Nicotine-induced Disturbances of Meiotic Maturation in Cultured Mouse Oocytes: Alterations of Spindle Integrity and Chromosome Alignment
We investigated whether nicotine exposure in vitro of mouse oocytes affects spindle and chromosome function during meiotic maturation (M-I and M-II). Oocytes in germinal vesicle (GV) stage were cultured in nicotine for 8 h or for 16 h, to assess effects in M-I and in metaphase II (M-II). The latter...
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| Format: | Article |
| Language: | English |
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European Publishing
2004-09-01
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| Series: | Tobacco Induced Diseases |
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| Online Access: | http://www.journalssystem.com/tid/Nicotine-induced-Disturbances-of-Meiotic-Maturation-in-Cultured-Mouse-Oocytes-Alterations-of-Spindle-Integrity-and-Chromosome-Alignment,65878,0,2.html |
| _version_ | 1818350854173884416 |
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| author | Maria Teresa Zenzes Ryszard Bielecki |
| author_facet | Maria Teresa Zenzes Ryszard Bielecki |
| author_sort | Maria Teresa Zenzes |
| collection | DOAJ |
| description | We investigated whether nicotine exposure in vitro of mouse oocytes affects spindle and chromosome function during meiotic maturation (M-I and M-II). Oocytes in germinal vesicle (GV) stage were cultured in nicotine for 8 h or for 16 h, to assess effects in M-I and in metaphase II (M-II). The latter culture setting used the three protocols: 8 h nicotine then 8 h medium (8N + 8M); 16 h nicotine (16N); 8 h medium then 8 h nicotine (8M + 8N). Non-toxic concentrations of nicotine at 1.0, 2.5, 5.0 and 10.0 mmol/L were used. Spindle-chromosome configurations were analyzed with wide-field optical sectioning microscopy. In 8 h cultures, nicotine exposure resulted in dose-related increased proportions of M-I oocytes with defective spindle-chromosome configurations. A dose-related delayed entry into anaphase I was also detected. In 16 h cultures, nicotine exposure for the first 8 h (8N + 8M), or for 16 h (16N), resulted in dose- and time-related increased proportions of oocytes arrested in M-I (10 mmol/L; 8 h: 53.2%, controls 9.6%; 16 h: 87.6%, controls 8.5%). Defects in M-I spindles and chromosomes caused M-I arrest leading to dose-related decreased proportions of oocytes that reached metaphase-II (10 mmol/L 8 h: 46.8%, controls 90.4%;16 h: 12.4%, controls 91.5%). A delayed anaphase-I affected the normal timing of M-II, leading to abnormal oocytes with dispersed chromosomes, or with double spindles and no polar body. Nicotine exposure during the second 8 h (8M + 8N) resulted in dose-related, increased proportions of M-II oocytes with defective spindles and chromosomes (10 mmol/L: 42.9%, controls 2.0%). Nicotine has no adverse effects on GV break down, but induces spindle and chromosome defects compromising oocyte meiotic maturation and development. |
| first_indexed | 2024-12-13T18:28:27Z |
| format | Article |
| id | doaj.art-774834c594c8453685ca86be69aaedca |
| institution | Directory Open Access Journal |
| issn | 1617-9625 |
| language | English |
| last_indexed | 2024-12-13T18:28:27Z |
| publishDate | 2004-09-01 |
| publisher | European Publishing |
| record_format | Article |
| series | Tobacco Induced Diseases |
| spelling | doaj.art-774834c594c8453685ca86be69aaedca2022-12-21T23:35:32ZengEuropean PublishingTobacco Induced Diseases1617-96252004-09-012September10.1186/1617-9625-2-3-15165878Nicotine-induced Disturbances of Meiotic Maturation in Cultured Mouse Oocytes: Alterations of Spindle Integrity and Chromosome AlignmentMaria Teresa Zenzes0Ryszard Bielecki1Department of Obstetrics and Gynaecology, Division of Reproductive Sciences, University of Toronto, Toronto, CanadaDepartment of Obstetrics and Gynaecology, Division of Reproductive Sciences, University of Toronto, Toronto, CanadaWe investigated whether nicotine exposure in vitro of mouse oocytes affects spindle and chromosome function during meiotic maturation (M-I and M-II). Oocytes in germinal vesicle (GV) stage were cultured in nicotine for 8 h or for 16 h, to assess effects in M-I and in metaphase II (M-II). The latter culture setting used the three protocols: 8 h nicotine then 8 h medium (8N + 8M); 16 h nicotine (16N); 8 h medium then 8 h nicotine (8M + 8N). Non-toxic concentrations of nicotine at 1.0, 2.5, 5.0 and 10.0 mmol/L were used. Spindle-chromosome configurations were analyzed with wide-field optical sectioning microscopy. In 8 h cultures, nicotine exposure resulted in dose-related increased proportions of M-I oocytes with defective spindle-chromosome configurations. A dose-related delayed entry into anaphase I was also detected. In 16 h cultures, nicotine exposure for the first 8 h (8N + 8M), or for 16 h (16N), resulted in dose- and time-related increased proportions of oocytes arrested in M-I (10 mmol/L; 8 h: 53.2%, controls 9.6%; 16 h: 87.6%, controls 8.5%). Defects in M-I spindles and chromosomes caused M-I arrest leading to dose-related decreased proportions of oocytes that reached metaphase-II (10 mmol/L 8 h: 46.8%, controls 90.4%;16 h: 12.4%, controls 91.5%). A delayed anaphase-I affected the normal timing of M-II, leading to abnormal oocytes with dispersed chromosomes, or with double spindles and no polar body. Nicotine exposure during the second 8 h (8M + 8N) resulted in dose-related, increased proportions of M-II oocytes with defective spindles and chromosomes (10 mmol/L: 42.9%, controls 2.0%). Nicotine has no adverse effects on GV break down, but induces spindle and chromosome defects compromising oocyte meiotic maturation and development.http://www.journalssystem.com/tid/Nicotine-induced-Disturbances-of-Meiotic-Maturation-in-Cultured-Mouse-Oocytes-Alterations-of-Spindle-Integrity-and-Chromosome-Alignment,65878,0,2.htmlnicotinemouse oocytenicotine exposurenicotine concentrationmeiotic spindle |
| spellingShingle | Maria Teresa Zenzes Ryszard Bielecki Nicotine-induced Disturbances of Meiotic Maturation in Cultured Mouse Oocytes: Alterations of Spindle Integrity and Chromosome Alignment Tobacco Induced Diseases nicotine mouse oocyte nicotine exposure nicotine concentration meiotic spindle |
| title | Nicotine-induced Disturbances of Meiotic Maturation in Cultured Mouse Oocytes: Alterations of Spindle Integrity and Chromosome Alignment |
| title_full | Nicotine-induced Disturbances of Meiotic Maturation in Cultured Mouse Oocytes: Alterations of Spindle Integrity and Chromosome Alignment |
| title_fullStr | Nicotine-induced Disturbances of Meiotic Maturation in Cultured Mouse Oocytes: Alterations of Spindle Integrity and Chromosome Alignment |
| title_full_unstemmed | Nicotine-induced Disturbances of Meiotic Maturation in Cultured Mouse Oocytes: Alterations of Spindle Integrity and Chromosome Alignment |
| title_short | Nicotine-induced Disturbances of Meiotic Maturation in Cultured Mouse Oocytes: Alterations of Spindle Integrity and Chromosome Alignment |
| title_sort | nicotine induced disturbances of meiotic maturation in cultured mouse oocytes alterations of spindle integrity and chromosome alignment |
| topic | nicotine mouse oocyte nicotine exposure nicotine concentration meiotic spindle |
| url | http://www.journalssystem.com/tid/Nicotine-induced-Disturbances-of-Meiotic-Maturation-in-Cultured-Mouse-Oocytes-Alterations-of-Spindle-Integrity-and-Chromosome-Alignment,65878,0,2.html |
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