarkers, activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH), the ratio of AST/ALT and levels of total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), albumin (ALB), blood urea nitrogen (BUN), creatinine, and total protein (TP) were greater in LDA cows. Among minerals analyzed, serum Cl, Ca, and K were lower in LDA cows. After ROC analysis, it was determined that serum Ca, INS, RQUICKI, ALT, GGT, and creatinine are potential indicators for early warning and diagnosis of LDA for early postpartum dairy cows. Conclusions Dairy cows with LDA were under severe negative energy balance (NEB), had signs of liver damage and potentially lower insulin sensitivity. A combination of multi-hematological biomarkers including Ca, INS, RQUICKI, ALT, GGT and creatinine has the potential to help identify cows at risk of LDA in the early postpartum period. |
| first_indexed | 2024-12-20T02:47:24Z |
| format | Article |
| id | doaj.art-7748114eeb004ca8bfb79cc671401463 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-20T02:47:24Z |
| publishDate | 2020-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-7748114eeb004ca8bfb79cc6714014632022-12-21T19:56:07ZengBMCBMC Veterinary Research1746-61482020-12-011611910.1186/s12917-020-02676-xPotential hemo-biological identification markers to the left displaced abomasum in dairy cowsYuxiang Song0Juan J Loor1Chenchen Zhao2Dan Huang3Xiliang Du4Xiaobing Li5Zhe Wang6Guowen Liu7Xinwei Li8Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin UniversityMammalian NutriPhysioGenomics, Department of Animal Sciences, Division of Nutritional Sciences, University of IllinoisKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin UniversityKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin UniversityAbstract Background Left displaced abomasum (LDA) occurs at high frequency in the early postpartum period and can affect production performance of dairy cows. Clinical diagnosis of LDA is usually done by abdominal auscultation and percussion. The purpose of this study was to explore the potential applicability of blood biomarkers for early warning and diagnosis of LDA in dairy cows. Results Twenty early postpartum healthy cows and thirty early postpartum LDA cows of similar parity were used. A receiver operating characteristic curve (ROC) method was used to analyze the sensitivity of hematological biomarkers to LDA including energy balance metabolic biomarkers, liver/kidney function biomarkers, and minerals. A cut-off point was defined for each of the selected hematological biomarkers deemed sensitive markers of LDA. Compared with healthy cows, body condition score (BCS), dry matter intake (DMI) and milk production were lower in LDA cows. Among energy metabolism markers, serum non-esterified fatty acid (NEFA), β-hydroxybutyric acid (BHBA), insulin (INS), and revised quantitative insulin sensitivity check index (RQUICKI) levels were lower while serum glucagon (GC) was greater in LDA cows. Among the liver/kidney function biomarkers, activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH), the ratio of AST/ALT and levels of total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), albumin (ALB), blood urea nitrogen (BUN), creatinine, and total protein (TP) were greater in LDA cows. Among minerals analyzed, serum Cl, Ca, and K were lower in LDA cows. After ROC analysis, it was determined that serum Ca, INS, RQUICKI, ALT, GGT, and creatinine are potential indicators for early warning and diagnosis of LDA for early postpartum dairy cows. Conclusions Dairy cows with LDA were under severe negative energy balance (NEB), had signs of liver damage and potentially lower insulin sensitivity. A combination of multi-hematological biomarkers including Ca, INS, RQUICKI, ALT, GGT and creatinine has the potential to help identify cows at risk of LDA in the early postpartum period.https://doi.org/10.1186/s12917-020-02676-xLeft displaced abomasumHematological biomarkersDairy cowDisease identificationNegative energy balanceROC curve |
| spellingShingle | Yuxiang Song Juan J Loor Chenchen Zhao Dan Huang Xiliang Du Xiaobing Li Zhe Wang Guowen Liu Xinwei Li Potential hemo-biological identification markers to the left displaced abomasum in dairy cows BMC Veterinary Research Left displaced abomasum Hematological biomarkers Dairy cow Disease identification Negative energy balance ROC curve |
| title | Potential hemo-biological identification markers to the left displaced abomasum in dairy cows |
| title_full | Potential hemo-biological identification markers to the left displaced abomasum in dairy cows |
| title_fullStr | Potential hemo-biological identification markers to the left displaced abomasum in dairy cows |
| title_full_unstemmed | Potential hemo-biological identification markers to the left displaced abomasum in dairy cows |
| title_short | Potential hemo-biological identification markers to the left displaced abomasum in dairy cows |
| title_sort | potential hemo biological identification markers to the left displaced abomasum in dairy cows |
| topic | Left displaced abomasum Hematological biomarkers Dairy cow Disease identification Negative energy balance ROC curve |
| url | https://doi.org/10.1186/s12917-020-02676-x |
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