Comparative Transcriptomic Analysis of <i>Riptortus pedestris</i> (Hemiptera: Alydidae) to Characterize Wing Formation across All Developmental Stages
<i>Riptortus pedestris</i> (Hemiptera: Alydidae) is a major agricultural pest in East Asia that causes considerable economic losses to the soybean crop each year. However, the molecular mechanisms governing the growth and development of <i>R. pedestris</i> have not been fully...
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MDPI AG
2021-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/3/226 |
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| author | Siying Fu Yujie Duan Siqi Wang Yipeng Ren Wenjun Bu |
| author_facet | Siying Fu Yujie Duan Siqi Wang Yipeng Ren Wenjun Bu |
| author_sort | Siying Fu |
| collection | DOAJ |
| description | <i>Riptortus pedestris</i> (Hemiptera: Alydidae) is a major agricultural pest in East Asia that causes considerable economic losses to the soybean crop each year. However, the molecular mechanisms governing the growth and development of <i>R. pedestris</i> have not been fully elucidated. In this study, the Illumina HiSeq6000 platform was employed to perform de novo transcriptome assembly and determine the gene expression profiles of this species across all developmental stages, including eggs, first-, second-, third-, fourth-, and fifth-instar nymphs, and adults. In this study, a total of 60,058 unigenes were assembled from numerous raw reads, exhibiting an N50 length of 2126 bp and an average length of 1199 bp, and the unigenes were annotated and classified with various databases, such as the Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), and Gene Ontology (GO). Furthermore, various numbers of differentially expressed genes (DEGs) were calculated through pairwise comparisons of all life stages, and some of these DEGs were associated with immunity, metabolism, and development by GO and KEGG enrichment. In addition, 35,158 simple sequence repeats (SSRs) and 715,604 potential single nucleotide polymorphisms (SNPs) were identified from the seven transcriptome libraries of <i>R. pedestris</i>. Finally, we identified and summarized ten wing formation-related signaling pathways, and the molecular properties and expression levels of five wing development-related genes were analyzed using quantitative real-time PCR for all developmental stages of <i>R. pedestris</i>. Taken together, the results of this study may establish a foundation for future research investigating developmental processes and wing formation in hemimetabolous insects and may provide valuable data for pest control efforts attempting to reduce the economic damage caused by this pest. |
| first_indexed | 2024-03-09T05:17:14Z |
| format | Article |
| id | doaj.art-774e1cc69bba41b5bb82069f823ec34d |
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| language | English |
| last_indexed | 2024-03-09T05:17:14Z |
| publishDate | 2021-03-01 |
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| series | Insects |
| spelling | doaj.art-774e1cc69bba41b5bb82069f823ec34d2023-12-03T12:44:16ZengMDPI AGInsects2075-44502021-03-0112322610.3390/insects12030226Comparative Transcriptomic Analysis of <i>Riptortus pedestris</i> (Hemiptera: Alydidae) to Characterize Wing Formation across All Developmental StagesSiying Fu0Yujie Duan1Siqi Wang2Yipeng Ren3Wenjun Bu4Institute of Entomology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaInstitute of Entomology, College of Life Sciences, Nankai University, Tianjin 300071, China<i>Riptortus pedestris</i> (Hemiptera: Alydidae) is a major agricultural pest in East Asia that causes considerable economic losses to the soybean crop each year. However, the molecular mechanisms governing the growth and development of <i>R. pedestris</i> have not been fully elucidated. In this study, the Illumina HiSeq6000 platform was employed to perform de novo transcriptome assembly and determine the gene expression profiles of this species across all developmental stages, including eggs, first-, second-, third-, fourth-, and fifth-instar nymphs, and adults. In this study, a total of 60,058 unigenes were assembled from numerous raw reads, exhibiting an N50 length of 2126 bp and an average length of 1199 bp, and the unigenes were annotated and classified with various databases, such as the Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), and Gene Ontology (GO). Furthermore, various numbers of differentially expressed genes (DEGs) were calculated through pairwise comparisons of all life stages, and some of these DEGs were associated with immunity, metabolism, and development by GO and KEGG enrichment. In addition, 35,158 simple sequence repeats (SSRs) and 715,604 potential single nucleotide polymorphisms (SNPs) were identified from the seven transcriptome libraries of <i>R. pedestris</i>. Finally, we identified and summarized ten wing formation-related signaling pathways, and the molecular properties and expression levels of five wing development-related genes were analyzed using quantitative real-time PCR for all developmental stages of <i>R. pedestris</i>. Taken together, the results of this study may establish a foundation for future research investigating developmental processes and wing formation in hemimetabolous insects and may provide valuable data for pest control efforts attempting to reduce the economic damage caused by this pest.https://www.mdpi.com/2075-4450/12/3/226<i>Riptortus pedestris</i>transcriptome librarywing formationexpression levelspest control |
| spellingShingle | Siying Fu Yujie Duan Siqi Wang Yipeng Ren Wenjun Bu Comparative Transcriptomic Analysis of <i>Riptortus pedestris</i> (Hemiptera: Alydidae) to Characterize Wing Formation across All Developmental Stages Insects <i>Riptortus pedestris</i> transcriptome library wing formation expression levels pest control |
| title | Comparative Transcriptomic Analysis of <i>Riptortus pedestris</i> (Hemiptera: Alydidae) to Characterize Wing Formation across All Developmental Stages |
| title_full | Comparative Transcriptomic Analysis of <i>Riptortus pedestris</i> (Hemiptera: Alydidae) to Characterize Wing Formation across All Developmental Stages |
| title_fullStr | Comparative Transcriptomic Analysis of <i>Riptortus pedestris</i> (Hemiptera: Alydidae) to Characterize Wing Formation across All Developmental Stages |
| title_full_unstemmed | Comparative Transcriptomic Analysis of <i>Riptortus pedestris</i> (Hemiptera: Alydidae) to Characterize Wing Formation across All Developmental Stages |
| title_short | Comparative Transcriptomic Analysis of <i>Riptortus pedestris</i> (Hemiptera: Alydidae) to Characterize Wing Formation across All Developmental Stages |
| title_sort | comparative transcriptomic analysis of i riptortus pedestris i hemiptera alydidae to characterize wing formation across all developmental stages |
| topic | <i>Riptortus pedestris</i> transcriptome library wing formation expression levels pest control |
| url | https://www.mdpi.com/2075-4450/12/3/226 |
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